High frequency conversion of non-embryogenic synseeds and assessment of genetic stability through ISSR markers in Gymnema sylvestre

  • Taiba Saeed
  • Anwar Shahzad
  • Naseem Ahmad
  • Shahina Parveen
Research Note
  • 62 Downloads

Abstract

The present study describes the first attempt of exploiting encapsulation technology for high plantlet recovery, short-term storage and conservation of Gymnema sylvestre—an antidiabetic liana. Indole-3-butyric acid (IBA) pretreated nodal segments (NS) were encapsulated in sodium alginate (Na2-alginate) matrix and the optimal culture conditions were evaluated in terms of maximum conversion capacity of synseeds into complete plantlets. Highest conversion frequency of synseeds was obtained on Murashige and Skoog’s (MS) medium supplemented with 5.0 µM 6-benzyladenine (BA). Augmentation of Na2-alginate matrix with plant growth regulators (PGRs) and additive further improved in vitro conversion rates and the synthetic endosperm composed of 3% Na2-alginate in MS + 2.5 µM BA + 2.5 µM gibberellic acid (GA3) + 50 µM adenine sulphate (AdS) stimulated maximum recovery (88.2 ± 0.48%) of complete plantlets from synseeds. Studies on short term cold storage of synseeds showed that nutrient encapsulation maintains the viability of NS for a storage period of 8 weeks. Ex-vitro conversion of synseeds was also carried out on soilrite and vermicompost (3:1) mixture under culture room conditions. Monomorphic DNA profiles produced through Inter-Simple Sequence Repeat (ISSR) markers confirmed the genetic uniformity between synseed derived and mother plantlets.

Keywords

Encapsulation Nodal segments Sodium alginate Conversion Synthetic endosperm ISSR markers 

Abbreviations

AdS

Adenine-sulphate

BA

6-Benzyladenine

GA3

Gibberellic acid

IBA

Indole-3-butyric acid

ISSR

Inter simple sequence repeats

MS

Murashige and Skoog’s medium (1962)

PGRs

Plant growth regulators

Notes

Acknowledgements

Taiba Saeed gratefully acknowledges UGC for providing the financial assistance under the scheme of Maulana Azad National Fellowship (File No. MANF-MUS-UTT-2624). Authors are also thankful to DST- SERB, for financial support in the form of Young Scientist Projects (Vide Nos. SB/FT/LS-364/2012 and SB/YS/LS-156/2013).

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Taiba Saeed
    • 1
  • Anwar Shahzad
    • 1
  • Naseem Ahmad
    • 1
  • Shahina Parveen
    • 1
  1. 1.Plant Biotechnology Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia

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