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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 135, Issue 3, pp 535–544 | Cite as

Molecular cloning of Ve promoters from Gossypium barbadense and G. hirsutum and functional analysis in Verticillium wilt resistance

  • Lixia Liu
  • Weiqiao Wang
  • Jun Yang
  • Yan Zhang
  • Guiyin Zhang
  • Zhiying Ma
  • Xingfen Wang
Original Article
  • 95 Downloads

Abstract

Verticillium wilt caused by Verticillium dahliae is the most serious disease in cotton. Ve gene isolated from Gossypium barbadense is one of the resistant genes related to Verticillium wilt. In this study, we analyzed Ve expression pattern in different resistant cotton cultivars under V. dahliae stress, and isolated the upstream promoters of Ve from G. barbadense and G. hirsutum. We found that the expression of Ve gene in upland cottons increased significantly at 6 hpi and decreased at 12 hpi, however, continued to increase in Sea Island cotton under V. dahliae stress. Through detecting the GUS activity in the transgenic pVe::GUS Arabidopsis, we discovered that the upstream from − 979 to − 1 bp was the important region of Ve gene promoter. The activity of Ve gene promoters was higher in the roots than that in other tissues in Arabidopsis, and the promoters could be induced by V. dahliae. Moreover, the activity of promoter in G. barbadense was significantly higher than that in G. hirsutum, and the promoter of Ve in G. barbadense contained regulatory elements S1FBOXSORPS1L21 and S1FSORPL21 compared to that in G. hirsutum. We inferred that the regulatory elements S1FBOXSORPS1L21 and S1FSORPL21 were related to the Ve gene expression. This provides new insights into dissecting the defense mechanisms of Ve against V. dahliae in cotton.

Keywords

Promoter Ve gene Verticillium wilt G. barbadense G. hirsutum 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31171597) and Hundreds of Innovative Talents Project of Hebei Province (14226308D).

Author contributions

XW designed the research. LL and WW performed the experiments. LL, JY, YZ and GZ performed the data analysis. LL prepared the manuscript. XW and ZM revised the manuscript. All authors approved the final version of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Lixia Liu
    • 1
  • Weiqiao Wang
    • 1
  • Jun Yang
    • 1
  • Yan Zhang
    • 1
  • Guiyin Zhang
    • 1
  • Zhiying Ma
    • 1
  • Xingfen Wang
    • 1
  1. 1.North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Key Laboratory for Crop Germplasm Resources of HebeiHebei Agricultural UniversityBaodingChina

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