Effect of cytokinins and sucrose concentration on the efficiency of micropropagation of ‘Zes006’ Actinidia chinensis var. chinensis, a red-fleshed kiwifruit cultivar

  • H. Saeiahagh
  • M. Mousavi
  • Claudia Wiedow
  • H. B. Bassett
  • R. PathiranaEmail author
Original Article


The effect of N6(3-hydroxybenzyl)adenine (meta-Topolin—mT) was compared with that of N6-benzylaminopurine (BAP) and zeatin at the proliferation stage of micropropagation of red-fleshed Actinidia chinensis var. chinensis ‘Zes006’ in two separate experiments. Shoot number, shoot weight, leaf number and leaf area were significantly higher in mT-supplemented media compared with BAP or zeatin. When transferred to rooting media, plantlets that were propagated in mT-supplemented media readily produced roots, enabling easy acclimation to the greenhouse, whereas none of the plantlets propagated in BAP- or zeatin-supplemented media produced roots. Using 12 pairs of Simple Sequence Repeat primers designed for A. chinensis var. chinensis, a very low rate of somaclonal variation was detected at some loci in plantlets produced in zeatin- (1.04%), BAP- (0.4%) as well as in mT- (0.2%) supplemented media. Overall, mT in equimolar concentrations was the better cytokinin for tissue culture of ‘Zes006’ kiwifruit and may well be applicable to many other kiwifruit genotypes.

Key message

Supplementation of media with meta-Topolin for in vitro propagation of the red-fleshed kiwifruit cultivar ‘Zes006’ enhanced better shoot proliferation, giving healthy plantlets that were easier to acclimatize to the greenhouse environment compared with media supplemented with 6-benzylaminopurine or zeatin. It also induced a lower rate of somaclonal variation, as detected by SSR markers.


Shoot proliferation Acclimation Meta-Topolin Somaclonal variation SSR markers In vitro culture O-glucosylation 



We thank Andrew Mullan and Belinda Diepenheim for media preparation and Tony Corbett for figures. We thank Duncan Hedderley for comments on statistical analysis, and Drs Paul Johnston and Sue Gardiner for useful comments on the manuscript. This work was funded by The New Zealand Institute for Plant and Food Research Limited under the Kiwifruit Royalty Investment Programme.

Author Contributions

RP, HS and CW conceptualised the research and designed the experiments. HS, CW and RP conducted the research. HBB designed the primers described in Table 1. RP managed the project. HS, RP, CW and MM wrote the manuscript. HS conducted the statistical analysis.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest with the contents of this article.

Supplementary material

11240_2019_1597_MOESM1_ESM.pdf (145 kb)
Supplementary material 1 (PDF 144 KB)
11240_2019_1597_MOESM2_ESM.pdf (391 kb)
Supplementary material 2 (PDF 391 KB)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.The New Zealand Institute for Plant and Food Research LimitedPalmerston NorthNew Zealand
  2. 2.Department of Horticultural Science, Faculty of AgricultureShahid Chamran University of AhvazAhvazIran

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