Tropical Animal Health and Production

, Volume 51, Issue 8, pp 2641–2644 | Cite as

Embryo production in middle-aged and mature Bos taurus × Bos indicus cows induced to multiple ovulation in a tropical environment

  • Fernando Naranjo-Chacón
  • Felipe Montiel-PalaciosEmail author
  • Rodolfo Canseco-Sedano
  • Concepción Ahuja-Aguirre
Short Communications


The objective of the study was to evaluate embryo production in middle-aged and mature Bos taurus × Bos indicus cows induced to multiple ovulation (MO) in a tropical environment. Twenty-eight cows were assigned into two groups: (1) middle-aged cows = 4–6 years old (n = 13), and (2) mature cows = 8–12 years old (n = 15). All donors received the same MO protocol with follicle-stimulating hormone in decreasing dose during 4 days and two artificial insemination services. Total numbers of corpora lutea at embryo collection, structures collected, and viable embryos obtained, as well as recovery rate, were higher in middle-aged cows compared with mature cows (P < 0.05). A total number of degenerate embryos and unfertilized oocytes, as well as viability rate, were similar in both groups (P > 0.05). In conclusion, the mature cows responded to the MO treatment, but the average of viable embryos recovered per donor was lower than in middle-aged cows. Therefore, the inclusion of cows ≥ 8 years old as donors in MO programs in tropical environments should be avoided.


Dual-purpose cattle Embryo donor FSH Superovulation 



The study was funded by Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana (FMVZ-UV) through project 18547 “Servicios en Reproducción Animal,” and by Mr. Ramón Fuster and Mr. Timoteo Aguirre.

Compliance with ethical standards

The Bioethics and Animal Welfare Commission of the FMVZ-UV approved the use of animals and the procedures included in the study.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Facultad de Medicina Veterinaria y ZootecniaUniversidad VeracruzanaVeracruzMexico

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