Pathological and microbiological characterization of mastitis in dairy cows
- 178 Downloads
Mastitis may be caused by a wide range of microorganisms able to induce distinct lesions in mammary tissues. This study aims to characterize the gross and microscopic features of mastitis in dairy cows and to correlate them with the pathogens involved. The udders of slaughtered dairy cows were inspected and milk samples from each mammary quarter or fragments of the parenchyma were sent for microbiological analysis, and tissue collected for histopathological evaluation. A total of 148 cows and 592 mammary quarters were collected. From these, 432 quarters (73%) had mastitis and in 160 (27%), no changes were observed. Mastitis was classified into seven patterns based on the histopathological findings, of which mixed, lymphoplasmacytic, and suppurative mastitides were the most prevalent with 35.9% (155/432), 27.1% (117/432), and 14.3% (62/432) of the cases, respectively. These patterns were associated with the same set of pathogens: Streptococcus spp., coagulase-negative Staphylococcus (CNS), Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis, and Corynebacterium bovis. The pyogranulomatous pattern represented 7.2% (31/432) of the cases with distinct distribution based on the agent involved, mostly S. aureus and Nocardia sp. Abscedative mastitis accounted for 6.0% (26/432) of the cases; it was characterized by multiple abscesses in the parenchyma and was mainly caused by Trueperella pyogenes. Necrosuppurative mastitis represented 5.8% (25/432) of the cases which were characterized by severe parenchyma necrosis and were caused by bacteria such as CNS and Escherichia coli. The granulomatous pattern represented 3.7% (16/432) of the cases and was occasionally associated with Mycobacterium sp.
KeywordsMammary gland Dairy cattle Mammary pathology Bacteria
This study was financially supported by the Conselho Nacional de Desenvolvimento Científico e Tecnólogico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Compliance with ethical standards
Statement of animal rights
The project that gave rise to the present data was approved to the Research Committee (COMPESQ) of the Universidade Federal do Rio Grande do Sul (UFRGS) (Project number 33527). The manuscript does not contain clinical studies or patient data.
Conflict of interest
The authors declare that they have no conflicts of interest.
- Acosta, A.C., Silva, L.B.G., Medeiros, E.S., Pinheiro-Júnior, J.W., Mota, R.A., 2016. Mastitis in ruminants in Brazil. Pesquisa Veterinária Brasileira, 36, 565–573.Google Scholar
- Anaya-López, J.L., Contreras-Guzmán, O.E., Cárabez-Trejo, A., Baizabal-Aguirre, V.M., López-Meza, J.E., Valdez-Alarcón, J.J., Ochoa-Zarzosa, A., 2006. Invasive potential of bacterial isolates associated with subclinical bovine mastitis. Research in Veterinary Science, 81, 358–361.CrossRefGoogle Scholar
- Cunha, A.F., Bragança, L.J., Quintão, L.C., Silva, S.Q., Souza, F.N., Cerqueira, M.M.O.P., 2015. Prevalence, etiology and risk factors of subclinical mastitis in dairy cattle of Viçosa-MG. Acta Veterinaria Brasilica, 9, 160–166.Google Scholar
- Donovan, G.A., Gross, T.L., 1984. Cutaneous botryomycosis (bacterial granulomas) in dairy cows caused by Pseudomonas aeruginosa. Journal of the American Veterinary Medical Association, 184, 197–199.Google Scholar
- Hazlett, M.J., Little, P.B., Maxie, M.G., Barnum, D.A., 1984. Fatal mastitis of dairy cows: a retrospective study. The Canadian Journal of Comparative Medicine, 48, 125–129.Google Scholar
- Hussian, R., Javed, M.T., Khan, A., Mahmood, F., Kausar, R., 2012. Mastitis and associated histopathological consequences in the context of udder morphology. International Journal of Agriculture and Biology, 14, 947–952.Google Scholar
- Ishiyama, D., Mizomoto, T., Ueda, C., Takagi, N., Shimizu, N., Matsuura, Y., Matsuura, Y., Makuuchi, Y., Watanabe, A., Shinozuka, Y., Kawai, K., 2017. Factors affecting the incidence and outcome of Trueperella pyogenes mastitis in cows. The Journal of Veterinary Medical Science, 79, 626–631.CrossRefGoogle Scholar
- Markey B, Leonard F, Archambault M, Cullinane A, Maguire, D., 2013a. Bacterial pathogens: microscopy, culture and identification. In: Ibid (eds), Clinical Veterinary Microbiology, 2nd ed, (Elsevier, St. Louis, MO), p. 9-48a.Google Scholar
- Markey B, Leonard F, Archambault M, Cullinane A, Maguire, D., 2013b. Mastitis. In: Ibid (eds), Clinical Veterinary Microbiology, 2nd ed, (Elsevier, St. Louis, MO), p. 433-453b.Google Scholar
- National Mastitis Council, 1999. Laboratory handbook on bovine mastitis, (NMC Inc., Madison, WI).Google Scholar
- Schiefer, B., Macdonald, K.R., Klavano, G.G., Dreumel, A.A., 1976. Pathology of Bacillus cereus mastitis in dairy cows. The Canadian Veterinary Journal, 17, 239–243.Google Scholar
- Shibahara, T., Nakamura, K., 1999. Pathology of acute necrotizing mastitis caused by Staphylococcus aureus in a dairy cow. The Japan Agricultural Research Quarterly, 33, 139–142.Google Scholar
- Shibahara, T., Akiba, T., Maeda, T., Ogata, T., Honda, R., Ishikawa, Y., Kadota, K., 2002. Immunohistochemical and ultrastructural identification of Fusobacterium necrophorum subsp. necrophorum in bovine fatal necrotizing glossitis. The Journal of Veterinary Medical Science, 64, 523–526.CrossRefGoogle Scholar