Semen quality of preserved buffalo semen extended in tris extender with processed yolk enriched with strawberry juice (Fragaria spp.)

  • Walid S. El-NattatEmail author
  • Reda I. El-Sheshtawy
Regular Articles


Strawberry fruit is rich in natural antioxidants supplemented as a preservative in semen extenders. There is little literature regarding its application in cattle bulls. So, the aim of the present study was to explore the impact of strawberry juice beside LDL derived from egg yolk on buffalo spermatozoa during cooling and cryopreservation. Collected semen was pooled to be divided in aliquots. Semen was diluted in tris-Citrate-Fructose-yolk low-density lipoprotein (TLCF) (control, 0% strawberry juice (SB)) and in six different concentrations (1–6%) of tris-LDL-strawberry diluent (TLSB) to ensure 60 million motile spermatozoa/mL. The cooled diluted semen in 0.25-mL polyvinyl French straws were processed and cryopreserved in liquid nitrogen (LN2). Extended semen was evaluated (motility %, alive %, abnormality %, and normal sperm membrane (HOST) %) in the preserved semen. Data output revealed that sperm motility of post-chilled spermatozoa improved on adding strawberry concentrations 3 and 4% in TLCF extender. In frozen semen, SB concentration 1 and 2% gave the best significance (P < 0.0014) sperm motility relative to the control. With respect to the alive sperm percent, the majority of the concentrations used showed significant higher alive sperm relative to the control. The sperm abnormalities was significant (P < 0.0001) lower in most of the supplemented concentrations relative to the control. HOST percentage was kept in every concentration. It could be concluded that enrichment of 1–4% strawberry juice in tris-LDL extender improved semen quality post-cooling and post-freezing.


Buffalo Preservation Semen Strawberry 


Funding information

This work was supported by the National Research Centre [grant numbers: 10120801].


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Animal Reproduction and Artificial Insemination DepartmentNational Research CentreDokkiEgypt

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