Transgenic Research

, Volume 22, Issue 6, pp 1241–1247 | Cite as

Striatum-specific expression of Cre recombinase using the Gpr88 promoter in mice

  • Chihiro HisatsuneEmail author
  • Naoko Ogawa
  • Katsuhiko MikoshibaEmail author
Brief Communication


We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked chloramphenicol acetyltransferase gene, and examined the Cre activity in the Tg mouse brains by assessing β-galactosidase activity. Cre activity was specifically detected in the caudate-putamen, nucleus accumbens, and olfactory tubercle of the Gpr88-Cre Tg mouse brain. Medium spiny neurons within the caudate-putamen exhibited Cre activity. Thus, Gpr88-Cre Tg mice could be a useful tool for analyzing the function of the basal ganglia by using Cre/loxP systems.


Transgenic mice Bacterial artificial clone LacZ Basal ganglia 



We thank CHRCO for a BAC clone (RP23-99k15), and Dr. Jun-ichi Miyazaki for providing the CAG-CAT-Z Tg mice. We also thank Dr. Shigeyoshi Itohara and Dr. Yoshikazu Saito (Lab. for Behavioral Genetics, RIKEN) for the NLS-Cre-poly(A) cassette and technical advice and help, and Dr. Takashi Arai (Support unit for Animal Resources Development, RIKEN) for generation of Tg mice. We also thank all member of our laboratory, especially Dr. Katsuhiro Kawaai, Dr. Takeyuki Sugawara, and Ms. Etsuko Ebisui for their kind help. Supported by The Moritani Scholarship Foundation (C. H), JSPS KAKENHI Grant Numbers, 20500301 (C. H), and 20220007 (K. M) and The Japan Science and Technology Agency (K. M).

Conflict of interest

The authors have no conflicts of interest to declare.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Laboratory for Developmental NeurobiologyRIKEN Brain Science Institute (BSI) 2-1 HirosawaWako CityJapan
  2. 2.Calcium Oscillation Project, ICORP-SORSTJapan Science and Technology Agency (JST)KawaguchiJapan

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