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Tissue-specific accumulation and subcellular localization of chalcone isomerase (CHI) in grapevine

  • Huiling Wang
  • Wei Wang
  • Jicheng Zhan
  • Weidong HuangEmail author
  • Haiying XuEmail author
Original Article
  • 43 Downloads

Abstract

Flavonoids are widely distributed secondary metabolic products with many biological functions in plants. Further elucidation of the accumulation and localization patterns of its biosynthesis enzymes will broaden our understanding of flavonoids biosynthesis and regulation. Chalcone isomerase (CHI, EC 5.5.1.6) is an early-step enzyme in the flavonoids biosynthesis pathway. In this study, using an antibody specifically developed against grapevine CHI enzyme, we found that the accumulation of CHI protein exhibited temporal and spatial specificity. In grape berries, CHI was investigated mainly in the outer hypodermis cells of exocarp tissues, in the vascular bundles of mesocarp; and in the integument and the cells around the raphe of seeds. At the subcellular level, CHI was visualized in the cytoplasm, nucleus, and plastids (chloroplasts) of the exocarp cells, while only in the cytoplasm of mesocarp vascular bundle cells. In grapevine vegetable organs, the leaf mesophyll and phloem of leaf veins, the pith ray and primary phloem of stems, the primary phloem and endoderm of roots, and the young leaves, leaf primordium, and the growth point of leaf buds were CHI signal-positive. In these tissue cells, CHI was primarily observed in the cytoplasm, cell wall, and nucleus. The distinct localization patterns of CHI suggested the complexity of flavonoids biosynthesis in grapevine.

Key Message

The distribution of CHI protein exhibited temporal and spatial specificity. Different subcellular localization patterns were observed in grape berries and grapevine vegetable tissues.

Keywords

Grapevine Chalcone isomerase Flavonoids Immunolocalization 

Abbreviations

PAL

Phenylalanine ammonia-lyase

C4H

Cinnamate 4-hydroxylase

CHI

Chalcone isomerase

CHS

Chalcone synthase

ANS

Anthocyanidin synthase

IgG

Immunoglobulin fractions

BSA

Bovine serum albumin

PBS

Phosphate-buffered saline

BS

Blocking solution

RSR

Regular salt rinse solution

DFAB

Days after full bloom

Notes

Acknowledgements

This study was funded by the special fund of China Agriculture Research System (CARS-29), the National Natural Science Foundation of China (No. 31601712), Beijing Municipal Natural Science Foundation (No. 6182007), and the Natural Science Foundation of Beijing Academy of Agriculture and Forestry Science (No. QNJJ201604).

Author contributions

Weidong Huang and Haiying Xu designed the experiments and revised the manuscript; Huiling Wang, Wei Wang, Jicheng Zhan performed the experiments; Huiling Wang and Wei Wang processed statistical analysis; Huiling Wang prepared the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Beijing Academy of Forestry and Pomology SciencesBeijingChina
  2. 2.Key Laboratory of Silviculture of the State Forestry Administration, The Institute of ForestryThe Chinese Academy of ForestryBeijingChina
  3. 3.Key Laboratory of Urban Agriculture (North China)Ministry of AgricultureShanghaiChina
  4. 4.College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingChina
  5. 5.Beijing Engineering Research Center for Deciduous Fruit TreesBeijingChina

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