Arabidopsis zinc finger homeodomain protein ZHD5 promotes shoot regeneration and confers other cytokinin-related phenotypes when overexpressed
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We present the data showing that overexpression of the Arabidopsis zinc finger homeodomain protein ZHD5 promotes shoot regeneration and confers other cytokinin-associated phenotypes. To isolate protein factors that bind to the promoter element of ABF2, which is a key regulator of ABA responsive gene expression, we carried out yeast-one-hybrid screen. Four zinc finger homeodomain (ZF-HD) protein genes were identified from the screen. Expression study showed that one of the ZF-HD proteins, ZHD5, is induced by various cytokinins (CKs). We generated and analyzed ZHD5 overexpression (OX) lines to study its function. Our results revealed that ZHD5 promoted shoot regeneration efficiency significantly, obviating CK requirement. Other CK-related phenotypes, such as enhanced cell proliferation and pathogen resistance, were also observed. At the molecular level, changes in the expression levels of a number of genes relevant to the phenotypes were observed. Collectively, our results provde strong evidence that ZHD5 is involved in shoot regeneration and other aspects of CK response.
KeywordsZinc finger homeodomain (ZF-HD) Shoot regeration Cytokinin One-hybrid screen
Zinc finger homeodoamin
Coupled reverse transcription—polymerase chain reaction
This work was supported in part by the Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Republic of Korea (Nos. 2014R1A1A2056798 and 2016R1D1A1B03933911 to S.Y.K.). The authors are grateful to the Kumho Life Science Laboratory of Chonnam National University for providing equipment and plant growth facilities. We thank Min-young Im for her technical assistance during initial stage of this work.
JBK, JYK, MYP, and MS designed and performed experiments. YCK designed and performed pathogen infection experiments. SYK designed experiments and wrote paper.
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Conflict of interest
The authors declare that they have no conflict of interest.
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