Tropical Animal Health and Production

, Volume 51, Issue 3, pp 523–528 | Cite as

Effects of replacing rice bran with tamarind seed meal in concentrate mixture diets on the changes in ruminal ecology and feed utilization of dairy steers

  • Ahkarapon Nunoi
  • Metha WanapatEmail author
  • Suban Foiklang
  • Thiwakorn Ampapon
  • Bounnaxay Viennasay
Regular Articles


Feed ingredients costs have been impacting the production cost. Attempts have been made to use local feed resources in order to reduce feed costs. The objective of this study was to identify effect of using crushed tamarind seed meal (TSM) in concentrate mixture on rumen fermentation, dry matter intake, and digestibility of dairy steers. Four rumen-fistulated dairy steers were used in a 4 × 4 Latin Square Design. Four levels of TSM were used to replace rice bran (RB) in the concentrate mixtures in four treatments (T1 = 0% replacement of RB, T2 = 30% replacement of RB, T3 = 60% replacement of RB, T4 = 100% replacement of RB). The findings revealed that replacement of TSM for RB resulted in similar digestibility of nutrients and intakes (P > 0.05). However, rumen fermentation parameters were remarkably improved, namely total VFA and the concentration of C3 especially at the highest level of replacements (100%, T4), (P < 0.05). Rumen protozoal population was found lowered in all replacements, especially those in higher levels of TSM replacement. Consequently, the rumen methane productions were significantly reduced. TSM can be a promising energy source to replace rice bran, hence lowering the cost of concentrate mixture.


Tamarind seed meal Rumen fermentation Feed resource 



The authors would like to express their most sincere gratitude to the Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University and the Thailand Research Fund (TRF) through the International Research Network (IRN) program (TRF-IRN57W0002) and TRF-IRG598001 and tamarind processing group of Koksa-ngud village for their financial support and research facilities. The Post Doc. Training Program provided by the Research and Graduate School, Khon Kean University, Thailand (Grant no.58440), is also acknowledged.

Funding information

This study received financial support from the Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University and the Thailand Research Fund (TRF) through the International Research Network (IRN) program (TRF-IRN57W0002) and TRF-IRG598001 and tamarind processing group of Koksa-ngud village.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Informed consent

Informed consent was obtained from all individual participants included in this study.


  1. Ampapon, T., Wanapat, M. and Kang, S.C., 2015. Rumen metabolism of swamp buffaloes fed rice straw supplemented with cassava hay and urea. Trop. Anim. Health and Prod. 48, 779–784.CrossRefGoogle Scholar
  2. Anantasook, N., Wanapat, M. and Cherdthong, A., 2014. Manipulation of ruminal fermentation and methane production by supplementation of rain tree pod meal containing tannins and saponins in growing dairy steers. J. Animal Physiol. and Anim. Nutr. 98(1), 50–55.CrossRefGoogle Scholar
  3. AOAC( Official Methods of Analysis). 2012., Association of Official Analytical Chemists, 19th ed. Gaithersburg, MD.Google Scholar
  4. Bhatta, R., Krishnamoorthy, U. and Mohammed, F., 2000. Effect of feeding tamarind (Tamarindus indica) seed husk as a source of tannin on dry matter intake, digestibility of nutrients and production performance of crossbred dairy cows in mid-lactation. J. Anim. Feed Sci. and Tech., 82, 67–74.CrossRefGoogle Scholar
  5. Bhatta, R., Krishnamoorthy, U. and Mohammed F., 2001. Effect of tamarind (Tamarindus indica) seed husk tannins on in vitro rumen fermentation. J. Anim. Feed Sci. and Tech., 90, 143–152.CrossRefGoogle Scholar
  6. Cieslak, A., Zmora, P., Matkowski, A., Nawrot-Hadzik, I., Pers-Kamczyc, E., El-Sherbiny, M., Bryszak M. and Szumacher-Strabel, M., 2016. Tannins from Sanguisorba offiinalis affect in vitro rumen methane production and fermentation .J. Anim. and Plant Sci., 26(1), 54–62Google Scholar
  7. Crocker, C.L., 1967. Rapid determination of urea nitrogen in serum or plasma without deproteinization. The American J. Med. Tech., 33(5), 361–365.Google Scholar
  8. Foiklang, S., Wanapat, M. and Norrapoke, T., 2016a. Effect of grape pomace powder, mangosteen peel powder and monensin on nutrient digestibility, rumen fermentation, nitrogen balance and microbial protein synthesis in dairy steers. Asian–Australas. J. Anim. Sci., 29(10), 1416–1423.PubMedGoogle Scholar
  9. Foiklang, S., Wanapat, M. and Norrapoke, T., 2016b. In vitro rumen fermentation and digestibility of buffaloes as influenced by grape pomace powder and urea treated rice straw supplementation. Anim. Sci. J. 87(3), 370–377.CrossRefPubMedGoogle Scholar
  10. Galyean, M., 1989. Laboratory Procedure in Animal Nutrition Research. Dept. of Animal and Range Science, New Mexico State University, USA.Google Scholar
  11. Gemeda, B.S. and Hassen, A., 2015. Effect of Tannin and Species Variation on in vitro digestibility, gas, and methane production of tropical browse plants. Asian– Australas. J. Anim. Sci., 28, 188–199.CrossRefPubMedPubMedCentralGoogle Scholar
  12. Hristov, A.N., Oh, J. and Firkins, J.L., 2013. Mitigation of methane and nitrous oxide emissions from animal operations: I. A review of enteric methane mitigation options. J. Sci., 91, 5045–5069.Google Scholar
  13. IPCC, 2007. Climate Change 2007: Synthesis Report. Contribution of Working Groups I, II and III to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change [Core Writing Team, Pachauri, R.K and Reisinger, A. (eds.)]. IPCC, Geneva, Switzerland, 104 pp.Google Scholar
  14. Jayanegara, A., Wina, E. and Takahashi, J., 2014. Meta-analysis on methane mitigating properties of saponin-rich sources in the rumen: Influence of addition levels and plant sources. Asian–Australas. J. Anim. Sci., 27, 1426–1435.PubMedPubMedCentralGoogle Scholar
  15. Kamra, D.N., Agarwal, N. and Chaudhary, L.C. 2006. Inhibition of ruminal methanogenesis by tropical plants containing secondary compounds. Int. Congr., 1293, 156–163.CrossRefGoogle Scholar
  16. Kara, K., Güçlü, B.K. and Baytok, E., 2015. Comparison of nutrient composition and antib methanogenic properties of different Rosaceae species. J.Anim. Feed Sci. and Tech., 24, 308–314.CrossRefGoogle Scholar
  17. Marangoni, A., Alli, I. and Kermasha, S., 1988. Composition and properties of seeds of the tree legume Tamarindus indica. J. Food Sci., 35, 1452–1455.CrossRefGoogle Scholar
  18. Moate, P.J., Williams, S.R.O., Torok, V.A., Hannah, M.C., Ribaux, B.E., Tavendale, M.H.,Eckard, R.J., Jacobs, J.L., Auldist, M.J. and Wales, W.J., 2014. Grape marc reduce methane emissions when fed to dairy cows. J. Dairy Sci. 97, 5073–5087.CrossRefPubMedGoogle Scholar
  19. Moss, R., Jouany, I.P. and Newbold, J., 2000. Methane production by ruminants: its contribution to global warming. In Ann. de Zootech., 49(9), 231–253.CrossRefGoogle Scholar
  20. Naumann, H.D., Tedeschi, L.O., Zeller, W.E. and Huntley N.F., 2017. The role of condensed tannins in ruminant animal production: advances, limitations and future directions. R. Bras. Zootec., 46(12), 929–949.CrossRefGoogle Scholar
  21. Pilajun, R. and Wanapat, M., 2013. Microbial population in the rumen of swamp buffalo (Bubalus bubalis) as influenced by coconut oil and mangosteen peel supplementation. J. Anim. Physiol. and Anim. Nutr., 97, 439–445.CrossRefGoogle Scholar
  22. Samuel, M., Sagathevan, S., Thomas, J. and Mathen, G., 1997. An HPLC method for estimation of volatile fatty acids in ruminal fluid. Ind. J. Dairy Sci., 67, 805–807.Google Scholar
  23. SAS, 2013. User’s Guide: Statistic, Version 9.4th Edition. SAS Inst. Inc., Cary, NC, USA..Google Scholar
  24. Souza, C.M., Oliveira, R.L., Voltolini, T.V., Menezes, D.R., dos Santos, N.J.A., Barbosa, A.M., Silva, T.M., Pereira, E.S. and Bezerra, L.R., 2018., Lambs fed cassava silage with added tamarind residue: Silage quality, intake, digestibility, nitrogen balance, growth performance and carcass quality. J. Anim. Feed Sci. and Tech., 235, 50–59.CrossRefGoogle Scholar
  25. Steel, R.G.D. and Torrie, J.H., 1980. Principles and Procedures of Statistics: A Biometrical Approach, 2nd ed. McGraw-Hill Book Co. NY, USA.Google Scholar
  26. Steinfeld, H., Gerber, P., Wassenaar, T., Castel, V., Rosales, M. and de Haan, C., 2006. Livestock’s role in climate change and air pollution. 79-123. In: Livestock’s long shadow: environmental issues and options (eds. Steinfeld, H., P. Gerber, T. Wassenaar, V. Castel, M. Rosales, and C. de Haan), FAO, Rome, Italy.Google Scholar
  27. Van Soest, P.J., Robertson, J.B. and Lewis, B.A., 1991. Methods for dietary fiber neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74, 3583–3597.CrossRefPubMedGoogle Scholar
  28. Wanapat, M., 1990. Nutritional Aspects of Ruminant Production in Southeast Asia with Special Reference to Thailand. Dept. of Animal Science, Faculty of Agriculture, Khon Kaen Univ., Khon Kaen. pp. 217.Google Scholar
  29. Wanapat, M. and Pimpa, O., 1999. Effect of ruminal NH3-N levels ruminal fermentation, purine derivatives, digestibility and rice straw intake in swamp buffaloes. Asian– Australas. J. Anim. Sci., 12, 904–907.CrossRefGoogle Scholar
  30. Wanapat, M., Gunun, P., Anantasook, N. and Kang, S., 2014. Changes of rumen pH, fermentation and microbial population as influenced by different ratios of roughage (rice straw) to concentrate in dairy steers. J. Agric. Sci., 152(4), 675–685.CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Ahkarapon Nunoi
    • 1
  • Metha Wanapat
    • 1
    Email author
  • Suban Foiklang
    • 2
  • Thiwakorn Ampapon
    • 1
  • Bounnaxay Viennasay
    • 1
  1. 1.Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of AgricultureKhon Kaen UniversityKhon KaenThailand
  2. 2.Faculty of Animal Science and TechnologyMaejo UniversityChiang MaiThailand

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