Advertisement

Multiple injections of vitamin E and selenium improved the reproductive performance of estrus-synchronized Awassi ewes

  • M. S. AwawdehEmail author
  • A. H. Eljarah
  • M. M. Ababneh
Regular Articles
  • 15 Downloads

Abstract

The aim of the current study was to investigate the effects of injecting estrus-synchronized ewes with vitamin E and selenium (Se) on their reproductive performance. Awassi ewes (n = 74) were randomly assigned into one of two groups. Group one (control, n = 36) did not receive vitamin E/Se injections, and group two (vitamin E/Se, n = 38) received 13.6-mg/kg BW of vitamin E plus 0.045-mg/kg BW of Se. Concurrent with estrus-synchronization program, vitamin E/Se injections were given at the time of insertion, withdrawal, and 19 days after withdrawal of intravaginal sponges. At all injection times, serum samples were collected (20 ewes per group) to measure Se contents. Pregnancy rates were evaluated by progesterone assay and by ultrasonography, respectively, at days 19 and 40 after sponge removal. Lambing rate, singles and twins%, sex ratio (M:F), and birth weight were recorded at lambing. Vitamin E/Se injections did not affect (P > 0.25) BW at lambing or BW change of ewes from breeding to lambing. Vitamin E/Se injections tended (P = 0.08) to decrease total pregnancy losses from 44.8 to 24.3%, subsequently, injections positively improved (P < 0.05) pregnancy rates determined by progesterone assay (from 80.6 to 97.4%) and ultrasonography (from 63.9 to 86.8%). Although overall fertility was not affected, vitamin E/Se injections markedly increased the percentage of ewes that lambed after only one service from 64.0 to 93.3%. Singles and twins%, lamb sex ratio, and birth weight of lambs were not affected (P > 0.20) by vitamin E/Se injections. Under conditions of our study, multiple injections of vitamin E/Se improved the reproductive performance of estrus-synchronized ewes.

Keywords

Pregnancy rate Selenium Sheep reproduction Vitamin E 

Abbreviations

ARTU

Agricultural Research and Training Unit

BW

Body weight

JUST

Jordan University of Science and Technology

P4

Progesterone

Se

Selenium

Notes

Funding information

Authors would like to thank the Deanship of Scientific Research at JUST for funding this project (#201/2010). Thanks are due to the manager and staff of ARTU at JUST.

Compliance with ethical standards

The Institutional Animal Care and Use Committee at JUST approved all procedures used in this study.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Awawdeh, M. S. and A. Q. Talafha. 2015. Blood and milk status of vitamin E, vitamin A, and selenium in nursing Awassi ewes injected with vitamin E and selenium. Acta Agriculturae Scandinavica, Section A-Animal Science, 65, 176–182.CrossRefGoogle Scholar
  2. Awawdeh, M. A., Talafha, A. and B. S. Obeidat. 2015. Postpartum injection with vitamin E and selenium failed to improve the performance of Awassi ewes and their lambs. Canadian Journal of Animal Science, 95, 111–115.CrossRefGoogle Scholar
  3. Chemineau, P., Pellicer-Rubio, M., Lassoued, N., Khaldi, G. and Monniaux, D., 2006. Influence of nutrition and socio-sexual context on reproduction and survival of the young in goats and sheep. Reproduction Nutrition Development, 46, 417–429.CrossRefGoogle Scholar
  4. El-Shahat, K.H. and Abdel Monem, U.M., 2011. Effects of dietary supplementation with vitamin E and/or selenium on metabolic and reproductive performance of Egyptian baladi ewes under subtropical conditions. World Applied Sciences Journal, 12, 1492–1499.Google Scholar
  5. Gabryszuk, M. and Klewiec, J., 2002. Effect of injecting 2- and 3-year-old ewes with selenium and selenium–vitamin E on reproduction and rearing of lambs. Small Ruminant Research, 43, 127–132.CrossRefGoogle Scholar
  6. Haddadin, M.S.Y., Khattari, S., Caretto, D. and Robinson, R.K., 2001, Potential intake of selenium by the inhabitants of different regions in Jordan. Nutrition and Food Science, 31, 230–233.CrossRefGoogle Scholar
  7. Hatfield, P.G., Daniels, J.T., Kott, R.W., Burgess, B.E. and Evans, T.J.. 2000. Role of supplemental vitamin E in lamb survival and production: a review. Journal of Animal Science, 77, 1–9.CrossRefGoogle Scholar
  8. Hefnawy, A.E.G., and Tórtora-Pérez, J.L., 2010. The importance of selenium and the effects of its deficiency in animal health. Small Ruminant Research, 89, 185–192.CrossRefGoogle Scholar
  9. Hefnawy, A.E.G., López-Arellano, R., Revilla-Vázquez, A., Ramírez-Bribiesca, E. and Tórtora-Pérez, J., 2007. The relationship between fetal and maternal selenium concentrations in sheep and goats. Small Ruminant Research, 73, 174–180.CrossRefGoogle Scholar
  10. Hemingway, R.G., 2003. The influences of dietary intakes and supplementation with selenium and vitamin E on reproduction diseases and reproductive efficiency in cattle and sheep. Veterinary Research Communications, 27, 159–174.CrossRefGoogle Scholar
  11. Koller, L.D., Whitbeck, G.A., and South, P.J., 1984. Transplacental transfer and colostral concentrations of selenium in beef cattle. American Journal of Veterinary Research, 45, 2507–2510.Google Scholar
  12. Köse, M., Kirbaș, M., Dursun, S. and Bayril, T., 2013. The effect of injections of β-carotene or vitamin E+Selenium on fertility in ewes in anestrus season. Kafkas University Veterinary Faculty Magazine, 24, 83–86.Google Scholar
  13. Koyuncu, M., and Yerlikaya, H., 2007. Effect of selenium-vitamin E injections of ewes on reproduction and growth of their lambs. South African Journal of Animal Science, 37, 233–236.CrossRefGoogle Scholar
  14. Kuisi, M.A., and Abdel-Fattah, A., 2010. Groundwater vulnerability to selenium in semi-arid environments: Amman Zarqa Basin, Jordan. Environmental Geochemistry and Health, 32, 107–128.CrossRefGoogle Scholar
  15. Kuru, M., Ögün, M., Oral, H., Kükürt, A., Makav, M. and Kulaksız, R., 2016. The use of controlled internal drug release for synchronization augmented oxidative and nitrosative stress and leptin levels in Georgian goats. Journal of Cellular Neuroscience and Oxidative Stress. 8, 541–542.Google Scholar
  16. Kuru, M., Sogukpinar, O., Makav, M. and Centin, N., 2017. Effect of barium selenate injections on fertility of Pirlak ewes subjected to estrus synchronization during non-breeding season. Medycyna Weterynary, 73, 479–482.CrossRefGoogle Scholar
  17. Liu, S. et al., 2014. Vitamin E status and reproduction in sheep: potential implications for Australian sheep production. Animal Production Science, 54, 694–714.CrossRefGoogle Scholar
  18. McDowell, L.R. et al., 1996. Vitamin E supplementation for the ruminant. Animal Feed Science and Technology, 60, 273–296.CrossRefGoogle Scholar
  19. Meschy, F., 2000. Recent progress in the assessment of mineral requirements of goats. Livestock Production Science, 64, 9–14.CrossRefGoogle Scholar
  20. Munoz, C. et al., 2008. Nutritional status of adult ewes during early and mid-pregnancy. 2. Effects of supplementation with selenised yeast on ewe reproduction and offspring performance to weaning. Animal, 2, 64–72.Google Scholar
  21. National Research Council, NRC (2007) Nutrient Requirements of Small Ruminants: Sheep, Goats, Cervids, and New World Camelids (The National Academies Press: Washington, DC).Google Scholar
  22. Pearce, D.T., Martin, G.B. and Oldham, C.M., 1985. Corpora lutea with a short life-span induced by rams in seasonally anovulatory ewes are prevented by progesterone delaying the preovulatory surge of LH. Journal of Reproduction and Fertility, 75, 79–84.CrossRefGoogle Scholar
  23. Ramírez-Bribiesca, J.E. et al., 2005. Effect of selenium-vitamin E injection in selenium-deficient dairy goats and kids on the Mexican plateau. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 57, 77–84.CrossRefGoogle Scholar
  24. Rooke, J.A., Dwyer, C.M., and Ashworth, C.J., 2008. The potential for improving physiological, behavioural and immunological responses in the neonatal lamb by trace element and vitamin supplementation of the ewe. Animal, 2, 514–524.Google Scholar
  25. Segerson, E.C., and Ganapathy, S.N., 1980. Fertilization of ova in selenium/vitamin E-treated ewes maintained on two planes of nutrition. Journal of Anim Science. 51, 386–394.CrossRefGoogle Scholar
  26. Segerson, E.C., Gunsett, F.C., and Getz, W.R., 1986. Selenium-vitamin E supplementation and production efficiency in ewes marginally deficient in selenium. Livestock Production Science, 14, 149–159.Google Scholar
  27. Sönmez, M., Bozkurt, T., Türk, G., Gür, S., Kizil, M. and Yüce, A., 2009. The effect of vitamin E treatment during preovulatory period on reproductive performance of goats following estrous synchronization using intravaginal sponges. Animal Reproduction Science, 114, 183–192.CrossRefGoogle Scholar
  28. Talafha, A.Q., and Ababneh, M.M., 2011. Awassi sheep reproduction and milk production: review. Tropical Animal Health and Production,43, 1319–1326.CrossRefGoogle Scholar
  29. Underwood, E.J., and Suttle, N.F., 1999. The mineral nutrition of livestock 1999. (CABI publishing Wallingford).CrossRefGoogle Scholar
  30. Zanão, R.A., Barbosa Jr., F., Souza, S.S., Krug, F.J.and Abdalla, A.L., 2002. Direct determination of selenium in whole blood by electrothermal atomic absorption spectrometry using W–Rh-coated platform and co-injection of Rh as thermal stabilizer. Spectrochimica Acta Part B: Atomic Spectroscopy, 57, 291–301.CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • M. S. Awawdeh
    • 1
    Email author
  • A. H. Eljarah
    • 2
  • M. M. Ababneh
    • 2
  1. 1.Department of Veterinary Pathology and Public HealthJordan University of Science and TechnologyIrbidJordan
  2. 2.Department of Clinical Veterinary Medical Sciences, Faculty of Veterinary MedicineJordan University of Science and TechnologyIrbidJordan

Personalised recommendations