Morphogenetic potential of different sources of explants for efficient in vitro regeneration of Genipa sp.
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Genipa americana L. is a species with high economic potential with considerable promise in the fruit, medicinal and agroindustrial fields. The in vitro cultivation of genipapo is an alternative that may overcome the difficulties imposed by the traditional propagation system, allowing the multiplication of plants on a large scale and using technologies in conservation programs and to improve phytopharmaceutical production. In this context, the aim of this work was to evaluate the morphogenetic potentials of different sources of explants to establish an efficient system for in vitro regeneration of G. americana. For callus induction, shoots differentiation and further plant regeneration, segments of hypocotyl, root and leaf from in vitro established seedlings were used. The explants were inoculated in MS medium supplemented with 6-benzylaminopurine (BAP) at concentrations of 0.0, 1.12, 2.25 and 3.37 mg L−1. The morphogenetic pattern and regeneration capacity showed correlations with the explant source and BAP concentration. MS medium supplemented with 1.12 mg L−1 BAP proved to be optimum for adventitious shoots induction in segments hypocotyl. It was possible to obtain a efficient protocol for the in vitro regeneration of G. americana that allowed high shoot regeneration rates (80%) using hypocotyl segments with low concentrations of BAP (1.12 mg L−1). The regenerated plantlets showed a high capacity for acclimatization, presenting 90% survival rate 30 days after exposure to the ex vitro conditions.
KeywordsRubiaceae Micropropagation Woody plants Growth regulators Genipapo
The authors are thankful to the National Council for Scientific and Technological Development (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES) and Foundation for Supporting Research of the State of Minas Gerais (FAPEMIG) for granting scholarships.
RRdS, PDdOP and RP conceived and designed the experiment. RRdS, RRdS, MVdR and DPCdS performed the experiments, and analyzed and interpreted the data. RRdS and PDdOP wrote the manuscript. All authors, read and approved the final version of the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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