Advertisement

Effect of explant type and genotype on the accumulation of bioactive compounds in adventitious root cultures of Polygonum multiflorum

  • Thanh-Tam Ho
  • Cheol-Seung Jeong
  • Hyoshin Lee
  • So-Young Park
Original Article
  • 59 Downloads

Abstract

The correlation between growth and accumulation of bioactive compounds was investigated in six genotypes of Polygonum multiflorum, grown in vitro, soil-grown (ex vitro), and as adventitious root cultures. Adventitious roots were induced on leaf and root explants from plantlets of the six genotypes grown in vitro. Line PM-06 achieved the highest biomass in vitro (0.91 g plant−1 FW; 0.1 g plant−1 (DW)), whereas line PM-05 produced the highest biomass ex vitro (54.67 g plant−1 FW; 12.93 g plant−1 DW). A comparison of the six lines found that total phenolic content (TPC) and total flavonoid content (TFC) were highest in roots from line PM-01 grown in vitro, but line PM-06 produced the highest levels of bioactive compounds in tubers (99.06 mg g−1 DW TPC; 42.31 mg g−1 DW TFC), which were 2.5-fold, 5.0-fold, and 4.8-fold higher than the highest levels produced by in vitro roots, in vitro shoots, and ex vitro shoots, respectively. Although adventitious root line AR-06 produced the greatest overall biomass (60.12 g L−1 FW; 6.36 g L−1 DW), bioactive content was highest in line AR-01 (50.35 mg g−1 DW TPC; 22.51 mg g−1 TFC). There was a strong correlation between phenolic production in plant roots grown in vitro and adventitious root lines, and plant roots grown ex vitro and adventitious root lines. Adventitious root cultures of P. multiflorum line AR-06 showed great potential for producing phenolic compounds. Such cultures may therefore provide an alternative to naturally grown plants as a potential biomass source for the production of bioactive compounds.

Keywords

Adventitious root culture Genotype Phenolic compounds Polygonum multiflorum Root biomass 

Notes

Acknowledgements

This work was supported by the Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Advanced Production Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Grant Number 315013-4).

Author contributions

T-TH acquired the data and wrote the manuscript. C-SJ and HL participated in data interpretation and revising of the manuscript to include important intellectual content. S-YP made substantial contributions to data interpretation, revising of the manuscript, the conception, and design of this study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

References

  1. Baque MA, Hahn EJ, Paek KY (2010) Induction of adventitious root from leaf explants of Morinda citrifolia as affected by auxin and light quality. In Vitro Cell Dev Biol-Plant 46:71–80CrossRefGoogle Scholar
  2. Baque MA, Moh SH, Lee EJ, Zhong JJ, Paek KY (2012) Production of biomass and useful compounds from adventitious roots of high-value added medicinal plants in bioreactor. Biotechnol Adv 30:1255–1267CrossRefGoogle Scholar
  3. Bounda GA, Feng Y (2015) Review of clinical studies of Polygonum multiflorum Thunb. and its isolated bioactive compounds. Pharm Res 7:225–236Google Scholar
  4. Cui XH, Chakrabarty D, Lee EJ, Paek KY (2010) Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Bioresour Technol 101:4708–4716CrossRefGoogle Scholar
  5. Cui XH, Murthy HN, Paek KY (2014) Pilot-scale culture of Hypericum perforatum L. adventitious roots in airlift bioreactors for the production of bioactive compounds. Appl Biochem Biotechnol 174:784–792CrossRefGoogle Scholar
  6. Folin O, Ciocalteu V (1927) On trysonic and tryptophane determination in protein. J Biol Chem 27:627–650Google Scholar
  7. Gómez-Aguirre YA, Zamilpa A, González-Cortazar M, Trejo-Tapia G (2012) Adventitious root cultures of Castilleja tenuiflora Benth. as a source of phenylethanoid glycosides. Ind Crops Prod 36:188–195CrossRefGoogle Scholar
  8. Han MN, Lu JM, Zhang GY, Yu J, Zhao RH (2015) Mechanistic studies on the use of Polygonum multiflorum for the treatment of hair graying. BioMed Res Int.  https://doi.org/10.1155/2015/651048 Google Scholar
  9. Hatano T, Kagawa H, Yasuhara T, Okuda T (1998) Two new flavonoids and other constituents in licorice: their relative astringency and radical scavenging effects. Chem Pharm Bull 36:2090–2097CrossRefGoogle Scholar
  10. Jiang YJ, Piao XC, Liu JS, Jiang J, Lian ZX, Kim MJ, Lian ML (2015) Bioactive compound production by adventitious root culture of Oplopanax elatus in balloon-type airlift bioreactor systems and bioactivity property. Plant Cell Tissue Organ Cult 123:413–425CrossRefGoogle Scholar
  11. Jiang XL, Piao XC, Gao R, Jin MY, Jiang J, Jin XH, Lian ML (2017) Improvement of bioactive compound accumulation in adventitious root cultures of an endangered plant species, Oplopanax elatus. Acta Physiol Plant 39:226.  https://doi.org/10.1007/s11738-017-2525-3 CrossRefGoogle Scholar
  12. Jung KA, Min HJ, Yoo SS, Kim HJ, Choi SN, Ha CY, Kim HJ, Kim TH, Jung WT, Lee OJ, Lee JS, Shim SG (2011) Drug-induced liver injury: twenty-five cases of acute hepatitis following ingestion of Polygonum multiflorum Thunb. Gut Liver 4:493–499CrossRefGoogle Scholar
  13. Karuppusamy S (2009) A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell culture. J Med Plant Res 3:1222–1239Google Scholar
  14. Kim YE (2017) Biosynthesis of isoflanovones and coumestrol from adventitious root and callus in Glycine max. Master thesis, Chungbuk National Universiry, Cheongju, Republic of Korea, pp 64–74Google Scholar
  15. Kim YS, Hahn EJ, Yeung EC, Paek KY (2003) Lateral root development and saponin accumulation as affected by IBA or NAA in adventitious root cultures of Panax ginseng C.A. Meyer. In Vitro Cell Dev Biol-Plant 39:245–249CrossRefGoogle Scholar
  16. Kim EM, Lee KJ, Chee KM (2004) Comparison in isoflavone contents between soybean and soybean sprouts of various soybean cultivar. Korean Nutr Soc 37:45–51Google Scholar
  17. Lee EJ, Park SY, Paek KY (2015) Enhancement strategies of bioactive compound production in adventitious root cultures of Eleutherococcus koreanum Nakai subjected to methyl jasmonate and salicylic acid elicitation through airlift bioreactors. Pant Cell Tissue Organ Cult 120:1–10CrossRefGoogle Scholar
  18. Liu HC, Wu W, Hou K, Chen JW, Zhao Z (2016) Deep sequencing reveals transcriptome re-programming of Polygonum multiflorum Thunb. roots to the elicitation with methyl jasmonate. Mol Genet Genomics 291:337–348CrossRefGoogle Scholar
  19. Matkowski A (2004) In vitro isoflavonoid production in callus from different organs of Pueraria lobate (Wild.) Ohw. J Plant Physiol 161:343–346CrossRefGoogle Scholar
  20. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479CrossRefGoogle Scholar
  21. Murthy HN, Dandin VS, Zhong JJ, Paek KY (2014) Strategies for enhanced production of plant secondary metabolites from cell and organ cultures. In: Paek KY, Murthy HN, Zhong JJ (eds) Production of biomass and bioactive compounds using bioreactor technology. Springer, Dordrecht, pp 471–508Google Scholar
  22. Murthy HN, Dandin VS, Paek KY (2016) Tools for biotechnological production of useful phytochemicals from adventitious root cultures. Phytochem Rev 15:129–145CrossRefGoogle Scholar
  23. Naik PM, Manohar SH, Praveen N, Upadhya V, Murthy HN (2012) Evaluation of bacoside A content in different accessions and various organ of Bacopa monnieri (L.) Wettst. J Herbs Spices Med Plants 18:387–395CrossRefGoogle Scholar
  24. Nhut DT, Hai NT, Huy NP, Chien HX, Nam NB (2013) New achievement in Panax vietnamensis research. In: Jain SM, Gupta SD (eds) Biotechnology of neglected and underutilized crops. Springer, Dordrecht, pp 43–57CrossRefGoogle Scholar
  25. Paek KY, Murthy HN, Hahn EJ, Zhong JJ (2009) Large scale culture of ginseng adventitious roots for production of ginsenosides. Adv Biochem Eng Biotechnol 113:151–176Google Scholar
  26. Park SY, Paek KY (2014) Bioreactor culture of shoots and somatic embryos of medicinal plants for production of bioactive compounds. In: Paek KY, Murthy HN, Zhong JJ (eds) Production of biomass and bioactive compounds using bioreactor technology. Springer, Dordrecht, pp 337–368Google Scholar
  27. Park HJ, Zhanga N, Park DK (2011) Topical application of Polygonum multiflorum extract induces hair growth of resting hair follicles through upregulating Shh and β-catenin expression in C57BL/6 mice. J Ethnopharmacol 135:369–375CrossRefGoogle Scholar
  28. Percival SS (2000) Use of echinacea in medicine. Biochem Pharmacol 60:155–158CrossRefGoogle Scholar
  29. Praveen N, Murthy HN (2010) Production of withanolide-A from adventitious root cultures of Withania somnifera. Acta Physiol Plant 32:1017–1022CrossRefGoogle Scholar
  30. Praveen N, Murthy HN (2014) Production of withanolides from cell and organ cultures of Withania somnifera (L.) Dunal. In: Paek KY, Murthy HN, Zhong JJ (eds) Production of biomass and bioactive compounds using bioreactor technology. Springer, Dordrecht, pp 285–316Google Scholar
  31. Tam HT, Nam NB, Chien HX, Cuong LK, Tai NT, Cuong NV, Huy NP, Huong TT, Hieu T, Linh NTN, Nhut DT (2015) Optimization of culture conditions and medium composition for adventitious root induction from leaves of Panax vietnamensis Ha et Grushv. Vietnam J Biotechnol 13:865–873Google Scholar
  32. Trinh TH, Tam HT, Ngan HTM, Tai NT, Huy NP, Chien HX, Nam NB, Luan VQ, Hien VT, Huong NTT, Ngoc PB, Ha CH, Nhut DT (2012) Influence of explant source, explant size and auxin on in vitro adventitious shoot regeneration ability of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.). Vietnam J Biotech 10:877–886Google Scholar
  33. Wu CH, Dewir YH, Hahn EJ, Paek KY (2006) Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol 49:193–199CrossRefGoogle Scholar
  34. Wu XQ, Chen XZ, Huang QC, Fang DM, Li GY, Zhang GL (2012) Toxicity of raw and processed roots of Polygonum multiflorum. Fitoterapia 3:469–475CrossRefGoogle Scholar
  35. Yu KW, Hahn EJ, Paek KY (2000) Production of adventitious ginseng roots using bioreactors. Korean J Plant Tissue Cult 27:309–315Google Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Horticultural Science, Division of Animal, Horticulture and Food SciencesChungbuk National UniversityCheongjuRepublic of Korea
  2. 2.WellGreen Co., Ltd., Chungbuk National UniversityCheongjuRepublic of Korea
  3. 3.Department of Forest Genetic ResourcesNational Institute of Forest ScienceSuwonRepublic of Korea

Personalised recommendations