Advertisement

Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 137, Issue 1, pp 173–179 | Cite as

A high production of flavonoids and anthraquinones via adventitious root culture of Oplopanax elatus and evaluating antioxidant activity

  • L. Han
  • X. C. Piao
  • J. Jiang
  • X. L. Jiang
  • C. R. Yin
  • M. L. LianEmail author
Research Note
  • 95 Downloads

Abstract

Adventitious root (AR) culturing is an effective approach for obtaining bioactive compounds from the endangered plant species of Oplopanax elatus Nakai. In the present study, flavonoids and anthraquinones, both important bioactive compounds were identified via HPLC-ESI–MS/MS, and flavonoid monomers of quercetin and kaempferide and anthraquinone monomers of aloe-emodin, rhein, and emodin were detected in O. elatus AR cultures. To enhance synthesis of both compounds, methyl jasmonate (MeJA) was used as the elicitor to treat ARs. At 200 µM, MeJA significantly increased the contents of quercetin, aloe-emodin, rhein, and emodin, whereas 225 µM was the optimal concentration for kaempferide accumulation. The antioxidant property in ARs was evaluated by determining activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and alkyl radical scavenging using the an electron spin resonance spectrometer. The IC50 of DPPH (6.2 µg/mL) and alkyl (20.2 µg/mL) radical scavenging activities with MeJA treatment were lower than that of the control, thereby indicating that ARs treated with MeJA possess stronger antioxidant activity. Hence, AR cultures can be deployed as raw materials or additives in the production of antioxidant-related O. elatus products using the MeJA as elicitor.

Keywords

Adventitious root Flavonoids Anthraquinones Elicitation Methyl jasmonate Antioxidant 

Notes

Acknowledgements

This study was sponsored by the Jilin Scientific and Technological Development Program (20180101278JC) and the National Natural Science Foundation of China (31660080 and 21662038).

Author contributions

LH and XLJ performed AR culture experiments. XCP and JJ conducted the experiment of antioxidant experiment. CRY analyzed bioactive compounds. MLL designed experiments and wrote the manuscript.

Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflict of interest.

Supplementary material

11240_2018_1543_MOESM1_ESM.docx (154 kb)
Supplementary material 1 (DOCX 154 KB)

References

  1. Ahn JK, Lee WY, Park EJ (2010) Effect of methyl jasmonate on the root growth and the eleutheroside accumulation in the adventitious root culture of Eleutherococcus senticosus. J Kor For Soc 99:331–336Google Scholar
  2. Carmona-Jiménez Y, García-Moreno MV, Igartuburu JM, Barroso CG (2014) Simplification of the DPPH assay for estimating the antioxidant activity of wine and wine by-products. Food Chem 165:198–204CrossRefGoogle Scholar
  3. Cui HY, Baque MA, Lee EJ, Paek KY (2013) Scale-up of adventitious root cultures of Echinacea angustifolia in a pilot-scale bioreactor for the production of biomass and caffeic acid derivatives. Plant Biotechnol Rep 7:297–308CrossRefGoogle Scholar
  4. Cui XH, Murthy HN, Paek KY (2014) Pilot-scale culture of Hypericum perforatum L. adventitious roots in airlift bioreactors for the production of bioactive compounds. Appl Biochem Biotechnol 174:784–792CrossRefGoogle Scholar
  5. Faizah H, Tanjung M, Purnobasuk H, Wulan YS (2018) Biomass and flavonoid production of Gynura procumbens (L.) Merr adventitious root culture in baloon-type bubble-bioreactor influenced by elicitation. Asian J Plant Sci 17:107–119CrossRefGoogle Scholar
  6. Hahn EJ, Kim YS, Yu KW, Jeong CS, Paek KY (2003) Adventitious root cultures of Panax ginseng C.A. Meyer and ginsenoside production through large-scale bioreactor system. J Plant Biotechnol 5:1–6Google Scholar
  7. Heim KE, Tagliaferro AR, Bobilya DJ (2002) Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J Nutr Biochem 13:572–584CrossRefGoogle Scholar
  8. Hwang HJ, Song G, Kim MH, Do SG, Bae KH (2013) Increasement of antioxidative activity in Codonopsis lanceolata adventitious root treated by methyl jasmonate and salicylic acid. J Plant Biotechnol 40:178–183CrossRefGoogle Scholar
  9. Jiang YJ, Piao XC, Liu JS, Jiang J, Lian ZX, Kim MJ, Lian ML (2015) Bioactive compound production by adventitious root culture of Oplopanax elatus in balloon-type airlift bioreactor systems and bioactivity property. Plant Cell Tissue Organ Cult 123:413–425CrossRefGoogle Scholar
  10. Jiang XL, Piao XC, Gao R, Jin MY, Jiang J, Jin XH, Lian ML (2017) Improvement of bioactive compound accumulation in adventitious root cultures of an endangered plant species, Oplopanax elatus. Acta Physiol Plant 39:226CrossRefGoogle Scholar
  11. Lee EJ, Park SY, Paek KY (2015) Enhancement strategies of bioactive compound production in adventitious root cultures of Eleutherococcus koreanum Nakai subjected to methyl jasmonate and salicylic acid elicitation through airlift bioreactors. Plant Cell Tissue Organ Cult 120:1–10CrossRefGoogle Scholar
  12. Lin L, Du H (2018) An anthraquinone compound and its protective effects against homocysteine-induced cytotoxicity and oxidative stress. Spectrochim Acta A 202:314–318CrossRefGoogle Scholar
  13. Peng XY, Xiong YL, Kong BH (2009) Antioxidant activity of peptide fractions from whey protein hydrolysates as measured by electron spin resonance. Food Chem 113:196–201CrossRefGoogle Scholar
  14. Perassolo M, Cardillo AB, Mugas ML, Montoya SCN, Giulietti AM, Talou JR (2017) Enhancement of anthraquinone production and release by combination of culture medium selection and methyl jasmonate elicitation in hairy root cultures of Rubia tinctorum. Ind Crop Prod 105:124–132CrossRefGoogle Scholar
  15. Shikov AN, Pozharitskaya ON, Makarov VG, Yang WZ, Guo DA (2014) Oplopanax elatus (Nakai) Nakai: chemistry, traditional use and pharmacology. Chin J Nat Med 12:721–729Google Scholar
  16. Sun W, He YS, Xu LH, Zhang BY, Qi LW, Yang J, Li P, Wen XD (2016) Pharmacokinetic profiles of falcarindiol and oplopandiol in rats after oral administration of polyynes extract of Oplopanax elatus. Chin J Nat Med 14:714–720Google Scholar
  17. Wang JW, Wu JY (2005) Nitric oxide is involved in methyl jasmonate-induced defense responses and secondary metabolism activities of Taxus cells. Plant Cell Physiol 46:923–930CrossRefGoogle Scholar
  18. Wang J, Li JL, Li J, Li JX, Liu SJ, Huang LQ, Gao WY (2017) Production of active compounds in medicinal plants: from plant tissue culture to biosynthesis. Chin Herb Med 9:115–125CrossRefGoogle Scholar
  19. Wei YH, Wu XA, Chen L, Zhang CZ (2005) Study on determination methods of anthraquinones component of rhubarb. J Lanzhou Univ 31:13–14Google Scholar
  20. Xu QL, Mao XJ, Song XN, Ma Y, Xiong HL, Tong H (2010) HPLC simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in Liuwei Anxiao capsules. Chin J Pharm Anal 30:1840–1844Google Scholar
  21. Yin SS, Gao WY, Liang YY, Wang J, Liu H, Wei CL, Zuo BM (2013) Influence of sucrose concentration and phosphate source on biomass and metabolite accumulation in adventitious roots of Pseudostellaria heterophylla. Acta Physiol Plant 35:1579–1585CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Key Laboratory of Natural Resources of Changbai Mountain and Functional Moleculars (Yanbian University)Ministry of EducationYanjiChina

Personalised recommendations