Phenotypic and genotypic characteristics of Brucella isolates from the Republic of Kazakhstan
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The purposes of this study were to determine phenotypic and genotypic characteristics of Brucella isolates from the Republic of Kazakhstan and to determine their biotype. The focus was laid on culture-morphological, biochemical, and biological properties of 59 Brucella isolates from primary cultures. Material was isolated from blood and tissue of serum-positive killed, dead diseased, or aborted domestic cattle from different regions of Kazakhstan where brucellosis is a common problem. Multiple-locus variable number tandem repeat analysis (MLVA) of all strains, isolated in different regions, has shown that Brucella isolates from the epizootic form two clusters. Based on the comparison with strains available in the MLVA database, B. abortus 0015/B is alike the B. abortus strains isolated from Italy and Portugal. B. melitensis 0016/B isolated from the Almaty region fits the third cluster and is alike the B. melitensis strains isolated from humans in Turkey, China, and Portugal. More than 90% of the overall B. abortus samples were isolated from the northern regions of the East and West Kazakhstan, while B. melitensis strains were registered in the southeast Kazakhstan. The most frequently recorded B. abortus biovar is biovar 3. The most frequently recorded B. melitensis biovars are biovars 1 and 3.
Significance and impact of study
These results contribute to a better understanding of the geographic pattern of Brucella infection in Kazakh cattle also important for developing the specific control measures. The results of current research can be used for creating a gene bank of Brucella strains circulating in Kazakhstan for producing diagnostic and therapeutic agents. The research material will be used to solve the problems of genetic characterization of Brucella species and to establish the phylogenetic relationships of strains.
KeywordsBrucellosis Infection monitoring Typing of Brucella isolates Phenotypic Genotypic R- and S-dissociation B. melitensis B. abortus Multiple-locus variable number tandem repeat analysis (MLVA)
The research was carried out within the framework of the Republican Scientific and Technical Program No. O.0600 Brucellosis in Cattle: Epizootic Monitoring, Diagnostic and Prevention Tools Design (2012-2014), supported by the Ministry of Education and Science of the Republic of Kazakhstan. We would like to express our gratitude to the Laboratory of Molecular Biology and Genetic Engineering, namely Professor Zaitsev Valentin, the senior researcher Strochkov Vitaliy, and the Head of the Laboratory Sultankulova Kulyaisan, for assisting in electron microscopy, as well as with the AMOS-PCR and molecular typing of Brucella isolates, based on MLVA analysis.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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