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Elicitors derived from endophytic fungi Chaetomium globosum and Paraconiothyrium brasiliense enhance paclitaxel production in Corylus avellana cell suspension culture

  • Mina Salehi
  • Ahmad Moieni
  • Naser Safaie
  • Siamak Farhadi
Original Article
  • 62 Downloads

Abstract

Paclitaxel is an impressive chemotherapeutic agent that shows biological activity against a wide range of cancers. Cell suspension culture of Corylus avellana has been reported as an alternative source for production of the valuable secondary metabolite, paclitaxel. The fungal elicitors have been considered as the most impressive strategy for enhancement of secondary metabolites in plant cell culture. In this study, two endophytic fungal strains YEF20 and HEF114 were isolated from Taxus baccata and C. avellana, respectively. The isolates YEF20 and HEF114 were identified as Chaetomium globosum and Paraconiothyrium brasiliense, respectively by sequencing of ITS1-5.8S-ITS2 rDNA region. This is the first report of P. brasiliense on C. avellana tree. Also, this study presents the positive effect of fungal elicitors on paclitaxel production in C. avellana for the first time. The effect of fungal elicitors on paclitaxel production was dependent on fungal species, and also type, concentration and adding time of elicitors to cell culture. The highest total yield of paclitaxel in cell culture treated with cell extract (CE) of C. globosum (291.5 µg L−1) was obtained by using 10% (v/v) of this fungal elicitor on 17th day of cell culture cycle (late log-phase), which was about 4.1 times obtained in the control culture. The cell culture treated with C. globosum CE had an average growth rate of 0.491 g L−1 day−1, i.e. 12.3% lower than that in the control. Among the fungal elicitors, 10% (v/v) CE of C. globosum on 17th day of culture cycle showed the best results with respect to extracellular paclitaxel portion (44.0%). Paclitaxel secretion to culture medium is essential for large-scale steady production of paclitaxel.

Keywords

Endophytic fungi Taxus baccata Anticancer Secondary metabolite 

Abbreviations

CSC

Cell suspension cultures

CE

Cell extract

CF

Culture filtrate

DW

Dry weight

FW

Fresh weight

HPLC

High performance liquid chromatography

MS

Murashige and Skoog (1962)

PDA

Potato Dextrose Agar

PDB

Potato Dextrose Broth (PDB)

ITS

Internal transcribed spacer

Notes

Acknowledgements

Authors gratefully acknowledge Research Deputy of Tarbiat Modares University, Tehran for financial support of this research project.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval and informed consent

All authors approve ethics and consent for participation and publication.

Supplementary material

11240_2018_1503_MOESM1_ESM.docx (705 kb)
Supplementary material 1 (DOCX 704 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Mina Salehi
    • 1
  • Ahmad Moieni
    • 1
  • Naser Safaie
    • 2
  • Siamak Farhadi
    • 1
  1. 1.Department of Plant Breeding and Biotechnology, Faculty of AgricultureTarbiat Modares UniversityTehranIran
  2. 2.Department of Plant Pathology, Faculty of AgricultureTarbiat Modares UniversityTehranIran

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