Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 139, Issue 3, pp 605–608 | Cite as

Plant regeneration in Ixora chinensis from young leaves

  • TingGe Li
  • Hong Cai
  • TongXin Wang
  • YingGe Fu
  • WenHan Yang
  • AnJin Zhao
  • Zheng Cui
  • Jian WangEmail author
Research Note


Ixora chinensis Lam. is a widespread tropical woody ornamental plant for its abundant and bright flowers. In this study, we established a regeneration system of I. chinensis for the first time using young leaves as explants on the Murashige and Skoog (MS) medium supplemented with different Plant Growth Regulators (PGRs). The optimal conditions for shoot regeneration include 4.0 mg/L 6-Benzylaminopurine (6-BA), 1.0 mg/L Thidiazuron (TDZ) and 1.0 mg/L α-Naphthalene acetic acid (NAA) with a shoot regeneration rate of 23.7%. Subculture of shoots on MS medium fortified with 1.0 mg/L 6-BA and 0.5 mg/L NAA achieved a survival ratio of 80.8% and rooting ratio of 30.5%. This result will be helpful in gene biotechnology to develop more colorful or fragrant flowers of I. chinensis in the future.

Key message

This study is the first report to establish a regeneration system for Ixora chinensis.


Chinese Ixora Regeneration Tender leaves Callus induction Shoot induction 



This research was supported by the Open Foundation of National Forest Genetic Resource Platform (Grant No. HD-zkpt-2018001).

Author contribution

W.J. designed the research; L.TG., C.H. completed the whole experiment and analyzed the data; L.TG. wrote the paper; F.YG., Y.WH., Z.AJ., C.Z. assisted in revising the paper; W.J., W.TX. made the final revision to the paper.

Compliance with ethical standards

Conflict of interest

Authors do not have any conflict of interests.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Key Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Key Laboratory of Hainan Province for Biology of Tropical Ornamental Plants GermplasmHainan UniversityHaikouChina
  2. 2.College of ForestryHainan UniversityHaikouChina

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