Multiple injections of vitamin E and selenium improved the reproductive performance of estrus-synchronized Awassi ewes
- 15 Downloads
The aim of the current study was to investigate the effects of injecting estrus-synchronized ewes with vitamin E and selenium (Se) on their reproductive performance. Awassi ewes (n = 74) were randomly assigned into one of two groups. Group one (control, n = 36) did not receive vitamin E/Se injections, and group two (vitamin E/Se, n = 38) received 13.6-mg/kg BW of vitamin E plus 0.045-mg/kg BW of Se. Concurrent with estrus-synchronization program, vitamin E/Se injections were given at the time of insertion, withdrawal, and 19 days after withdrawal of intravaginal sponges. At all injection times, serum samples were collected (20 ewes per group) to measure Se contents. Pregnancy rates were evaluated by progesterone assay and by ultrasonography, respectively, at days 19 and 40 after sponge removal. Lambing rate, singles and twins%, sex ratio (M:F), and birth weight were recorded at lambing. Vitamin E/Se injections did not affect (P > 0.25) BW at lambing or BW change of ewes from breeding to lambing. Vitamin E/Se injections tended (P = 0.08) to decrease total pregnancy losses from 44.8 to 24.3%, subsequently, injections positively improved (P < 0.05) pregnancy rates determined by progesterone assay (from 80.6 to 97.4%) and ultrasonography (from 63.9 to 86.8%). Although overall fertility was not affected, vitamin E/Se injections markedly increased the percentage of ewes that lambed after only one service from 64.0 to 93.3%. Singles and twins%, lamb sex ratio, and birth weight of lambs were not affected (P > 0.20) by vitamin E/Se injections. Under conditions of our study, multiple injections of vitamin E/Se improved the reproductive performance of estrus-synchronized ewes.
KeywordsPregnancy rate Selenium Sheep reproduction Vitamin E
Agricultural Research and Training Unit
Jordan University of Science and Technology
Authors would like to thank the Deanship of Scientific Research at JUST for funding this project (#201/2010). Thanks are due to the manager and staff of ARTU at JUST.
Compliance with ethical standards
The Institutional Animal Care and Use Committee at JUST approved all procedures used in this study.
Conflict of interest
The authors declare that they have no conflict of interest.
- El-Shahat, K.H. and Abdel Monem, U.M., 2011. Effects of dietary supplementation with vitamin E and/or selenium on metabolic and reproductive performance of Egyptian baladi ewes under subtropical conditions. World Applied Sciences Journal, 12, 1492–1499.Google Scholar
- Koller, L.D., Whitbeck, G.A., and South, P.J., 1984. Transplacental transfer and colostral concentrations of selenium in beef cattle. American Journal of Veterinary Research, 45, 2507–2510.Google Scholar
- Köse, M., Kirbaș, M., Dursun, S. and Bayril, T., 2013. The effect of injections of β-carotene or vitamin E+Selenium on fertility in ewes in anestrus season. Kafkas University Veterinary Faculty Magazine, 24, 83–86.Google Scholar
- Kuru, M., Ögün, M., Oral, H., Kükürt, A., Makav, M. and Kulaksız, R., 2016. The use of controlled internal drug release for synchronization augmented oxidative and nitrosative stress and leptin levels in Georgian goats. Journal of Cellular Neuroscience and Oxidative Stress. 8, 541–542.Google Scholar
- Munoz, C. et al., 2008. Nutritional status of adult ewes during early and mid-pregnancy. 2. Effects of supplementation with selenised yeast on ewe reproduction and offspring performance to weaning. Animal, 2, 64–72.Google Scholar
- National Research Council, NRC (2007) Nutrient Requirements of Small Ruminants: Sheep, Goats, Cervids, and New World Camelids (The National Academies Press: Washington, DC).Google Scholar
- Rooke, J.A., Dwyer, C.M., and Ashworth, C.J., 2008. The potential for improving physiological, behavioural and immunological responses in the neonatal lamb by trace element and vitamin supplementation of the ewe. Animal, 2, 514–524.Google Scholar
- Segerson, E.C., Gunsett, F.C., and Getz, W.R., 1986. Selenium-vitamin E supplementation and production efficiency in ewes marginally deficient in selenium. Livestock Production Science, 14, 149–159.Google Scholar
- Zanão, R.A., Barbosa Jr., F., Souza, S.S., Krug, F.J.and Abdalla, A.L., 2002. Direct determination of selenium in whole blood by electrothermal atomic absorption spectrometry using W–Rh-coated platform and co-injection of Rh as thermal stabilizer. Spectrochimica Acta Part B: Atomic Spectroscopy, 57, 291–301.CrossRefGoogle Scholar