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Effects of dietary supplementation of bentonite and Saccharomyces cerevisiae cell wall on acute-phase protein and liver function in high-producing dairy cows during transition period

  • Seyed Amin Razavi
  • Mehrdad Pourjafar
  • Ali HajimohammadiEmail author
  • Reza Valizadeh
  • Abbas Ali Naserian
  • Richard Laven
  • Kristina Ruth Mueller
Regular Articles
  • 18 Downloads

Abstract

The aim of this study was to investigate the efficacy of dietary endotoxin binders [bentonite (BEN) and Saccharomyces cerevisiae cell wall (SCW)] on acute-phase protein (APP) response and liver function in cows during the transition period. Twenty-four multiparous Holstein cows were randomly assigned to one of four treatment groups. The experimental groups consisted of (1) the basal diet (BD) + SCW, (2) BD + SCW + BEN, (3) BD + BEN, and (4) BD (control). Blood samples were taken at 1, 3 and 4 weeks before and 1 and 3 weeks after parturition and serum concentrations of non-esterified fatty acids (NEFA), beta-hydroxybutyrate (BHBA), glucose, haptoglobin (Hp), serum amyloid A(SAA), albumin, g-glutamyl transferase (GGT), aspartate aminotransferase (AST), cholesterol, iron, and lipopolysaccharide (LPS) were measured. The concentrations of LPS, SAA, albumin, and Hp in the blood were within reference range at all times. The level of blood LPS was not high enough to initiate an APP response. Mean BHBA concentration was highest at 1 week after calving. For NEFA, the pattern was similar, with a peak at 1 week after calving. Cholesterol concentration was lower in the SCW group, probably due to a lower lipoprotein concentration. Mean AST concentration was highest at 1 week after calving, especially in the SCW + BEN group. The results of a current study showed that, if the carbohydrate level is not high in the diet to cause rumen acidosis, it is not profitable to supplement BEN and SCW for adsorbing endotoxins in the diet, in transition cows.

Key words

Endotoxin binders Dairy cow Acute-phase protein response Liver function Transition period 

Notes

Acknowledgments

This study was financed by Ph.D student project grant by School of Veterinary Medicine, Shiraz University, Shiraz, Iran. The authors would like to thank the Moghufat Malek industry for provision of cows especially Mr.Miri, Mr.Naghavi and Mr.Ershadi.

Compliance with ethical standards

Statement of Animal Rights

All animals were treated in accordance with the regulations on the guidelines of the Iranian Council of Animal Care (1995), and the experiment was approved by the Institutional Animal Care Committee for Animals Used in Research and We further followed the recommendations of European Council Directive (86/609/EC) of November 24, 1986, regarding the standards of protecting animals used for experimental purposes.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. 1.Department of Clinical Sciences, School of Veterinary MedicineShiraz UniversityShirazIran
  2. 2.Department of Animal Science, Faculty of AgricultureFerdowsi University of MashhadMashhadIran
  3. 3.School of Veterinary ScienceMassey UniversityPalmerston NorthNew Zealand

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