Tropical Animal Health and Production

, Volume 50, Issue 4, pp 831–836 | Cite as

Epidemiological investigation of Borrelia burgdorferi in horses in the municipality of Sinop—MT, Brazil

  • Suyane Nayara Garcia Socoloski
  • Bruno Gomes de Castro
  • Matheus Dias Cordeiro
  • Adivaldo Henrique da Fonseca
  • Marcio Barizon Cepeda
  • Rafael Romero Nicolino
  • Luciano Bastos Lopes
Regular Articles

Abstract

Borrelia burgdorferi sensu stricto is the main etiological agent of Lyme disease (LD) in the USA. In Brazil, it is believed that a similar spirochete is the causal agent of the Baggio–Yoshinari syndrome (BYS), a zoonosis also transmitted by ticks, whose clinical manifestations are similar to those of LD. Despite the epidemiological importance, there are no studies reporting the presence and the prevalence of B. burgdorferi among horses in Mato Grosso State. The aim of this study was to detect and measure the frequency of IgG antibodies anti-B. burgdorferi American strain G39/40 in horses in the municipality of Sinop, MT—Brazil, using the indirect enzyme-linked immunosorbent assay (ELISA) for serological diagnosis. Blood samples from 367 horses were collected in 81 farms. An epidemiological questionnaire was applied during the visits to obtain information related to the animals and the farms. From the 367 horses, 214 were positive for B. burgdorferi sensu stricto according to the results of the ELISA test, representing an apparent prevalence of 54.04% [CI = 0.4548051–0.6237234]. Concomitantly, 89 blood samples were taken for molecular analysis by nested polymerase chain reaction (PCR). According to the PCR test results, none of the samples were reactive, although 53 of these samples were reactive according to ELISA. Seventy five farms (92.59%) had at least one reactive horse for B. burgdorferi. Our results support the hypothesis of the presence of anti-Borrelia spp. antibodies in horses in Mato Grosso, reaching a high animal prevalence. Besides that, leisure/sport purposes proved to be a risk factor, with an odds ratio of 3.16. These findings clearly indicate the need of borreliosis control in Sinop and make a significant contribution to the knowledge of the disease in Mato Grosso.

Keywords

Horse Borreliosis Prevalence ELISA 

Notes

Acknowledgements

Special thanks to the Coordination for the Improvement of Higher Education Personnel | CAPES, in reason for the scholarship.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Statement of animal rights

The authors whose names are listed above certify that they have not committed any willful act of abuse or cruelty or neglected animal rights included on this study. Besides, all the procedures recommended by OIE to apply the diagnosis test were used as described in methodology.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Suyane Nayara Garcia Socoloski
    • 1
  • Bruno Gomes de Castro
    • 2
  • Matheus Dias Cordeiro
    • 3
  • Adivaldo Henrique da Fonseca
    • 3
  • Marcio Barizon Cepeda
    • 3
  • Rafael Romero Nicolino
    • 4
  • Luciano Bastos Lopes
    • 5
  1. 1.Animal ScienceFederal University of Mato Grosso-UFMTSinopBrazil
  2. 2.Laboratory of Infectious DiseasesFederal University of Mato Grosso–UFMTSinopBrazil
  3. 3.Laboratory of ParasitologyFederal Rural University of Rio de Janeiro–UFRRJSeropédicaBrazil
  4. 4.Federal University of Jequitinhonha and Mucuri Valleys-UFVJM, Agronomic & Animal Sciences InstituteUnaíBrazil
  5. 5.Embrapa AgrossilvipastorilSinopBrazil

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