Tropical Animal Health and Production

, Volume 51, Issue 4, pp 993–995 | Cite as

Vaginal biota number is smaller in tailed than docked hair ewes (Ovis aries), but is not affected by copulation

  • A. OrihuelaEmail author
  • A. Fierros-García
  • C. Hallal-Calleros
  • S. R. Robles-Castro
  • R. Ungerfeld
Short Communications


A greater accumulation of feces could facilitate the entrance of bacteria or inert particles into the vagina, particularly while mating tailed ewes. Two experiments were performed to determine if the tail has an active role in the growth of bacterial flora and the entrance of inert material in the vagina after mating, during induced or natural estrus. Twenty ewes were used, 10 tailed and 10 docked. All ewes displayed estrus after CIDR treatment, and half of them; 5 tailed and 5 docked were exposed to rams during the first experiment. For the second experiment, the rest of the animals were mated 15–17 d later at spontaneous estrus. In the induced estrus, no differences in the number of CFU/mL in docked or tailed ewes were observed before mating, but after mating, CFU values tended to be greater in docked than tailed ewes, and the number of inert particles was greater in docked than tailed ewes (5.8 ± 1.3 vs 1.6 ± 0.6, P = 0.024). During natural estrus, docked ewes had less CFU/mL than tailed ewes (19.6 × 103 ± 0.4 vs 80.0 × 103 ± 0.0, P = 0.006) before mating, and a similar tendency was maintained after mating, when the number of inert particles was similar in docked and tailed ewes. Staphylococcus and Escherichia were observed in the induced estrus, while in the natural estrus, even though these were the more frequent, five other genera of bacteria were observed. It was concluded that the presence of the tail protects the vaginal environment against increases of the biota load.


Welfare, estrus Sheep Mating Contamination Feces 


Compliance with ethical standards

Ethical statement

The study was approved by the Internal Committee for the Care and Use of Experimental Animals at the Universidad Autónoma del Estado de Morelos, México (UAEM), in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments. The manuscript does not contain clinical studies or patient data.


  1. Al-Hilli, Z.B., Ajeel, H.H., 2015. Isolation and identification of bacterial flora from vagina in normal ewes (slaughter and living ewes). Journal of Pharmaceutical Biology Science, 10, 1–4Google Scholar
  2. Fierros-García, A., Ungerfeld, R., Aguirre, V., Orihuela, A., 2018. The tail in tropical hair ewes (Ovis aries) that are in estrus is used as a proceptive signal and favors ram’ copulation. Animal Reproduction Science, 195, 65–70CrossRefGoogle Scholar
  3. Gatti, M., Zunino, P., Ungerfeld, R., 2011. Changes in the aerobic bacterial mucous load after treatment with intravaginal sponges in anoestrous ewes: effect of medroxiprogesterone acetate and antibiotic treatment use. Reproduction in Domestic Animals, 46, 205–208CrossRefGoogle Scholar
  4. Gower, D.B., Holland, K.T., Mallet, A.I., Rennie, P.J., Watkins, W.J., 1994. Comparison of 16-androstene steroid concentrations in sterile apocrine sweat and axillary secretions: interconversions of 16-androstenes by the axillary microflora—a mechanism for axillary odour production in man? Journal of Steroid Biochemistry, and Molecular Biology, 48, 409–418Google Scholar
  5. Manes, J., Hozbor, F., Alberio, R., Ungerfeld, R., 2014. Intravaginal placebo sponges affect negatively the conception rate in sheep. Small Ruminant Research, 120, 108–111CrossRefGoogle Scholar
  6. Manes, J., Fiorentino, M.A., San Martino, S., Ungerfeld, R., 2018. Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle. Livestock Science 208, 55–59CrossRefGoogle Scholar
  7. Orihuela, A., Ungerfeld, R., Fierros-García, A., Pedernera, M., Aguirre, V., 2018. Rams prefer tailed than docked ewes as sexual partners. Reproduction in Domestic Animals, in press.Google Scholar
  8. Parr, E.L., Schaedler, R.W., Hirsch, J.G., 1967. The relationship of polymorphonuclear leukocytes to infertility in uteri containing foreign bodies. Journal of Experimental Medicine, 126, 523–537CrossRefGoogle Scholar
  9. Suárez, G., Zunino, P., Carol, H., Ungerfeld, R., 2006. Changes in the aerobic vaginal bacterial mucous load and assessment of the susceptibility to antibiotics after treatment with intravaginal sponges in anestrous ewes. Small Ruminant Research, 63, 39–43CrossRefGoogle Scholar
  10. Ungerfeld, R., Silva, L., 2005. The presence of normal vaginal flora is necessary for normal sexual attractiveness of estrous ewes. Applied Animal Behaviour Science, 93, 245–250CrossRefGoogle Scholar
  11. Weinhold, L.L., Ingersoll, D.W., 1988. Modulation of male mouse genital sniff, attack, and mount behaviors by urogenital substances from estrous females. Behavioral and Neural Biology, 50, 207–228CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Facultad de Ciencias Agropecuarias de la Universidad Autónoma del Estado de MorelosCuernavacaMéxico
  2. 2.Unidad de Medicina Familiar No 37, del Instituto Mexicano del Seguro SocialLos MochisMéxico
  3. 3.Departamento de Fisiología, Facultad de VeterinariaUniversidad de la RepúblicaMontevideoUruguay

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