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Stress associated protein 1 regulates the development of glandular trichomes in Artemisia annua

  • Yuting Wang
  • Xueqing Fu
  • Lihui Xie
  • Wei Qin
  • Ling Li
  • Xiaofen Sun
  • Shihai XingEmail author
  • Kexuan TangEmail author
Original Article
  • 37 Downloads

Abstract

Artemisinin, a sesquiterpene lactone endoperoxide extracted from the aerial part of Artemisia annua L., is widely known as the useful treatment for malaria. Increasing the density of the glandular trichomes in A. annua is an effective method for increasing the production of artemisinin. Here, we identified a transcription factor, AaSAP1 containing A20/AN1 zinc finger motif, which encodes stress associated protein 1 (SAP1). The expression analysis in various tissues indicated that AaSAP1 predominately expressed in the trichomes. Methyl jasmonate, abscisic acid and gibberellic acid induced the expression of AaSAP1. Notably, up-regulation or down-regulation of the transcriptional level of AaSAP1 led to an increase or decrease in the density of the glandular trichomes of A. annua, respectively. In addition, overexpression of AaSAP1 significantly enhanced the content of artemisinin in A. annua. Our results reveal that AaSAP1 positively regulates the development of the glandular trichomes, and it is a valuable gene in genetic engineering of A. annua for increasing the production of artemisinin.

Key message

AaSAP1 positively regulates the development of the glandular trichomes and the production of artemisinin in A. annua.

Keywords

Artemisia annua Stress associated protein Glandular trichome Artemisinin 

Notes

Acknowledgements

This research was funded by Grants from the Bill & Melinda Gates Foundation (OPP1199872); the National Science Foundation of China (18Z103150043); China National Key Research and Development Program (2017ZX09101002-003-002).

Author contributions

YW and KT conceived and designed the project. YW, XF, LX and WQ conducted the experiments. YW, FX, LL and XS analyzed the data. YW and XF drafted the paper. KT and SX reviewed the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human or animal rights

This article does not contain any studies with human or animal subjects performed by the any of the authors.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Joint International Research Laboratory of Metabolic & Developmental Sciences, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D CenterShanghai Jiao Tong UniversityShanghaiChina
  2. 2.College of PharmacyAnhui University of Chinese MedicineHefeiChina

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