Advertisement

Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 137, Issue 1, pp 181–185 | Cite as

Arabidopsis zinc finger homeodomain protein ZHD5 promotes shoot regeneration and confers other cytokinin-related phenotypes when overexpressed

  • Jin-Baek Kim
  • Jung-Youn Kang
  • Min Young Park
  • Mi-ran Song
  • Young Cheol Kim
  • Soo Young KimEmail author
Research Note
  • 108 Downloads

Abstract

We present the data showing that overexpression of the Arabidopsis zinc finger homeodomain protein ZHD5 promotes shoot regeneration and confers other cytokinin-associated phenotypes. To isolate protein factors that bind to the promoter element of ABF2, which is a key regulator of ABA responsive gene expression, we carried out yeast-one-hybrid screen. Four zinc finger homeodomain (ZF-HD) protein genes were identified from the screen. Expression study showed that one of the ZF-HD proteins, ZHD5, is induced by various cytokinins (CKs). We generated and analyzed ZHD5 overexpression (OX) lines to study its function. Our results revealed that ZHD5 promoted shoot regeneration efficiency significantly, obviating CK requirement. Other CK-related phenotypes, such as enhanced cell proliferation and pathogen resistance, were also observed. At the molecular level, changes in the expression levels of a number of genes relevant to the phenotypes were observed. Collectively, our results provde strong evidence that ZHD5 is involved in shoot regeneration and other aspects of CK response.

Keywords

Zinc finger homeodomain (ZF-HD) Shoot regeration Cytokinin One-hybrid screen 

Abbreviations

ZF-HD

Zinc finger homeodoamin

CK

Cytokinin

RT-PCR

Coupled reverse transcription—polymerase chain reaction

OX

Overexpression

Notes

Acknowledgements

This work was supported in part by the Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Republic of Korea (Nos. 2014R1A1A2056798 and 2016R1D1A1B03933911 to S.Y.K.). The authors are grateful to the Kumho Life Science Laboratory of Chonnam National University for providing equipment and plant growth facilities. We thank Min-young Im for her technical assistance during initial stage of this work.

Author contributions

JBK, JYK, MYP, and MS designed and performed experiments. YCK designed and performed pathogen infection experiments. SYK designed experiments and wrote paper.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11240_2018_1546_MOESM1_ESM.docx (21 kb)
Supplementary Data 1. Materials and Methods. (DOCX 21 KB)
11240_2018_1546_MOESM2_ESM.pptx (11.1 mb)
Supplementary Data 2. Supplementary Figures. (PPTX 11322 KB)
11240_2018_1546_MOESM3_ESM.docx (16 kb)
Supplementary Data 3. Supplementary Table S1. (DOCX 15 KB)

References

  1. Choi H, Hong J, Ha J, Kang J, Kim SY (2000) ABFs, a family of ABA-responsive element binding factors. J Biol Chem 275:1723–1730CrossRefGoogle Scholar
  2. Figueiredo DD, Barros PM, Cordeiro AM, Serra TS, Lourenco T, Chander S, Oliveira MM, Saibo NJM (2012) Seven zinc-finger transcription factors are novel regulators of the stress responsive gene OsDREB1B. J Exp Bot 63:3643–3656.  https://doi.org/10.1093/jxb/ers035 CrossRefGoogle Scholar
  3. Fujita Y, Fujita M, Shinozaki K, Yamaguchi-Shinozaki K (2011) ABA-mediated transcriptional regulation in response to osmotic stress in plants. J Plant Res 124:509–525.  https://doi.org/10.1007/s10265-011-0412-3 CrossRefGoogle Scholar
  4. Hong SY, Kim OK, Kim SG, Yang MS, Park CM (2011) Nuclear import and DNA binding of the ZHD5 transcription factor is modulated by a competitive peptide inhibitor in Arabidopsis. J Biol Chem 286:1659–1668.  https://doi.org/10.1074/jbc.M110.167692 CrossRefGoogle Scholar
  5. Hu W, dePamphilis CW, Ma H (2008) Phylogenetic analysis of the plant-specific zinc finger-homeobox and mini zinc finger gene families. J Integr Plant Biol 50:1031–1045.  https://doi.org/10.1111/j.1744-7909.2008.00681.x CrossRefGoogle Scholar
  6. Kang J, Choi H, Im M, Kim SY (2002) Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell 14:343–357CrossRefGoogle Scholar
  7. Kieber JJ, Schaller GE (2014) Cytokinins. Arabidopsis Book 12:e0168.  https://doi.org/10.1199/tab.0168 CrossRefGoogle Scholar
  8. Naseem M, Kaltdorf M, Dandekar T (2015) The nexus between growth and defence signalling: auxin and cytokinin modulate plant immune response pathways. J Exp Bot 66:4885–4896.  https://doi.org/10.1093/jxb/erv297 CrossRefGoogle Scholar
  9. Tan QKG, Irish VF (2006) The Arabidopsis zinc finger-homeodomain genes encode proteins with unique biochemical properties that are coordinately expressed during floral development. Plant Physiol 140:1095–1108.  https://doi.org/10.1104/pp.105.070565 CrossRefGoogle Scholar
  10. Tran LS, Nakashima K, Sakuma Y, Osakabe Y, Qin F, Simpson SD, Maruyama K, Fujita Y, Shinozaki K, Yamaguchi-Shinozaki K (2007) Co-expression of the stress-inducible zinc finger homeodomain ZFHD1 and NAC transcription factors enhances expression of the ERD1 gene in Arabidopsis. Plant J 49:46–63.  https://doi.org/10.1111/j.1365-313X.2006.02932.x CrossRefGoogle Scholar
  11. Wang L, Hua D, He J, Duan Y, Chen Z, Hong X, Gong Z (2011) Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in Arabidopsis. Plos Genet 7:e1002172.  https://doi.org/10.1371/journal.pgen.1002172 CrossRefGoogle Scholar
  12. Windhovel A, Hein I, Dabrowa R, Stockhaus J (2001) Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia. Plant Mol Biol 45:201–214CrossRefGoogle Scholar
  13. Yoshida T, Fujita Y, Sayama H, Kidokoro S, Maruyama K, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K (2010) AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation. Plant J 61:672–685.  https://doi.org/10.1111/j.1365-313X.2009.04092.x CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life SciencesChonnam National UniversityGwangjuSouth Korea
  2. 2.Department of Applied Biology, College of Agriculture and Life SciencesChonnam National UniversityGwangjuSouth Korea

Personalised recommendations