Towards artificial seeds from microspore derived embryos of Brassica napus
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Microspore derived embryos are haploid and their immediate diploidization generates doubled haploid homozygous plants, whereas a normal breeding process would take 7–8 generations to attain homozygosity. However, the flexibility available in conventional seeds—storage, transport, variable planting time, and handling—is not possible with microspore derived embryos in breeding programs; they are continuously growing from induction of embryogenesis to planting in the soil, without a pause. Artificial seed technology can by-pass the expensive and time-consuming process of acclimatizing and loss of in vitro derived embryos in the green house. The doubled haploid technology in Brassica species has advanced considerably on many fronts—reliable induction of embryogenesis, in vitro diploidization, desiccation and conversion of embryos to plantlets. Although microspore derived embryos are bipolar, they are not considered within the scheme of artificial seeds. The development of artificial seeds in brassica, however, needs the input from other biotechnologies to develop vigorous embryos capable of conversion to plants. It is now necessary for empirical research on microspore derived embryos of Brassica to expand the applications available to the plant breeder by including the biotechnologies of encapsulation and embryo priming to develop artificial seeds. The objective of this review is to draw the attention of researchers to make the transition from microspore-derived embryos to artificial seeds in Brassica crop species, by bringing together relevant studies from the relevant biotechnologies. This would expand the present scope of artificial seeds and thus provide more options and flexibility to the Brassica plant breeder.
Artificial seeds from microspore derived embryos enhance breeding of Brassica species.
KeywordsArtificial seeds Microspore-derived embryos Conversion of embryos Brassica species Encapsulation Artificial endosperm Priming embryos
Indole butyric acid
Murashige and Skoog medium
Naphthalene acetic acid
MCMI wishes to thank the Deutscher Akademischer Austausch Dienst (DAAD), Germany, and the Erasmus Mundus Experts Sustain programme for research fellowships, and the National Science Foundation of Sri Lanka for a travel award.
The review was conceived by both authors. MCMI wrote the manuscript with inputs from CM who also revised the drafts. Both authors read and approved the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
- Ara H, Jaiswal U, Jaiswal VS (2000) Synthetic seed: prospects and limitations. Curr, SciGoogle Scholar
- Baillie AMR, Epp DJ, Hutcheson D, Keller WA (1992) In vitro culture of isolated microspores and regeneration of plants in Brassica campestris. Plant Cell Rep 11:234–237Google Scholar
- Cangahuala-Inocente GC, Dal Vesco LL, Steinmacher D et al (2007) Improvements in somatic embryogenesis protocol in Feijoa (Acca sellowiana (Berg) Burret): induction, conversion and synthetic seeds. Sci Hortic (Amsterdam) 111:228–234. https://doi.org/10.1016/j.scienta.2006.10.030 CrossRefGoogle Scholar
- Chen ZZ, Snyder S, Fan ZG, Loh WH (1994) Efficient production of doubled haploid plants through chromosome doubling of isolated microspores in Brassica napus. Plant Breed 113:217–221. https://doi.org/10.1111/j.1439-0523.1994.tb00725.x CrossRefGoogle Scholar
- Gu HH, Zhou WJ, Hagberg P (2003) High frequency spontaneous production of doubled haploid plants in microspore cultures of Brassica rapa ssp. chinensis. Euphytica 134:239–245. https://doi.org/10.1023/b:euph.0000004945.01455.6d CrossRefGoogle Scholar
- Haddadi P, Moieni A, Karimzadeh G, Abdollahi MR (2008) Effects of gibberellin, abscisic acid and embryo desiccation on normal plantlet regeneration, secondary embryogenesis and callogenesis in microspore culture of Brassica napus L. Int J Plant Prod 2:153–162Google Scholar
- Hilhorst HW, Finch-Savage WE, Buitink J, Bolingue W, Leubner-Metzger G (2010) Dormancy in plant seeds. In: Dormancy and resistance in harsh environments. Springer, pp 43–67Google Scholar
- Kermode AR (1995) Regulatory mechanisms in the transition from seed development to germination: interactions between the embryo and the seed environment. Seed Dev Germination 41:273Google Scholar
- Lambardi M, Benelli C, Ozudogru EA, Ozden-Tokatli Y (2006) Synthetic seed technology in ornamental plants. Floric Ornam plant Biotechnol Adv Top Issues 2:347–354Google Scholar
- McDonald MB (2000) Seed priming. In: Black M, Bewley JD (eds) Seed technology and its biological basis. Sheffield Academic Press, Sheffield, pp 287–325Google Scholar
- Murashige T (1977) Plant cell and organ cultures as horticultural practices. Symp Tissue Cult Horticult Purp 78:17–30Google Scholar
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x CrossRefGoogle Scholar
- Prem D, Solís MT, Bárány I et al (2012) A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus. BMC Plant Biol. https://doi.org/10.1186/1471-2229-12-127 Google Scholar
- Redenbaugh K (1993) Hydrated coatings for synthetic seeds. In: Synseeds-applications of synthetic seeds to crop improvement. CRC Press, pp 35–46Google Scholar
- Rudolf K, Bohanec B, Hansen M (1999) Microspore culture of white cabbage, Brassica oleracea var. capitata L.: genetic improvement of non-responsive cultivars and effect of genome doubling agents. Plant Breed 118:237–241. https://doi.org/10.1046/j.1439-0523.1999.118003237.x CrossRefGoogle Scholar
- Siong PK, Mohajer S, Taha RM (2012) Production of artificial seeds derived from encapsulated in vitro micro shoots of cauliflower, Brassica oleracea var. Botrytis. Rom Biotechnol Lett 17:7549–7556Google Scholar
- Swamy MK, Balasubramanya S, Anuradha M (2009) Germplasm conservation of patchouli (Pogostemon cablin Benth.) by encapsulation of in vitro derived nodal segments. Int J Biodivers Conserv 8:224–230Google Scholar
- Tian H, Yao CY, Sun MX (2004) High frequency conversion of microspore-derived embryos of Brassica napus cv. Topas by supplemental calcium and vitamins. Dev Biol 76:159–165Google Scholar
- Zhang Y, Wang A, Liu Y et al (2011) Effects of the antiauxin PCIB on microspore embryogenesis and plant regeneration in Brassica rapa. Sci Hortic (Amsterdam) 130:32–37Google Scholar