Sodium nitroprusside enhances callus induction and shoot regeneration in high value medicinal plant Canscora diffusa
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The present investigation was carried out to demonstrate the plant morphogenetic potential of sodium nitroprusside (SNP) in a commercially important medicinal plant Canscora diffusa. Leaf and nodal explants were used and highest callusing frequency (97.18%) was recorded for leaf explants supplemented with 1.0 mg/L NAA +2.0 mg/L 2ip + 15 µM SNP. SNP enhanced multiple shoot formation in both nodal and leaf explants derived callus considerably in the media supplemented with 1.0 mg/L NAA + 15 µM SNP and 0.5 mg/L BAP + 15 µM SNP (98.32 and 96.12%) respectively. The root induction also drastically increased with the supplementation of SNP (10 µM) in combination with 1.0 mg/L IBA. Our results proved that SNP with other growth hormones acts synergistically to enhance the in vitro responses including the callus, shoot, and root induction. This optimized tissue culture system will be very useful for the mass propagation of C. diffusa for both commercial and conservation aspects.
First report using sodium nitroprusside to enhance in vitro propogation of Canscora diffusa presented. Synergestic action of various growth regulators on SNP supplementation reported. Leaf and nodal segments used as explants produced better callusing and higher shoot formation with SNP treatments.
KeywordsSodium nitroprusside Canscora diffusa Plant growth regulators Mass multiplication
Dr. S. Sivakumar thanks National Post-Doctoral Fellowship (Sanction Order No: PDF/2016/002258), Science and Engineering Research Board, Department of Science and Technology, Government of India. Mr. S. Sathish acknowledges ICMR, New Delhi (No.3/1/2/102/2018-Nut.) for fellowship support. We would also like to thank, University Grants Commission-Special Assistance Programme (UGC-SAP) and Department of Science and Technology-Fund for Improvement of S&T Infrastructure in Higher Educational Institutions (DST-FIST) for the financial support to carry out this research.
SS and SS designed, executed the experiments and prepared the manuscript. HPS and VR contributed substantially to the research in experimental and statistical analysis. RS mobilized the funds and critically evaluated the manuscript.
Compliance with ethical standards
Conflict of interest
All authors read, approved the manuscript and declare that there is no conflict of interest.
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