Improvement of bioactive saponin accumulation in adventitious root cultures of Panax vietnamensis via culture periods and elicitation
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Saponin compounds released by Panax vietnamensis into the rhizosphere play critical roles in drug discovery. This study investigated the growth and saponin accumulation of P. vietnamensis adventitious root. Results showed the root growth and productivity of three-saponins (MR2, Rb1, and Rg1) were increased highest on the modified MS medium containing 7 mg/L IBA and 0.5 mg/L BA at 56 days. The root culture divided into three phases: (1) Growth phase 14–56 culture-days; (2) Stationary phase 56–70 days; and (3) Decline phase after 70 days. Saponin accumulation was significantly increased in the treatments of three plant-hormone-elicitors (JA, ABA, and SA), and two cell-wall elicitors (YE and CHN), but five elicitors inhibited formation lateral roots. Although contents of Rg1 (0.32%), Rb1 (0.85%), and MR2 (2.83%) were highest in JA treatment at 0.48 mM, 0.24 mM, and 0.96 mM JA respectively, maximum biomass as well as saponins productivity was observed in 150 mg/L YE. In addition, combination of YE and SA showed effective lower than the solitary YE treatment. The results suggest that adventitious root biomass of P. vietnamensis developed effectively in the modified MS medium after 56 days, and the culture could be scale-up in bioreactor 20 L.
KeywordsSaponin Adventitious root culture Elicitors Plant growth regulators P. vietnamensis
This study was supported by Tay Nguyen Institute for Scientific Research (VAST) and The Research Centre of Ginseng and Medicinal Materials in Vietnam.
NTNL and DTN conceived of the presented idea. NTNL, LKC, HTT, HTT, VQL, and VTH developed the theory and performed the computations. NTNL verified the analytical methods. DTN and NHL encouraged NTNL, LKC, HTT, HTT, VQL, and VTH to investigate the development of adventitious root and supervised the findings of this work. All authors discussed the results and contributed to the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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