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Tropical Animal Health and Production

, Volume 51, Issue 2, pp 481–485 | Cite as

Short communication: detection and molecular characterization of hepatitis E virus in domestic animals of São Tomé and Príncipe

  • João Rodrigo MesquitaEmail author
  • Claudia Istrate
  • Nânci L. Santos-Ferreira
  • Ana S. Ferreira
  • Joana Abreu-Silva
  • José Veiga
  • Wim H. M. van der Poel
  • Maria S. J. Nascimento
Short Communications

Abstract

As in most of the African continent, the status of hepatitis E virus (HEV) infection in domestic animals in São Tomé and Príncipe, an archipelago off the western equatorial coast of Central Africa, is also completely unknown. In the present study, we investigated the presence of HEV among domestic animals in São Tomé and Príncipe. A total of 93 stool samples from different animal species (goat, cow, pig, chicken, duck, and monkey) were tested for HEV RNA using two real-time RT-PCR assays, followed by a nested RT-PCR assay for sequencing and phylogenetic analysis. A total of six samples (1 cow stool and 5 pig stools) were found to be positive for HEV RNA of which one pig stool was positive by broad spectrum nested RT-PCR. Phylogenetic analysis showed that the retrieved sequence clustered within HEV subgenotype 3f, similar to zoonotic strains of European countries and posing interesting questions on past introduction of European HEV into São Tomé and Príncipe archipelago. This is the first report describing the presence and molecular characterization of HEV in São Tomé and Príncipe.

Keywords

Hepatitis E virus São Tomé e Príncipe Domestic animals Zoonoses 

Notes

Acknowledgements

We acknowledge the support of the Ministry of Health of São Tomé e Príncipe and the representatives of the non-governmental organization Instituto Marquês de Valle Flor, Dr. Edgar Neves, Dr. António Lima (in STP), and Dr. Ahmed Zaky (in Lisbon) for guaranteeing the local logistic as well as the Calouste Gulbenkian Foundation in Lisbon, Portugal, to contribute to the project consumables.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Adelabu, O. A., Chuks Iweriebor, B., Nwodo, U. U., Obi, L. C., Okoh, A. I., 2017. Incidence and Molecular Characterization of Hepatitis E Virus from Swine in Eastern Cape, South Africa. Advances in Virology, 1073253.Google Scholar
  2. Adlhoch, C., Wolf, A., Meisel, H., Kaiser, M., Ellerbrok, H., Pauli, G., 2009. High HEV presence in four different wild boar populations in East and West Germany. Veterinary Microbiology, 139: 270–8.CrossRefGoogle Scholar
  3. Adlhoch, C,, Avellon, A., Baylis, S. A., Ciccaglione, A. R., Couturier, E., de Sousa, R., Epštein, J., Ethelberg, S., Faber, M., Fehér, Á., Ijaz, S., Lange, H., Manďáková, Z., Mellou, K., Mozalevskis, A., Rimhanen-Finne, R., Rizzi, V., Said, B., Sundqvist, L., Thornton, L., Tosti, M. E., van Pelt, W., Aspinall, E., Domanovic, D., Severi, E., Takkinen, J., Dalton, H. R. 2016. Hepatitis E virus: Assessment of the epidemiological situation in humans in Europe, 2014/15. Journal of Clinical Virology, 82: 9–16.CrossRefGoogle Scholar
  4. Baylis, S. A., Hanschmann, K. M., Blumel, J., Nubling, C. M. 2011. Standardization of hepatitis E virus (HEV) nucleic acid amplification technique-based assays: an initial study to evaluate a panel of HEV strains and investigate laboratory performance. Journal of Clinical Microbiology, 49: 1234–1239.CrossRefGoogle Scholar
  5. Berto, A., Baker, J. A., Mesquita, J. R., Nascimento, M. S. J., Banks, M., Martelli, F., Ostanello, F., Angeloni, G., Ruggeri, F. M., di Bartolo, I., Vasickova, P., Diez-Valcarce, M., Hernandez, M., Lazaro, R. D., van der Poel, W. H. M., 2012a. Prevalence and transmission of hepatitis E virus in domestic swine populations in different European countries. BMC Research Notes, 5: 190.CrossRefGoogle Scholar
  6. Berto, A., Mesquita, J. R., Hakze-van der Honing, R., Nascimento, M. S., van der Poel, W. H., 2012b. Detection and characterization of hepatitis E virus in domestic pigs of different ages in Portugal. Zoonoses and Public Health, 59: 477–481.CrossRefGoogle Scholar
  7. Doceul, V., Bagdassarian, E., Demange, A., Pavio, N., 2016. Zoonotic Hepatitis E Virus: Classification, Animal Reservoirs and Transmission Routes. Viruses, 8: 270.CrossRefGoogle Scholar
  8. Epelboin, L., Nicand, E., Roussin, C., Lernout, T., Pettinelli, M. E., Tesse, S., Ali, R., Aubry, P., 2011. A sporadic case of genotype 3f acute hepatitis E in Mayotte. Médecine et Maladies Infectieuses, 41: 392–394.CrossRefGoogle Scholar
  9. Haider, N., Khan, M. S. U., Hossain, M. B., Sazzad, H. M. S., Rahman, M. Z., Ahmed, F., Zeidner, N. S., 2017. Serological evidence of hepatitis E virus infection in pigs and jaundice among pig handlers in Bangladesh. Zoonoses and Public Health, 64: 572–577CrossRefGoogle Scholar
  10. Huang, F., Li, Y., Yu, W., Jing, S., Wang, J., Long, F., He, Z., Yang, C., Bi, Y., Cao, W., Liu, C., Hua, X., Pan, Q.,2016. Excretion of infectious hepatitis E virus into milk in cows imposes high risks of zoonosis. Hepatology, 64: 350–9.CrossRefGoogle Scholar
  11. Johne, R., Plenge-Bönig, A., Hess, M., Ulrich, R. G., Reetz, J., Schielke, A., 2010. Detection of a novel hepatitis E-like virus in faeces of wild rats using a nested broad-spectrum RT-PCR. Journal of General Virology, 91: 750–758.CrossRefGoogle Scholar
  12. Jothikumar, N., Cromeans, T.L., Robertson, B.H., Meng, X.J., Hill, V.R., 2006. A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus. Journal of Virological Methods, 131:65–71.CrossRefGoogle Scholar
  13. Kaba, M., Colson, P., Musongela, J. P., Tshilolo, L., Davoust, B., 2010. Detection of hepatitis E virus of genotype 3 in a farm pig in Kinshasa (Democratic Republic of the Congo). Infection, Genetics and Evolution, 10: 154–7.CrossRefGoogle Scholar
  14. Kamar, N., Bendall, R., Legrand-Abravanel, F., Xia, N. S., Ijaz, S., Izopet, J., Dalton, H. R., 2012. Hepatitis E. Lancet, 379, 2477–88.CrossRefGoogle Scholar
  15. Kamel, A. H., Ali, M. A., El-Nady, H. G., Deraz, A., Aho, S., Pothier, P., Belliot, G., 2011. Presence of enteric hepatitis viruses in the sewage and population of Greater Cairo. Clinical Microbiology and Infection, 17: 1182–1185.CrossRefGoogle Scholar
  16. Kim, J. H., Nelson, K. E., Panzner, U., Kasture, Y., Labrique, A. B., Wierzba, T. F., 2014. A systematic review of the epidemiology of hepatitis E virus in Africa. BMC Infectious Diseases, 14: 308.CrossRefGoogle Scholar
  17. Legrand-Abravanel, F., Mansuy, J. M., Dubois, M., Kamar, N., Peron, J. M., Rostaing, L., Izopet, J., 2009. Hepatitis E virus genotype 3 diversity, France. Emerging Infectious Diseases, 15: 110–4.CrossRefGoogle Scholar
  18. Mesquita, J. R., Oliveira, R. M., Coelho, C., Vieira-Pinto, M., Nascimento, M. S., 2016. Hepatitis E Virus in Sylvatic and Captive Wild Boar from Portugal. Transboundary and. Emerging Diseases, 63(5):574–8.CrossRefGoogle Scholar
  19. Mokhtari, C., Marchadier, E., Haïm-Boukobza, S., Jeblaoui, A., Tessé, S., Savary, J., Roque-Afonso, A. M. 2013. Comparison of real-time RT-PCR assays for hepatitis E virus RNA detection. Journal of Clinical Virology, 58: 36–40.CrossRefGoogle Scholar
  20. Owolodun, O. A., Gerber, P. F., Giménez-Lirola, L. G., Kwaga, J. K., Opriessnig, T., 2014. First report of hepatitis E virus circulation in domestic pigs in Nigeria. American Journal of Tropical Medicine and Hygiene, 91: 699–704.CrossRefGoogle Scholar
  21. de Paula SV., Wiele, W., Mbunkah, A. H., Daniel, A. M., Kingsley, M. T., Schmidt-Chanasit, J., 2013. Hepatitis E virus genotype 3 strains in domestic pigs, Cameroon. Emerging Infectious Diseases, 19: 666–8.Google Scholar
  22. Purcell, R. H., Emerson, S.U., 2010. Hidden Danger: The Raw Facts about Hepatitis E Virus. Journal of Infectious Diseases, 202: 819–821.CrossRefGoogle Scholar
  23. Rolfe, K.J., Curran, M.D., Mangrolia, N., Gelson, W., Alexander, G.J., L’estrange, M., Vivek, R., Tedder, R., Ijaz, S., 2010. First case of genotype 4 human hepatitis E virus infection acquired in India. Journal of Clinical Virology, 48:58–61.CrossRefGoogle Scholar
  24. Smith, D. B., Simmonds, P., International Committee on Taxonomy of Viruses Hepeviridae Study Group, Jameel, S., Emerson, S. U., Harrison, T. J., Meng, X. J., Okamoto, H., Van der Poel, W. H., Purdy, M. A., 2014. Consensus proposals for classification of the family Hepeviridae. Journal of General Virology, 95: 2223–2232.CrossRefGoogle Scholar
  25. Thom, K., Gilhooly, P., McGowan, K., Malloy, K., Jarvis, L. M., Crossan, C., Scobie, L., Blatchford, O., Smith-Palmer, A., Donnelly, M. C., Davidson, J. S., Johannessen, I., Simpson, K. J., Dalton, H. R., Petrik, J. 2018. Hepatitis E virus (HEV) in Scotland: evidence of recent increase in viral circulation in humans. Euro Surveillance, 23(12).Google Scholar
  26. Traoré, K. A., Ouoba, J. B., Huot, N., Rogée, S., Dumarest, M., Traoré, A. S., Pavio, N., Barro, N., Roques, P., 2015. Hepatitis E Virus Exposure is Increased in Pork Butchers from Burkina Faso. Journal of Tropical Medicine and Hygiene, 93:1356–9CrossRefGoogle Scholar
  27. Ward, P., Poitras, E., Leblanc, D., Letellier, A., Brassard, J., Plante, D., Houde, A. 2009. Comparative analysis of different TaqMan real-time RT-PCR assays for the detection of swine Hepatitis E virus and integration of Feline calicivirus as internal control. Journal of Applied Microbiology, 106: 1360–1369.CrossRefGoogle Scholar
  28. WHO. Hepatitis E, 2015. Available from: http://www.who.int/mediacentre/factsheets/fs280/en/
  29. Zeng, M. Y., Gao, H., Yan, X. X., Qu, W. J., Sun, Y. K., Fu, G. W., Yan, Y. L., 2017. High hepatitis E virus antibody positive rates in dogs and humans exposed to dogs in the south-west of China. Zoonoses and Public Health, 64: 684–688CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • João Rodrigo Mesquita
    • 1
    • 2
    Email author
  • Claudia Istrate
    • 3
  • Nânci L. Santos-Ferreira
    • 4
  • Ana S. Ferreira
    • 4
  • Joana Abreu-Silva
    • 4
  • José Veiga
    • 5
    • 6
  • Wim H. M. van der Poel
    • 7
  • Maria S. J. Nascimento
    • 2
    • 4
  1. 1.Escola Superior Agrária de Viseu (ESAV), Centro de Estudos em Educação, Tecnologias e Saúde (CI&DETS)Instituto Politécnico de ViseuViseuPortugal
  2. 2.Epidemiology Research Unit (EPIUnit), Institute of Public HealthUniversity of PortoPortoPortugal
  3. 3.Global Health and Tropical Medicine (GHTM), Unidade de Microbiologia Médica, Instituto de Higiene e Medicina Tropical (IHMT)Universidade NOVA de Lisboa (UNL)LisbonPortugal
  4. 4.Laboratório de MicrobiologiaFaculdade de Farmácia da Universidade do PortoPortoPortugal
  5. 5.Instituto Marquês de Valle FlorSão ToméSão Tomé and Príncipe
  6. 6.Ministério da SaúdeSão ToméSão Tomé and Príncipe
  7. 7.Wageningen Bioveterinary ResearchLelystadThe Netherlands

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