The association of fraser photinia and its beneficial bacterium (PGB_invit) provided in vitro storage without subculture
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Endophytes play crucial roles due to their beneficial influence on plant development, growth, fitness, and diversification. Due to these important capabilities, they have received attention from the scientific community and many papers have been published recently about their beneficial role in in vivo and in vitro plant propagation. However, up to now, there is no research on utilization of these microbial endophytes in prolongation of in vitro storage. Thus, the aim of this study is to assess the influence of fraser photinia associated and putatively endophytic bacterium (Plant Growth Bacteria_ in vitro; PGB_invit) on in vitro storage of its host. When pure strain of the bacterium was inoculated, it enabled the storage of microshoots up to 16 months at 25 °C without requiring periodic subculture while control (unincubated with PGB_invit.) microshoots died after 2 months of storage without subculture as in vitro plant cultures definitely need periodic subcultures (once in every 4–6 weeks) in order to renew media and gaseous atmosphere. Moreover, while the presence of virulence (vir D1), auxin (aux1), and cytokinin (ipt) production genes was confirmed in plasmid DNA of the bacterium, nitrogen fixing gene (nifH) was detected by the PCR analysis using bacterial culture. Overall results demonstrated that with these capabilities PGB_invit could be useful for in vitro conservation of fraser photinia.
The novelty is the supplementation of in vitro plant growth without either periodic renewal of the media or decreasing the culture temperature by means of a beneficial plant-bacterium interaction.
KeywordsAux1 Endophytic Ipt NifH Plant growth promoting bacterium
YÖÇ and ÖA designed the research project; HA and EK carried out the plant storage analyses; IS and ÖA carried out the molecular analysis; IS, ÖA and YÖÇ wrote the paper.
This research was funded by a grant from TUBITAK (#KBAG 114Z579). A partial support was also obtained from Gebze Technical University (2014-A-09).
Compliance with ethical standards
Conflict of interest
There is no conflict of interest.
- Akkaya Ö, Gül Şeker M, Özden Çiftçi Y (2019) Plant growth promoting microbiome network. In: Dr SJ (ed) Microbes and the environment: plant-soil-microbe-environment interactions on agricultural production, pollution management and waste recycling towards sustainable development (in press)Google Scholar
- Cabanas CGL, Schiliro E, Valverde-Corredor A, Mercado-Blanco J (2014) The biocontrol endophytic bacterium Pseudomonas fluorescens PICF7 induces systemic defense responses in aerial tissues upon colonization of olive roots. Front Microbiol 5:1–14. https://doi.org/10.3389/fmicb.2014.00427 Google Scholar
- Camilleri C, Jouanin L (1991) The TR_DNA region carrying the auxin synthesis genes of the Agrobacterium rhizogenes agronine-type plasmid pRiA4: nucleotide sequence analysis and introduction into tobacco plants. Mol Plant Microbe Interact 4:155–162. https://doi.org/10.1094/MPMI-4-155 CrossRefGoogle Scholar
- Dias ACF, Costa FEC, Andreote FD, Lacava PT, Teixeira MA, Assumpçao LC, Araujo WL, Azevedo JL, Melo IS (2009) Isolation of micropropagated strawberry endophytic bacteria and assessment of their potential for plant growth promotion. World J Microbiol Biotechnol 25:189–195. https://doi.org/10.1007/s11274-008-9878-0 CrossRefGoogle Scholar
- Haas JH, Moore LW, Ream W, Manulis S (1995) Universal PCR primers for detection of phytopathogenic Agrobacterium strains. Appl Environ Microbiol 61:2879–2884Google Scholar
- Hardoim PR, van Overbeek LS, Berg G, Pırttila AM, Compant S, Campisano A, Döring M, Sessitsch A (2015) The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes. Microbiol Mol Biol Rev 79:293–320. https://doi.org/10.1128/MMBR.00050-14 CrossRefGoogle Scholar
- Herman EB (2004) Recent advances in plant tissue culture viii. Microbial contaminants in plant tissue cultures: solutions and opportunities 1996–2003. Agritech Consultants, Inc, New YorkGoogle Scholar
- Jasim B, Geethu PR, Mathew J, Radhakrishnan EK (2015) Effect of endophytic Bacillus sp. from selected medicinal plants on growth promotion and diosgenin production in Trigonella foenum graecum. Plant Cell Tissue Organ Cult 122:565–572. https://doi.org/10.1007/s11240-015-0788-1 CrossRefGoogle Scholar
- Jimtha JC, Smitha PV, Anisha C, Deepthi T, Meekha G, Radhakrishnan EK, Gayatri GP, Remakanthan A (2014) Isolation of endophytic bacteria from embryogenic suspension culture of banana and assessment of their plant growth promoting properties. Plant Cell Tissue Organ Cult 118:57–66. https://doi.org/10.1007/s11240-014-0461-0 CrossRefGoogle Scholar
- Koutsompogeras P, Kyriacou A, Zabetakis I (2007) The formation of 2,5-dimethyl-4-hydroxy-2H-furan-3-one by cell free extracts of Methylobacterium extorquens and strawberry (Fraga-ria ananassa cv. Elsanta). Food Chem 104:1654–1661. https://doi.org/10.1016/j.foodchem.2007.03.025 CrossRefGoogle Scholar
- Kulkarni VM, Ganapathi TR, Bapat VA, Rao PS (2004) Establishment of cell-suspension cultures in banana cv. Gr and Naine and evaluation of its sensitivity to gamma-irradiation. Curr Sci 86:902–904Google Scholar
- Lemoigne M (1926) Produit de d´eshydratation et de polym´erisation de l’acide b-oxybutyrique. Bull Soc Chem Biol 8:770–782Google Scholar
- Marascuilo LA, McSweeney M (1977) Nonparametric and distribution-free method for the social sciences. CA Brooks/Cole Publishing Company, MontereyGoogle Scholar
- Muromtsev GS, Chkanikov DI, Kulaeva ON, Gamburg KZ (1987) Osnovy khimicheskoi regulyatsii rosta I productivnosti rastenii (Basics of chemical regulation of plant growth and productivity). Agropromizdat, MoscowGoogle Scholar
- Patle PN, Navnage NP, Ramteke PR (2018) Endophytes in plant system: roles in growth promotion, mechanism and their potentiality in achieving agriculture sustainability. Int J Chem Stud 6:270–274Google Scholar
- Perez-Rosales E, Alcaraz-Meléndez L, Puente ME, Vázquez-Juárez R, Zenteno-Savín T, Morales-Bojórquez E (2018) Endophytic bacteria isolated from wild jojoba (Simmondsia chinensis L. [Schneider]) roots improve in vitro propagation. Plant Cell Tissue Organ Cult 135:515–522. https://doi.org/10.1007/s11240-018-1483-9 CrossRefGoogle Scholar
- Pham NT, Meier-Dinkel A, Höltken AM, Quambusch M, Mahnkopp F, Winkelmann T (2017) Endophytic bacterial communities in in vitro shoot cultures derived from embryonic tissue of hybrid walnut (Juglans x intermedia). Plant Cell Tissue Organ Cult 130:153–165. https://doi.org/10.1007/s11240-017-1211-x CrossRefGoogle Scholar
- Quambusch M, Brümmer J, Haller K, Winkelmann T, Bartsch M (2016) Dynamics of endophytic bacteria in plant in vitro culture—quantification of three bacterial strains in Prunus avium in different plant organs and in vitro culture phases. Plant Cell Tissue Organ Cult 126:305–317. https://doi.org/10.1007/s11240-016-0999-0 CrossRefGoogle Scholar
- Rogowsky PM, Powell BS, Shirasu K, Lin TS, Morel P, Zyprian EM, Steck TR, Kado CI (1990) Molecular characterization of the vir regulon of Agrobacterium tumefaciens: complete nucleotide sequence and gene organization of the 28.63-kbp regulon cloned as a single unit. Plasmid 23:85–106. https://doi.org/10.1016/0147-619x(90)90028-B CrossRefGoogle Scholar
- Thomas P (2004a) In vitro decline in plant cultures: detection of a legion of covert bacteria as the cause for degeneration of long-term micropropagated triploid watermelon cultures. Plant Cell Tissue Org Cult 77:173–179. https://doi.org/10.1023/B:TICU.0000016824.09108.c8 CrossRefGoogle Scholar
- Thomas P (2004b) A three-step screening procedure for detection of covert and endophytic bacteria in plant tissue cultures. Curr Sci 87:67–72Google Scholar
- Thomas P (2007) Isolation and identification of five alcohol defying Bacillus spp. covertly associated with in vitro culture of seedless watermelon. Curr Sci 92:983–987Google Scholar
- Thomas P, Prabhakara BS, Pitchaimuthu M (2006) Cleansing the long-term micropropagated triploid watermelon cultures from covert bacteria and field testing the plants for clonal fidelity and fertility during the 7–10 year period in vitro. Plant Cell Tissue Org Cult 85:317–329. https://doi.org/10.1007/s11240-006-9083-5 CrossRefGoogle Scholar
- Vigani G, Rolli E, Marasco R, Dell’Orto M, Michoud G, Soussi A, Raddadi N, Borin S, Sorlini C, Zocchi G, Daffonchio D (2018) Root bacterial endophytes confer drought resistance and enhance expression and activity of a vacuolar H+- pumping pyrophosphatease in pepper plants. Environ Microbiol. https://doi.org/10.1111/1462-2920.14272 Google Scholar