Optimizing culture medium ingredients and micrografting devices can promote in vitro micrografting of cut roses on different rootstocks

  • Mahboubeh Davoudi Pahnekolayi
  • Ali TehranifarEmail author
  • Leila Samiei
  • Mahmoud Shoor
Original Article


Tissue culture and micrografting techniques are known as effective procedures to produce healthy rose rootstocks and high quality scions. In order to establish successful micrografting protocol for roses, several factors including different concentrations of Benzylamino-purine (BA) (0, 1, 1.5, 2 mg l−1) + 0.1 mg l−1 α-Naphthaleneacetic acid (NAA), sucrose concentrations (30, 50, 70 g l−1), types of grafting devices (aluminium foil, parafilm, paper bridge), and types of rose scions cultivars (Rosa hybrida cv. Red One, R. hybrida cv. Samurai) on two different types of native (Rosa canina) and commercial rose rootstocks (Rosa multiflora cv. Natal Briar) were examined. The high micrografting success (100%) was micrografting R. hybrida cv. Samurai scion on R. mutiflora cv. Natal Briar rootstock with the paper bridge as the best grafting device in the liquid Van der salm (VS) medium containing 2 mg l−1 BA + 0.1 mg l−1 NAA + 300 mg l−1 Casein Hydrolyzate + 2 g l−1 myo-inositol + 50 g l−1 sucrose. Treating the wounded explants (scions and rootstocks) with silver nitrate (50 mg l−1) during the micrografting process, increased the survival of micrografts. The micrografted plants were successfully rooted and well acclimatized. The present results provide the first efficient protocol to produce high successful micrografted rose plantlets in tissue culture.

Key message

It is the first research protocol for micrografting of native and commercial roses by considering important factors relating to successful micrografting such as plant growth regulators, growth adjuvants, grafting devices, and medium ingredients.


Culture medium ingredients Micrografting Paper bridge Rootstock Scion 





Casein hydrolyzate


Completely randomized design


Ethylenediamine di-2-hydroxy-phenylacetic acid


Ethylenediaminetetraacetic acid


Murashige and Skoog


α-Naphthaleneacetic acid


Plant growth regulators




Van der Salm



This research was financially supported by Ferdowi University of Mashhad, Mashhad, Iran (Grant No. 38934).

Author contributions

This research is part of a Phd thesis. MDP designed and carried out the experiments, analyzed the data and wrote the manuscript under the supervision of AT, LS and MS. Furthermore, they evaluated the original and final version of the paper. All the authors edited and approved the final manuscript.

Compliance with ethical standards

Conflict to interest

The authors confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.


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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Horticultural Sciences, Ornamental Plants, Department of Horticulture, College of AgricultureFerdowsi University of MashhadMashhadIran
  2. 2.Department of Horticultural Science and LandscapeFerdowsi University of MashhadMashhadIran
  3. 3.Department of Ornamental Plants, Research Center for Plant SciencesFerdowsi University of MashhadMashhadIran

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