In vitro evaluations of pellets containing Delonix regia seed meal for ruminants
- 15 Downloads
The objective of the present research was to determine the influence of various doses of the pellets containing DR seed meal (PEDEM) on in vitro CH4 gas production, in vitro digestibility, protozoal count, and ruminal fermentation characteristics. The study was designed as a completely randomized design with eight levels of PEDEM supplementation at 0, 2, 4, 6, 8, 10, 12, and 14 mg DM. Gas production rate constants for the insoluble fraction (c) and cumulative gas production at 96 h were quadratically increased when PEDEM was supplemented (P < 0.05). The concentration of NH3-N was linearly increased when the PEDEM concentration (P < 0.05) was increased, whereas the population of protozoa was linearly decreased when the level of PEDEM supplementation (P < 0.05) was increased. The supplementation of PEDEM in substrate quadratically affected the mean values of in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), and in vitro NDF digestibility (P < 0.05). TVFA, acetic acid (C2), and butyric acid (C4) were not altered by different doses of PEDEM supplementation (P > 0.05). In contrast, the concentration of propionic acid (C3) was quadratically affected with the supplementation of PEDEM (P = 0.05). The inclusion of PEDEM did not change the CH4 concentration at 6 h of incubation (P > 0.05), whereas the CH4 concentration at 24 h of incubation and the mean values were linearly reduced with additional doses of PEDEM (P < 0.05). Compared with the control group, the mean CH4 concentration was reduced at 51.1% with 12 mg PEDEM, whereas 59.6% was reduced with 14 mg PEDEM supplementations. The supplementation of PEDEM at 12 mg has the potential to manipulate rumen fermentation, to manipulate in vitro digestibility and to reduce protozoa and CH4 production.
KeywordsPlant secondary compound Rumen fermentation Greenhouse gas Protozoa Feed processing
The authors would like to express their most sincere thanks to the Increase Production Efficiency and Meat Quality of Native Beef and Buffalo Research Group, Khon Kaen University (KKU), for the use of the research facilities.
This study is financially supported by the Thailand Research Fund (TRF) and Office of the Commission on Higher Education through the Research Grant for New Scholar (grant no. MRG6080003) and Increase Production Efficiency and Meat Quality of Native Beef and Buffalo Research Group, Khon Kaen University (KKU). This work was also supported by the TRF though the Research and Researcher for Industry (RRi) program (contract grants PHD60I0080) and TFR-IRG5980010.
- Association of Official Analytical Chemists (AOAC). 1998. Official Analytical Chemists 16th ed. Arlington, Virginia.Google Scholar
- Calabrò S, Cutrignelli MI, Lo Presti V, Tudisco R, Chiofalo V, Grossi M, Infascelli F, Chiofalo B. 2015. Characterization and effect of year at harvest on the nutritional properties of three varieties of white lupine (Lupinus albus L.). Journal of Science Food and Agriculture, 95, 3127–3136CrossRefGoogle Scholar
- Calabrò S, Cutrignelli MI, Piccolo G, Bovera F, Zicarelli F, Gazaneo MP, Infascelli F. 2005b. In vitro fermentation kinetics of fresh and dried silage Animal Feed Science and Technology, 123–124, 129–137.Google Scholar
- Cieślak A, Zmora P, Matkowski A, Nawrot-Hadzik I, Pers-Kamczyc E, El-Sherbiny M, Bryszak M, Szumacher-Strabe M. 2016. Tannins from Sanguisorba officinalis affect in vitro rumen methane production and fermentation. Journal of Animal and Plant Sciences, 26, 54–62.Google Scholar
- Foiklang S, Wanapat M, Norrapoke T. 2016. Effect of grape pomace powder, mangosteen peel powder and monensin on nutrient digestibility, rumen fermentation, nitrogen balance and microbial protein synthesis in dairy steers. Asian-Australasian Journal of Animal Sciences, 29, 1416–1423.CrossRefGoogle Scholar
- Gunun P, Gunun N, Khejornsart P, Ouppamong T, Cherdthong A, Wanapat M, Sirilaophaisan S, Yuangklang C, Polyorach S, Kenchaiwong W, Kang S. 2019. Effects of Antidesma thwaitesianum Muell. Arg. pomace as a source of plant secondary compounds on digestibility, rumen environment, hematology and milk production in dairy cows. Animal Science Journal, 90, 372–381.CrossRefGoogle Scholar
- Menke KH, Steingass H. 1988. Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid. Animal Research Development, 28, 7–55.Google Scholar
- SAS. 1998. User’s guide: statistics, Version6, 12th Edition. SAS Inst. Inc., Cary, NC.Google Scholar
- Seankamsorn A, Cherdthong A, Wanapat M, Supapong C, Khonkhaeng B, Uriyapongson S, Gunun N, Gunun P, Chanjula P. 2017. Effect of dried rumen digesta pellet levels on feed use, rumen ecology, and blood metabolite in swamp buffalo. Tropical Animal Health and Production, 48, 79–86.CrossRefGoogle Scholar
- Śliwiński BJ, Kreuzer M, Wettstein H-R, Machmüller A. 2002. Rumen fermentation and nitrogen balance of lambs fed diets containing plant extracts rich in tannins and saponins, and associated emissions of nitrogen and methane. Archives of Animal Nutrition, 56, 379–392.Google Scholar
- Südekum K-H, Schröder A, Fiebelkorn S, Schwer R, Thalmann A. 2008. Quality characteristics of pelleted compound feeds under varying storage conditions as influenced by purity and concentration of glycerol from biodiesel production. Journal of Animal and Feed Sciences, 17, 120–136.CrossRefGoogle Scholar
- Supapong C, Cherdthong A, Seankamsorn A, Khonkhaeng B, Wanapat M, Uriyapongson S, Gunun N, Gunun P, Chanjula P, Polyorach S. 2017. In vitro fermentation, digestibility and methane production as influenced by Delonix regia seed meal containing tannins and saponins. Journal of Animal and Feed Sciences, 26, 123–130.CrossRefGoogle Scholar