Sodium nitroprusside enhances callus induction and shoot regeneration in high value medicinal plant Canscora decussata

  • Sivakumar SubiramaniEmail author
  • Sathish Sundararajan
  • Hari Priya Sivakumar
  • Venkatesh Rajendran
  • Sathishkumar RamalingamEmail author
Original Article


The present investigation was carried out to demonstrate the plant morphogenetic potential of sodium nitroprusside (SNP) in a commercially important medical plant Canscora decussata. Leaf and nodal explants were used and highest callusing frequency (97.18%) was recorded for leaf explants supplemented with 1.0 mg/L NAA + 2.0 mg/L 2ip + 15 µM SNP. SNP enhanced multiple shoot formation in both nodal and leaf explants derived callus considerably in the media supplemented with 1.0 mg/L NAA + 15 µM SNP and 0.5 mg/L BAP + 15 µM SNP (98.32 and 96.12%) respectively. The root induction also drastically increased with the supplementation of SNP (10 µM) in combination with 1.0 mg/L IBA. Our results proved that SNP with other growth hormones act synergistically to enhance the in vitro responses including callus, shoot, and root induction. This optimized tissue culture system will be very useful for the mass propagation of C. decussata for both commercial and conservation aspects.


Sodium nitroprusside Canscora decussata Plant growth regulators Mass multiplication 



Dr. S. Subiramani thanks National Post-Doctoral Fellowship (Sanction Order No: PDF/2016/002258/LS), Science and Engineering Research Board, Department of Science and Technology, Government of India. Mr. S. Sundararajan acknowledges ICMR (No.3/1/2/102/2018-Nut.) for fellowship support. We would also like to thank, University Grants Commission-Special Assistance Programme (UGC-SAP) and Department of Science and Technology-Fund for Improvement of S&T Infrastructure in Higher Educational Institutions (DST-FIST) for the financial support to carry out this research.

Author contributions

SS and SS designed, executed the experiments and prepared the manuscript. HPS and VR contributed substantially to the research in experimental and statistical analysis. SR mobilized the funds and critically evaluated the manuscript.

Compliance with ethical standards

Conflict of interest

All authors read, approved the manuscript and declare that there is no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Sivakumar Subiramani
    • 1
    Email author
  • Sathish Sundararajan
    • 1
  • Hari Priya Sivakumar
    • 1
  • Venkatesh Rajendran
    • 1
  • Sathishkumar Ramalingam
    • 1
    Email author
  1. 1.Plant Genetic Engineering Laboratory, Department of BiotechnologyBharathiar UniversityCoimbatoreIndia

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