Identification of somatic embryogenesis (SE) related proteins through label-free shotgun proteomic method and cellular role in Catharanthus roseus (L.) G. Don
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Somatic embryogenesis (SE) is an intricate in vitro multi-step biotechnological tool used to develop embryos/plants from a single or a group of somatic cells. It is a model technique for understanding various plant developmental pathways. A lot of research is going on to elucidate the mechanism underlying the process of SE. This study was aimed at the identification of SE related proteins in a medicinally important plant, Catharanthus roseus via label free liquid chromatography–mass spectroscopy (LC–MS). LC–MS is a sensitive and reliable technique than the gel based techniques, using LC–MSMS in tandem for separation and identification of proteins. Here, we are reporting for the first time SE related proteins in C. roseus by using gel free shotgun proteomic approach. The non embryogenic and embryogenic calli of C. roseus were used for comparative proteome analysis. A total of 3573 proteins were identified in both embryogenic and non embryogenic calli of which 1511 proteins were found to be common in both the calli. In non embryogenic callus 982 proteins while in embryogenic callus 1079 proteins were exclusively identified, which were associated with varied cellular functions. The most of these proteins function in different metabolic processes and stress responses. More than 72 stress responsive proteins and isoforms were observed exclusively in embryogenic callus including glutathione S transferase, ascorbate peroxidase, catalase, superoxide dismutase, alkylhydro peroxidase, SOD Fe N domain containing protein, pyridine nucleotide disulphide oxidoreductase, thioredoxin reductase. The role of plant growth regulators (PGRs) in inducing stress cause switching on/off of several genes has been discussed, led biochemical and molecular alterations in acquiring somatic embryogenic competence.
Proteomic map of Catharanthus roseus was prepared. A total of 3573 proteins were identified, of which 1079 were embryogenic. These proteins have role in metabolic and stress responses.
KeywordsEmbryogenic-non embryogenic callus Gel-free proteomics Somatic embryogenesis Catharanthus roseus
The first author is thankful to University Grant Commission (UGC) for providing financial assistance with (Grant No. 2061530497). The authors are also thankful to the Department of Botany, Jamia Hamdard, and University of Delhi for providing laboratory and other research facilities for this research.
BG, AF, NZ conducted most of the experiments. BG also made the draft of article. AM and MVR edited the article.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest in this research.
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