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Tropical Animal Health and Production

, Volume 51, Issue 4, pp 939–948 | Cite as

Assessment of risk factors and isolation of Staphylococcus aureus and Escherichia coli from bovine subclinical mastitic milk in and around Gondar, Northwest Ethiopia

  • Amare Bihon
  • Ashenafi Syoum
  • Ayalew AssefaEmail author
Regular Articles
  • 64 Downloads

Abstract

A cross-sectional study with simple random sampling approach was conducted from November 2017 to May 2018 with the objectives of estimating the prevalence of subclinical mastitis with its associated risk factors and to identify and isolate Staphylococcus aureus and Escherichia coli from mastitic milk in and around Gondar town, Ethiopia. The study was conducted on 334 lactating cows selected from 70 smallholder dairy farms. California mastitis test (CMT) and bacteriological culture methods were used as diagnostic tools. From 334 cows examined, 114 (34.1%) and from 1054 quarters examined, 238 (17.8%) were positive for subclinical mastitis using CMT test. Adult and old cows were 3.681 (95% CI = 1.541, 8.788) and 15.044 (95% CI = 3.051, 74.174) times more likely to have a chance of contracting mastitis than young cows respectively. Cow’s having 4–7 calving were 88.9% (OR = 0.111, 95% CI = 0.29, 0.423) less likely to have a chance of contracting mastitis than cows having ≤ 3 calving by keeping another factors constant. Mid-lactating cows were 63.4% (OR = 0.366, 95% CI = 0.150, 0.890) less likely to have a chance of contracting mastitis than cows in the early lactation stage. Crossbreed cows were also 79.1% (OR = 0.209, 95% CI = 0.046–0.948) less likely to have a chance of contracting mastitis than local breed cows. The odds of subclinical mastitis were 21.81 (95% CI = 4.618, 103.002) times higher in cows having tick infestation compared to cows without tick infestation and animals kept in good housing were 23% (OR = 0.73, 95% CI = 0.027, 0.197) less likely to have mastitis than cows kept in poor housing. The likelihood of mastitis occurrence was 3.372 (95% CI = 0.865, 13.141) times higher in cows managed in intensive farming system than semi-intensive farming system. Out of fifty CMT positive milk samples, proportion of Staphylococcus aureus and Escherichia coli was 17 (34%) and 8 (16%) respectively. As a conclusion, subclinical mastitis is the main important health constraints of dairy cows in the study area. Therefore, management, housing, and environmental sanitation should be improved.

Keywords

Bovine Escherichia coli Gondar Risk factors Staphylococcus aureus Subclinical mastitis 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Abebe, R., Hatiya, H., Abera, M., Megersa, B. and Asmare, K. (2016): Bovine mastitis : prevalence, risk factors and isolation of Staphylococcus aureus in dairy herds at Hawassa milk shed, South Ethiopia. BMC Veterinary Research, 12:1–11.CrossRefGoogle Scholar
  2. Abera, M., Demie, B., Aragaw, K., Regassa, F. and Regassa, A. (2016): Isolation and identification of Staphylococcus aureus from bovine mastitic milk and their drug resistance patterns in Adama town, Ethiopia. Journal of Veterinary Medicine and Animal Health, 2:29–34Google Scholar
  3. Abunna, F., Fufa, G., Megersa, B. and Regassa, A. (2013): Bovine Mastitis : Prevalence , Risk Factors and Bacterial Isolation in Small-Holder Dairy Farms in Addis Ababa City, Ethiopia. Global Veterinaria, 10:647–652.Google Scholar
  4. Alemu, S., Tamiru, F., Almaw, G. and Tsega, A. (2013): Study on bovine mastitis and its effect on chemical composition of milk in and around Gondar Town. Journal of Veterinary Medicine and Animal Health, 5:215–221.Google Scholar
  5. Awale, M., Dudhatra, G., Avinash K., Chauhan, B., Kamani, D., Modi, C., Patel, H. and Mody, S. (2012): Bovine mastitis: A threat to the economy, 1:295.Google Scholar
  6. Bangar, Y. C., & Singh, B. (2014). A systematic review and meta-analysis of prevalence of subclinical mastitis in dairy cows in India. Trop Anim Health Prod.  https://doi.org/10.1007/s11250-014-0718-y
  7. Biressaw, S. and Deme, T. (2015): Prevalence of bovine mastitis and determinant of risk factors in lemu Bilbilo District, Arsi Zone: A cross-sectional study. Global Journal of Veterinary Medicine and Research, 3:80–85.Google Scholar
  8. Birhanu, M., Leta, S., Mamo, G. and Tesfaye, S. (2017): Prevalence of bovine subclinical mastitis and isolation of its major causes in Bishoftu. BMC Research Notes, 10:1–6.CrossRefGoogle Scholar
  9. Bradley, A. (2002): Bovine Mastitis: an Evolving disease. Veterinary Journal, 164:116–128.CrossRefGoogle Scholar
  10. Central Statistical Agency (CSA), (2010): Livestock and Livestock Characteristics, Agricultural Sample Survey. Statistician Bulletin, 2(468): 107.Google Scholar
  11. Central Statistical Authority (CSA), (2014): Report on livestock and livestock characteristics. VolumeII Addis Ababa, Ethiopia.Google Scholar
  12. Dego, K. and Tareke, F. (2003):Bovine mastitis in selected areas of southern Ethiopia. Tropical Animal Health and Production, 35:197–205.CrossRefGoogle Scholar
  13. Demelash, B., Debela, E. and Beyene, F. (2005): Prevalence and Risk Factors of Mastitis in Lactating Dairy Cows in Southern Ethiopia. International Journal of Applied Research in Veterinary Medicine, 3:189–198.Google Scholar
  14. Eriskine, R. (2001): Intramuscular administration of ceftiofursodiu versus intrammamary infusion of penicillin/novobiocin for treatment of Streptococcus agalactiaemastitis in dairy cows. Journal of American Veterinary Medicine Association, 208:258–260.Google Scholar
  15. Food and Agricultural Organization (FAO), (2003): The Technology of Traditional Milk Production in Developing Country. Animal Production and Health Paper, 85: 9–24.Google Scholar
  16. Food and Agricultural Organization (FAO), (2014): Impact of mastitis in small scale dairy production systems. Animal production and healthworking Paper.No. 13. Rome.Google Scholar
  17. Gebrekrustos, M., Aferaa, B. and Tassew, H. (2012): Prevalence of mastitis and its relationship with risk factors in smallholder dairy farms in and around Mekelle. Revista electronica de veterinaria, 13:41.Google Scholar
  18. Getaneh, G. (2016): Prevalence of coagulase negative staphylococcus in mastitis infection in dairy cattle in and around Bahir Dar. World Journal of Pharmaceutical and Medical Research, 2:17–23.Google Scholar
  19. Getaneh, A. M., & Gebremedhin, E. Z. (2017). Meta-analysis of the prevalence of mastitis and associated risk factors in dairy cattle in Ethiopia. Tropical Animal Health and Production, 49(4), 697–705.  https://doi.org/10.1007/s11250-017-1246-3 CrossRefGoogle Scholar
  20. Girma, S., Mammo, A., Bogele, K., Sori, T., Tadesse, F. and Jibat, T. (2012): Study on prevalence of bovine mastitis and its major causative agents in West Harerghe zone, Doba district, Ethiopia. Journal of Veterinary Medicine and Animal Health, 4:116–123.Google Scholar
  21. Hameed, S., Arshad, M., Ashraf, M., Avais, M. and Shahid, M. (2012): Cross-sectional epidemiological studies on mastitis in cattle and buffaloes of Tehsil Burewala, Pakistan. The Journal of Animal and Plant Sciences,22:371–376.Google Scholar
  22. Islam, A., Islam, Z., Islam, A., Rahman, S., and Islam, T. (2011): Prevalence of subclinical mastitis in dairy cows in selected areas of Bangladesh. Bangladesh Journal of Veterinary Medicine, 9:73–78.CrossRefGoogle Scholar
  23. Jarassaeng, C., Aiumlamai, S., Wachirapakorn, C., Techakumphu, M., Noordhuizen, J., Beynen, A. and Suadsong, S. (2012): Risk factors of subclinical mastitis in small holder dairy cows in Khon Kaen province. Thai Journal of Veterinary Medicine, 42:143–151.Google Scholar
  24. Joshi, S. and Gokhale, S. (2006): Status of mastitis as an emerging disease in improved and periurban dairy farms in India. Annals of the New York Academy of Sciences, 1081:74–83).CrossRefGoogle Scholar
  25. Lakew, M., Tolosa, T. and Tigre, W. (2009): Prevalence and major bacterial causes of bovine mastitis in Asella, South Eastern Ethiopia. Tropical Animal Health and Production, 41: 1525–1530.CrossRefGoogle Scholar
  26. Marama, A., Mamu, G. and Birhanu, T. (2016): Prevalence and Antibiotic Resistance of Staphylococcus aureus Mastitis in Holeta Area, Western Ethiopia. Global Veterinaria, 16:365–370.Google Scholar
  27. Mekonnen, H., Workineh, S., Bayleyegne, M., Moges, A. and Tadele, K. (2005): Antimicrobial susceptibility profile of mastitis isolates from cows in three major Ethiopian dairies. MedVet, 176:391–394.Google Scholar
  28. Moges, N., Asfaw, Y. and Belihu, K. (2011): A Cross Sectional Study on the Prevalence of Sub Clinical Mastitis and Associated Risk Factors in and Aronund Gondar, Northern Ethiopia. International Journal of Animal and Veterinary Advances, 3:455–459.Google Scholar
  29. Moges, N., Hailemariam, T., Fentahun, T., Chanie, M.and Melaku, A. (2012). Bovine Mastitis and Associated Risk Factors in Small Holder Lactating Dairy Farms in Hawassa, Southern Ethiopia. Global Veterinaria, 9:441–446.Google Scholar
  30. Mohamed, A., Simeon, E. and Yemesrach, A. (2004): Dairy development in Ethiopia. International Food Policy Research Institute, EPTD Discussion Paper No. 123. Washington, DC, U.S.A.Google Scholar
  31. Bitew, M., Arega, T. and Tadele, T. (2010): Study on bovine mastitis in dairy farms of bahirdar and its environs. Journal of Animal and Veterinary Advances, 9:2912–2917.CrossRefGoogle Scholar
  32. Mungube, E., Tenhagen, B., Regassa, F., Kyule, M., Greiner, M. and Baumann, M. (2004): Risk factors for dairy cow mastitis in the central highlands of Ethiopia. Tropical Animal Health and Production, 36:463–472.CrossRefGoogle Scholar
  33. National Mastitis Council (NMC) (2004): Microbiological procedures for the diagnosis of udder infection, 3rd ed., National Mastitis Council Inc. Arlington, VA.Google Scholar
  34. Ojo, O., Oyekunle, M., Ogunleye, A. and Otesile, E.(2009). Escherichia coli. O157: H7 in Food animals in part of south-western Nigeria: Prevalence and in vitro antimicrobial susceptibility. Tropical Veterinaria, 26:23–30.Google Scholar
  35. Quinn, P., Carter M., Markey, B. and Carter, G., (2002): Clinical Veterinary Microbiology. Harcourt Publishers, Virginia. pp. 331–344.Google Scholar
  36. Radostits, O., Gay, C., Blood, D. and Hinchcliff, K. (2007): A Text Book of the Disease of Cattle, Sheep, Pigs, Goats and Horses. 9th ed. New York: W.B. Sounders Company Ltd. pp 809–827.Google Scholar
  37. Rahman, M., Bhuiyan, M., Kamal, M. and Shamsuddin, M. (2009): Prevalence and risk factors of mastitis in dairy cows. Bangladesh Veterinarian, 26:54–60.CrossRefGoogle Scholar
  38. Sharma, N., Singh, N. and Bhadwal, M. (2011): Relationship of somatic cell count andmastitis: An overview. Asian-Aust. Journal of Animal Science, 24:429–438.Google Scholar
  39. Shiferaw J. and Telila I. (2017): Prevalence of Bovine Mastitis and Assessment of Risk Factors in and Around Wolayta Sodo, Ethiopia. International Journal of Homeopathy and Natural Medicines, 2(1)1–7.Google Scholar
  40. Singh, B.(2006): Mastitis. Teaching materials, Faculty of veterinary medicine, Addis Ababa University, Debrezeit.Google Scholar
  41. Sori, H., Zerinhum, A. and Abdicho, S. (2005): Dairy cattle mastitis in and around Sebeta, Ethiopia. International Journal of Applied Research in Veterinary Medicine, 3:332–338.Google Scholar
  42. Tekle, Y. and Berihe, T. (2016): Bovine mastitis: prevalence, risk factors and major pathogens in the Sidamo Zone SNNPRS, Ethiopia. European Journal of Biology and Medical Science Research, 4:27–43.Google Scholar
  43. Thrusfied, M. (2005): Veterinary epidemiology, 3rd ed, Black Well Science Ltd., Oxford, UK.Google Scholar
  44. Tilahun, A. and Aylate, A. (2015): Prevalence of Bovine Mastitis in Lactating Cows and its Public Health Implications in Selected Commercial Dairy Farms of Addis Ababa,Global Journal of Medical Research, 15:16–24.Google Scholar
  45. Tuke, M., Kassaye, D., Muktar, Y., Negese, T. and Nigusu, K. (2017): Bovine Mastitis : Prevalence and Associated Risk Factors in Alage ATVET College Dairy Farm, Southern Ethiopia. Journal of Veterinary Science and Technology, 8:462.CrossRefGoogle Scholar
  46. Zadok, R, and Schukken, Y. (2006): Use of molecular epidemiology in veterinary practice. Veterinary Clinics of North America: Food Animal Practice, 22(1):229–261.Google Scholar
  47. Zenebe, N., Habtamu, T. and Endale, B. (2014): Study on bovine mastitis and associated risk factors in Adigrat, Northern Ethiopia. African Journal of Microbiology Research, 8, 327–331.CrossRefGoogle Scholar
  48. Zeryehun, T. and Abera, G. (2017): Prevalence and Bacterial Isolates of Mastitis in Dairy Farms in Selected Districts of Eastern Harrarghe Zone, Eastern Ethiopia. Journal of Veterinary Medicine, 2017: 1–6.Google Scholar
  49. Zeryehun, T., Aya, T. and Bayecha, R. (2013): Study on prevalence , bacterial pathogens and associated risk factors of bovine mastitis in small holder dairy farms in and around Addis Ababa , Ethiopia. The Journal of Animal and Plant Sciences, 23:50–55.Google Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.College of Veterinary MedicineSamara UniversitySamaraEthiopia
  2. 2.Sekota Dryland Agricultural Research CenterSekotaEthiopia

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