In vitro maturation of class I oocytes of bovine during different tropical seasons
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The aims of this study were to determine the effects of seasonality on yields and quality of bovine abattoir-derived oocytes and their in vitro maturation (IVM) competence in Thailand, a tropical country. Ovaries were collected monthly from a slaughterhouse, from January to June 2017, separated into cool (January to February), summer (March to April), and rainy (May to June) seasons. Cumulus oocyte complexes (COCs) were obtained by follicular aspiration, and subsequently, oocyte yield and quality were examined. Selected class I and II COCs were cultured under IVM condition, and their maturation capacity was also evaluated. Results showed that average numbers of COCs and class I COCs per ovary were highest in summer. However, no significant seasonal difference in oocyte maturation rates was found. The higher numbers of COCs and class I COCs in summer might be caused by their follicular development during the cool season, and might be a reason why the pregnancy rate in summer is better than in the rainy season.
KeywordsCattle Cumulus oocyte complexes In vitro maturation Heat stress
The authors thank Mr. Jirapong Punsiri, a staff of the Chiang Mai municipality slaughterhouse, for helping in the ovary collection.
The Royal Golden Jubilee Ph. D. (RGJ-Ph.D) Programme (Grant No. PHD/0068/2557) and Research Center of Producing and Development of Products and Innovations for Animal Health and Production, Chiang Mai University, financially supported this study.
Compliance with ethical standards
The manuscript does not contain clinical studies or patient data.
Conflict of interest
The authors declare that they have no conflict of interest.
- Hammond, A.C., Olson, T.A., Chase Jr., C.C., Bowers, E.J., Randel, R.D., Murphy, C.N., Vogt, D.W., Tewolde, A., 1996. Heat tolerance in two tropically adapted Bos taurus breeds, Senepol and Romosinuano, compared with Brahman, Angus, and Hereford cattle in Florida, Journal of Animal Science, 74, 295–303.CrossRefGoogle Scholar
- McNatty, K.P., Heath, D.A., Lundy, T., Fidler, A.E., Quirke, L., O’Connel, A., Smith, P., Groome, N., Tisdall, D.J., 1999. Control of early ovarian follicular development, Journal of Reproduction and Fertility, Supplement, 54, 3–16.Google Scholar
- Monteiro, F.M., Freitas, E.C., Melo, D.S., Carvalho, L.M., Teixeira, A.B., Coelho, L.A., Trinca, L.A., Barros, C.M., 2007. Resistance of embryos from Bos indicus cattle during early stages of in vitro development to heat shock compared to embryos crossbred from crossbred cattle, Animal Reproduction, 4, 51–58.Google Scholar
- Paula-Lopes, F.F., Chase Jr, C.C., AI-Katanani, Y.M., Krininger III, C.E., Rivera, R.M., Tekin, S., Majewski, A.C., Ocon, O.M., Olson, T.A., Hansen, P.J., 2003. Genetic divergence in cellular resistance to heat shock in cattle: differences between breeds developed in temperate versus hot climates in responses of preimplantation embryos, reproductive tract tissues and lymphocytes to increased culture temperatures, Reproduction, 125, 285–294.CrossRefGoogle Scholar
- Punyapornwithaya, V., Teepatimakorn, S., Kreusukon, K., Suriyasathaporn, W., 2005. The effects of temperature and relative humidity on the conception rate of cattle in smallholder dairy farms in Chiang Mai. Thai Journal of Veterinary Medicine, 35 (4), 73–79.Google Scholar
- Silva, C.F., Sartorelli, E.S., Castilho, A.C.S., Satrapa, R.A., Puelker, R.Z., Razza, E.M., Ticianelli, J.S., Eduardo, H.P., Loureiro, B., Barros, C.M., 2013. Effects of heat stress on development, quality and survival of Bos indicus and Bos taurus embryos produced in vitro, Theriogenology, 79, 351–357.CrossRefGoogle Scholar
- Torres-Júnior, J.D.S., de FA Pires, M., De Sa, W.F., Ferreira, A.D.M., Viana, J.H.M., Camargo, L.S.D.A., Ramos, A.A., Folhadella, I.M., Polisseni, J., De Freitas, C., Clemente, C. A. A., 2008. Effect of maternal heat-stress on follicular growth and oocyte competence in Bos indicus cattle, Theriogenology, 69(2), 155–166.CrossRefGoogle Scholar