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Ruthenium hydrazone complexes with 1:1 and 1:2 metal–ligand stoichiometry: a comparison of biomolecular interactions and in vitro cytotoxicities

  • Jayanthi Eswaran
  • Neethu K. Sankar
  • Nattamai S. P. Bhuvanesh
  • Kaveri M. Velusamy
Article
  • 33 Downloads

Abstract

Two ruthenium(II) complexes [RuIICl(PPh3)2(L)] (1) and [RuII(L)2] (2) were synthesized by reacting [RuCl2(PPh3)3] and thiophene-2-carboxylic acid (1-pyridine-2-yl-ethylidene)-hydrazide (HL) in methanol–chloroform, and characterized by elemental analysis and spectral and XRD data. The ratio of ligand to metal is 1:1 in the former complex and 2:1 in the latter. Interaction of these complexes with CT-DNA was studied using absorption and emission spectral studies; these show that both the complexes interact with CT-DNA through intercalative modes of interaction. Their BSA-binding activity results indicated the operation of static quenching mechanism and stronger binding of tryptophan residues than tyrosine residues. In vitro cytotoxicity assays against HeLa and MCF-7 cell lines showed better activity of both the complexes compared to the standard drug cisplatin. Overall, the activity of complex 2 with two units of coordinated ligand showed better activity than the other one.

Graphical abstract

Notes

Acknowledgements

We would like to acknowledge University Grants Commission (UGC) for the award of Basic Scientific Research fellowship to one of the authors (S. N) (Grant No. F.25-1/2014-15(BSR)7-26/2007/(BSR); dt: 05.11.2015).

Supplementary material

11243_2018_303_MOESM1_ESM.docx (77 kb)
Supplementary material 1 (DOCX 77 kb)

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Jayanthi Eswaran
    • 1
  • Neethu K. Sankar
    • 2
  • Nattamai S. P. Bhuvanesh
    • 3
  • Kaveri M. Velusamy
    • 2
  1. 1.Department of ChemistryKongunadu Arts and Science CollegeCoimbatoreIndia
  2. 2.Inorganic Chemistry Research Laboratory, Department of ChemistryBharathiar UniversityCoimbatoreIndia
  3. 3.Department of ChemistryTexas A&M UniversityCollege StationUSA

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