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Sodium nitroprusside enhances callus induction and shoot regeneration in high value medicinal plant Canscora decussata

  • Sivakumar SubiramaniEmail author
  • Sathish Sundararajan
  • Hari Priya Sivakumar
  • Venkatesh Rajendran
  • Sathishkumar RamalingamEmail author
Original Article
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Abstract

The present investigation was carried out to demonstrate the plant morphogenetic potential of sodium nitroprusside (SNP) in a commercially important medical plant Canscora decussata. Leaf and nodal explants were used and highest callusing frequency (97.18%) was recorded for leaf explants supplemented with 1.0 mg/L NAA + 2.0 mg/L 2ip + 15 µM SNP. SNP enhanced multiple shoot formation in both nodal and leaf explants derived callus considerably in the media supplemented with 1.0 mg/L NAA + 15 µM SNP and 0.5 mg/L BAP + 15 µM SNP (98.32 and 96.12%) respectively. The root induction also drastically increased with the supplementation of SNP (10 µM) in combination with 1.0 mg/L IBA. Our results proved that SNP with other growth hormones act synergistically to enhance the in vitro responses including callus, shoot, and root induction. This optimized tissue culture system will be very useful for the mass propagation of C. decussata for both commercial and conservation aspects.

Keywords

Sodium nitroprusside Canscora decussata Plant growth regulators Mass multiplication 

Notes

Acknowledgements

Dr. S. Subiramani thanks National Post-Doctoral Fellowship (Sanction Order No: PDF/2016/002258/LS), Science and Engineering Research Board, Department of Science and Technology, Government of India. Mr. S. Sundararajan acknowledges ICMR (No.3/1/2/102/2018-Nut.) for fellowship support. We would also like to thank, University Grants Commission-Special Assistance Programme (UGC-SAP) and Department of Science and Technology-Fund for Improvement of S&T Infrastructure in Higher Educational Institutions (DST-FIST) for the financial support to carry out this research.

Author contributions

SS and SS designed, executed the experiments and prepared the manuscript. HPS and VR contributed substantially to the research in experimental and statistical analysis. SR mobilized the funds and critically evaluated the manuscript.

Compliance with ethical standards

Conflict of interest

All authors read, approved the manuscript and declare that there is no conflict of interest.

References

  1. Ambasta SP (1999) The useful plants of India. National Institute of Science Communication, New DelhiGoogle Scholar
  2. Beligni MV, Lamattina L (2000) Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants. Planta 210(2):215–221CrossRefGoogle Scholar
  3. Beligni MV, Lamattina L (2002) Nitric oxide interferes with plant photo-oxidative stress by detoxifying reactive oxygen species. Plant Cell Environ 25:737–748CrossRefGoogle Scholar
  4. Besson-Bard A, Pugin A, Wendehenne D (2008) New insights into nitric oxide signalling in plants. Annu Rev Plant Biol 59:21–39CrossRefGoogle Scholar
  5. Canter PH, Thomas H, Ernst E (2012) Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends Biotechnol 23:180–185CrossRefGoogle Scholar
  6. Carmona F, Pereira AMS (2013) Herbal medicines: old and new concepts, truths and misunderstandings. Rev Bras Farmacogn Braz J Pharmacogn 23:379–385CrossRefGoogle Scholar
  7. Chavan JJ, Nalawade AS, Gaikwad NB, Gurav RV, Dixit GB, Yadav SR (2014) An efficient in vitro regeneration of Ceropegia noorjahaniae: an endemic and critically endangered medicinal herb of the Western Ghats. Physiol Mol Biol Plants 20(3):405–410CrossRefGoogle Scholar
  8. Cornwell T, Pastor I, Fridlender B (2002) Plants and human health in the twenty-first century. Trends Biotechnol 20:522–531CrossRefGoogle Scholar
  9. Correa-Aragunde N, Graziano M, Lamattina L (2004) Nitric oxide plays a central role in determining lateral root development in tomato. Planta 218(6):900–905CrossRefGoogle Scholar
  10. Correa-Aragunde N, Graziano M, Chevalier C, Lamattina L (2006) Nitric oxide modulates the expression of cell cycle regulatory genes during lateral root formation in tomato. J Exp Bot 57:581–588CrossRefGoogle Scholar
  11. Cuiqin Y, Yaoguo Q, Xin S, Shu Y, Honghui L (2012) Propagation of Sedum spectabileBoreau in leaf culture in vitro. Notulae Bot Horti Agrobot Cluj-Napoca 40(1):107–112CrossRefGoogle Scholar
  12. Dikshit SK, Tewari PV, Dixit SP (1972) Anticonvulsant activity of C. decussata Roem and Schult. Indian J Physiol Pharmacol 16:81–83Google Scholar
  13. Dubey NK, Kumar R, Tripathi P (2004) Global promotion of herbal medicine: India’s opportunity. Curr Sci 86:37–41Google Scholar
  14. Ederli L, Reale L, Madeo L, Ferranti F, Gehring C, Fornaciari M, Romano B, Pasqualini S (2009) NO release by nitric oxide donors in vitro and in planta. Plant Physiol Biochem 47:42–48CrossRefGoogle Scholar
  15. El-Beltagi HS, Ahmed OK, Hegazy AE (2015) Molecular role of nitric oxide in secondary products production in Ginkgo biloba cell suspension culture. Notulae Bot Horti Agrobot Cluj-Napoca 43(1):12–18Google Scholar
  16. Floryszak-Wieczorek J, Milczarek G, Arasimowicz M, Ciszewski A (2006) Do nitric oxide donors mimic endogenous NO-related response in plants? Planta 224:1363–1372CrossRefGoogle Scholar
  17. Gaikwad NK, Moon UR, Bhadoria PS, Mitra A (2015) In vitro propagation of Canscora decussata Schult. and comparative assessment of anti-cholinesterase and antioxidant capacities of wild-harnessed and in vitro-grown plant extracts. Plant Cell Tiss Organ Cult 122(2):509–516CrossRefGoogle Scholar
  18. Gao Z, Lin Y, Wang X, Wang X, Wei M, Yang F, Shi Q (2012) Sodium nitroprusside (SNP) alleviates the oxidative stress induced by NaHCO3 and protects chloroplast from damage in cucumber. Afr J Biotechnol 11:6974–6982Google Scholar
  19. Gouvêa CMCP, Souza JF, Magalhães CAN, Martins IS (1997) NO releasing substances that induce growth elongation in maize root segments. Plant Growth Regul 21:183–187CrossRefGoogle Scholar
  20. Han X, Yang H, Duan K, Zhang X, Zhao H, You S, Jiang Q (2009) Sodium nitroprusside promotes multiplication and regeneration of Malus hupehensis in vitro plantlets. Plant Cell Tiss Organ Cult 96:29–34CrossRefGoogle Scholar
  21. Huang AX, She XP (2003) Effect of SNP on rooting of hypocotyls cutting from mung bean seedling. Acta Bot Boreal-Occident Sin 23:2196–2199Google Scholar
  22. Ivanova M, Van Staden J (2009) Nitrogen source, concentration, and NH4 +: NO3 ratio influence shoot regeneration and hyperhydricity in tissue cultured Aloe polyphylla. Plant Cell Tiss Organ Cult 99(2):167–174CrossRefGoogle Scholar
  23. Ivanova M, Van Staden J (2011) Influence of gelling agent and cytokinins on the control of hyperhydricity in Aloe polyphylla. Plant Cell Tiss Organ Cult 104(1):13–21CrossRefGoogle Scholar
  24. Jhanji S, Setia RC, Kaur N, Kaur P, Setia N (2012) Role of nitric oxide in cadmium-induced stress on growth, photosynthetic components and yield of Brassica napus L. J Environ Biol 33:1027–1032Google Scholar
  25. Jo MH, Ham IK, Lee AM, Lee ME, Song HN, Han HG, Woo SI (2002) Effects of sealing materials and photosynthetic photon flux of culture vessel on growth and vitrification in carnation plantlets in vitro. J Korean Soc Hort Sci 43(2):133–136Google Scholar
  26. Kadota M, Niimi Y (2003) Effects of cytokinin types and their concentrations on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots. Plant Cell Tiss Organ Cult 72:261–265CrossRefGoogle Scholar
  27. Kalra C, Babbar SB (2010) Nitric oxide promotes in vitro organogenesis in Linum usitatissimum L. Plant Cell Tiss Organ Cult 103:353–359CrossRefGoogle Scholar
  28. Kalra C, Babbar SB (2012) Stimulatory and period-specific effect of nitric oxide on in vitro caulogenesis in Albizzia lebbeck (L.) Benth. Acta Physiol Plant 34:387–392CrossRefGoogle Scholar
  29. Kaviani B (2014) Micropropagation of ten weeks (Matthiola incana) and lisianthus (Eustoma grandiflorum) (two ornamental plants) by using Kinetin (Kin), naphthalene acetic acid (NAA) and 2,4- dichlorophenoxyacetic acid (2,4-D). Acta Sci Pol Hortorum Cultus 13(1):141–154Google Scholar
  30. Khurana A, Khurana JP, Babbar SB (2011) Nitric oxide induces flowering in the duckweed Lemna aequinoctialis Welw. (Syn. L. paucicostata Hegelm.) under noninductive conditions. J Plant Growth Regul 30:378–385CrossRefGoogle Scholar
  31. Kohmura H, Araki H, Imoto M (1995) Micropropagation of ‘Yamatoimo’ Chinese yam (Dioscorea opposita) from immature leaves. Plant Cell Tiss Organ Cult 40:271–276CrossRefGoogle Scholar
  32. Kolberz Z, Bartha B, Erdei L (2008) Exogenous auxin-induced NO synthesis is nitrate reductase-associated in Arabidopsis thaliana root primordial. J Plant Physiol 165:967–975CrossRefGoogle Scholar
  33. Kousalya L, Bai VN (2016) Effect of growth regulators on rapid micropropagation and antioxidant activity of Canscora decussata (Roxb.) Roem. & Schult.—A threatened medicinal plant. Asian Pac J Reprod 5(2):161–170CrossRefGoogle Scholar
  34. Krasylenko YA, Yemets AI, Blume YB (2010) Functional role of nitric oxide in plants. Russ J Plant Physiol 57:451–461CrossRefGoogle Scholar
  35. Lamattina L, Garcia-Mata C, Graziano M, Pagnussat G (2003) Nitric oxide: the versatility of an extensive signal molecule. Annu Rev Plant Biol 54:109–136CrossRefGoogle Scholar
  36. Leshem YY (1996) Nitric oxide in biological systems. J Plant Growth Regul 18:155–169CrossRefGoogle Scholar
  37. Leshem YY, Haramaty E (1996) Plant aging, the emission of NO and ethylene and effect of NO-releasing compounds on growth of pea (Pisum sativum) foliage. J Plant Physiol 148:258–263CrossRefGoogle Scholar
  38. Leterrier M, Valderrama R, Chaki M, Airaki M, Palma JM, Barroso JB, Corpas FJ (2012) Function of nitric oxide under environmental stress conditions. In: Khan NA, Nazar R, Iqbal N, Anjum NA (eds) Phytohormones and abiotic stress tolerance in plants. Springer, Berlin, pp 99–113CrossRefGoogle Scholar
  39. Libourel IGL, Bethke PC, DeMichele R, Jones RL (2006) Nitric oxide gas stimulates germination of dormant Arabidopsis seeds: use of a flow through apparatus for delivery of nitric oxide. Planta 223:813–820CrossRefGoogle Scholar
  40. Madan B, Ghosh B (2002) Canscora decussata promotes adhesion of neutrophils to human umbilical vein endothelial cells. J Ethnopharmacol 79(2):229–235CrossRefGoogle Scholar
  41. Mahendran G, Narmatha Bai V (2014) Micropropagation, antioxidant properties and phytochemical assessment of Swertia corymbosa (Griseb.) Wight ex CB Clarke: a medicinal plant. Acta Physiol Plant 36(3):589–603CrossRefGoogle Scholar
  42. Malik SK, Chaudhary R, Kalia RK (2005) Rapid in vitro multiplication and conservation of Garcinia indica: a tropical medicinal three species. Sci Hort 106:539–553CrossRefGoogle Scholar
  43. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:173–197CrossRefGoogle Scholar
  44. Nagasawa A, Finer JJ (1989) Plant regeneration from embryogenic suspension culture of Chinese yam (Dioscorea opposita Thunb.). Plant Sci 60:263–271CrossRefGoogle Scholar
  45. Ötvos K, Pasternak TP, Miskolczi P, Domoki M, Dorjgotov D, Szucs A, Bottka S, Dudits D, Feher A (2005) Nitric oxide is required for, and promotes auxin-mediated activation of, cell division and embryogenic cell formation but does not influence cell cycle progression in alfalfa cell cultures. Plant J 43:849–860CrossRefGoogle Scholar
  46. Pagnussat GC, Simontacchi M, Puntarulo S, Lamattina L (2002) Nitric oxide is required for root organogenesis. Plant Physiol 129:954–956CrossRefGoogle Scholar
  47. Pagnussat GC, Lanteri ML, Lamattina L (2003) Nitric oxide and cyclic GMP are messengers in the indole acetic acid-induced adventitious rooting process. Plant Physiol 132:1241–1248CrossRefGoogle Scholar
  48. Park SW, Jeon JH, Kim HS, Park YM, Aswath C, Joung H (2004) Effect of sealed and vented gaseous microenvironments on the hyperhydricity of potato shoots in vitro. Sci Hortic 99(2):199–205CrossRefGoogle Scholar
  49. Raskin I, Ribnicky DM, Komarnytsky S, Ilic N, Poulev A, Borisjuk N (2002) Plants and human health in the twenty-first century. Trends Biotechnol 20:522–531CrossRefGoogle Scholar
  50. Samsampour D, Sadeghi F, Asadi M, Ebrahimzadeh A (2018) Effect of nitric oxide (NO) on the induction of callus and antioxidant capacity of Hyoscyamus niger under in vitro salt stress. J Appl Bot Food Qual 91:24–32Google Scholar
  51. Sarropoulou V, Maloupa E (2017) Effect of the NO donor “sodium nitroprusside”(SNP), the ethylene inhibitor “cobalt chloride” (CoCl 2) and the antioxidant vitamin E “α-tocopherol” on in vitro shoot proliferation of Sideritis raeseri Boiss. & Heldr. subsp. raeseri. Plant Cell Tiss Organ Cult 128(3):619–629CrossRefGoogle Scholar
  52. Sarropoulou V, Dimassi-Theriou K, Therios I (2015) Effect of sodium nitroprusside on micropropagation and biochemical parameters of CAB-6P and Gisela 6 cherry rootstocks. Turk J Biol 39(4):595–610CrossRefGoogle Scholar
  53. Schmidt BM, Ribnicky DM, Lipsky PE, Raskin I (2007) Revisiting the ancient concept of botanical therapeutics. Nat Chem Biol 3:360–366CrossRefGoogle Scholar
  54. Sethiya NK, Mishra SH (2014) Investigation of mangiferin, as a promising natural polyphenol xanthone on multiple targets of Alzheimer’s disease. J Biol Active Prod Nat 4(2):111–119Google Scholar
  55. Sethiya NK, Nahata A, Dixit VK (2008) Simultaneous spectrofluorimetric determination of scopoletin and mangiferin in a methanolic extract of Canscora decussata Schult. Asian J Trad Med 3(6):224–229Google Scholar
  56. Sethiya NK, Nahata A, Mishra SH, Dixit VK (2009) An update on Shankhpushpi, a cognition-boosting ayurvedic medicine. J Chin Integr Med 7(11):1001–1022CrossRefGoogle Scholar
  57. Sethiya NK, Nahata A, Dixit VK, Mishra SH (2012) Cognition boosting effect of Canscora decussata (a South Indian Shankhpushpi). Eur J Integr Med 4:113–121CrossRefGoogle Scholar
  58. Sethiya NK, Trivedi A, Mishra SH (2015) Rapid validated high performance thin layer chromatography method for simultaneous estimation of mangiferin and scopoletin in Canscora decussata (South Indian Shankhpushpi) extract. Rev Bras Farmacogn 25(3):193–198CrossRefGoogle Scholar
  59. Shekhawat MS, Kannan N, Manokari M (2015) In vitro propagation of traditional medicinal and dye yielding plant Morinda coreia Buch. Ham. S Afr J Bot 100:43–50CrossRefGoogle Scholar
  60. Shirdel M, Motallebi-Azar A, Masiha S, Mortazavi N, Matloobi M, Sharafi Y (2011) Effects of inorganic nitrogen source and NH4 +: NO3 ratio on proliferation of dog rose (Rosa canina). J Med Plants Res 5(18):4605–4609Google Scholar
  61. Sivanesan I, Song JY, Hwang SJ, Jeong BR (2011) Micropropagation of Cotoneaster wilsonii Nakai a rare endemic ornamental plant. Plant Cell Tiss Organ Cult 105(1):55–63CrossRefGoogle Scholar
  62. Sun BT, Jing Y, Chen KM, Song LL, Chen FJ, Zhang LX (2007) Protective effect of nitric oxide on iron deficiency-induced oxidative stress in maize (Zea mays). J Plant Physiol 164:536–543CrossRefGoogle Scholar
  63. Sung CH, Hong JK (2010) Sodium nitroprusside mediates seedling development and attenuation of oxidative stresses in Chinese cabbage. Plant Biotechnol Rep 4:243–251CrossRefGoogle Scholar
  64. Tewari RK, Kim S, Hahn EJ, Paek KY (2008) Involvement of nitric oxide induced NADPH oxidase in adventitious root growth and antioxidant defense in Panax ginseng. Plant Biotechnol Rep 2:113–122CrossRefGoogle Scholar
  65. Tun NN, Holk A, Scherer GFE (2001) Rapid increase of NO release in plant cell cultures induced by cytokinin. FEBS Lett 509:174–176CrossRefGoogle Scholar
  66. Upadhyay AS, Kumbhojkar MS (1993) Studies on ayurvedic drug shankhpushpi from western Maharashtra medicobotanical reported aspects. Bull Medicoethan Res 14:64–69Google Scholar
  67. Xu J, Yin H, Wang W, Mi Q, Liu X (2009) Effects of sodium nitroprusside on callus induction and shoot regeneration in micropropagated Dioscorea opposita. Plant Growth Regul 59(3):279CrossRefGoogle Scholar
  68. Yadav MK, Gaur AK, Garg GK (2003) Development of suitable protocol to overcome hyperhydricity in carnation during micropropagation. Plant Cell Tiss Organ Cult 72(2):153–156CrossRefGoogle Scholar
  69. Yu U, Zhao YQ, Zhao B, Ren S, Guo YD (2011) Influencing factors and structural characterization of hyperhydricity of in vitro regeneration in Brassica oleracea var. italica. Can. J Plant Sci 91:59–165Google Scholar
  70. Zamani M, Hakimi MH, Mosleh Arany A, Kiani B, Rashtian A (2014) The effects of salicylic acid (SA) and sodium nitroprusside (SNP) on physical and growth characteristics of Pinus eldarica. Bull Environ Pharmacol Life Sci 3:31–35Google Scholar
  71. Zandonadi DB, Santos MP, Dobbss LB, Olivares FL, Canellas LP, Binzel ML, Okorokova-Façanha AL, Façanha AR (2010) Nitric oxide mediates humic acids-induced root development and plasma membrane H+-ATPase activation. Planta 231:1025–1036CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Sivakumar Subiramani
    • 1
    Email author
  • Sathish Sundararajan
    • 1
  • Hari Priya Sivakumar
    • 1
  • Venkatesh Rajendran
    • 1
  • Sathishkumar Ramalingam
    • 1
    Email author
  1. 1.Plant Genetic Engineering Laboratory, Department of BiotechnologyBharathiar UniversityCoimbatoreIndia

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