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Evaluation of secondary metabolites and antioxidant activity in Dracocephalum polychaetum Bornm. cell suspension culture under magnetite nanoparticles and static magnetic field elicitation

  • Marzieh Taghizadeh
  • Fatemeh Nasibi
  • Khosrow Manouchehri Kalantari
  • Faezeh Ghanati
Original Article
  • 31 Downloads

Abstract

Suspension-cultured Dracocephlum polychaetum Bornm. is a wild medicinal herb native to Iran, treated with a static magnetic field (SMF) and Fe2O3 magnetic nanoparticles (MNP). The effect of SMF (30 mT), Fe2O3 MNP (100 ppm) and the combination of these treatments on phenolic metabolism and the medicinal compounds were examined by high performance liquid chromatography and UV–Vis spectrophotometer. The activity of polyphenol oxidase and phenylalanine ammonialyase, as well as the content of total phenolics, flavonoid, anthocyanins, lignin and malondialdehyde in all treated cells, showed a significant difference with control. The intracellular content of rosmarinic acid, naringin, apigenin, thymol, carvacrol, quercetin and rutin, in all treated cells increased considerably. Their quantities also increased significantly in the treated cell culture media. These results suggest this increase is due to the increased production and secretion of the medicinal compounds from cells to the culture media upon application of SMF and Fe2O3 MNP.

Keywords

Suspension cultured cells Magnetite nanoparticles Medicinal compounds Static magnetic field Antioxidant activity Secondary metabolite 

Abbreviations

MNP

Fe2O3 magnetic nanoparticles

SMF

Static magnetic field

PPO

Polyphenol oxidase

PAL

Phenylalanine ammonia lyase

MDA

Malondialdehyde

Notes

Acknowledgements

This study was supported by Shahid Bahonar University of Kerman and Tarbiat Modares University of Tehran, Iran.

Author contributions

MT and FN devised the project, the main conceptual ideas and proof outline. MT and FGh developed the theory. MT carried out the experiment and collected data. MT verified the analytical methods, and. analyzed and interpreted the data. MT wrote the manuscript. FN and KhMK. contributed to the final version of the manuscript. All authors supervised the project and discussed the results and contributed to the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Marzieh Taghizadeh
    • 1
  • Fatemeh Nasibi
    • 1
    • 2
  • Khosrow Manouchehri Kalantari
    • 1
  • Faezeh Ghanati
    • 3
  1. 1.Department of Plant BiologyShahid Bahonar University of KermanKermanIran
  2. 2.Research and Technology Institute of Plant Production (RTIPP)Shahid Bahonar University of KermanKermanIran
  3. 3.Department of Plant Biology, Faculty of Biological ScienceTarbiat Modares UniversityTehranIran

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