In vitro photoautotrophic acclimatization, direct transplantation and ex vitro adaptation of rubber tree (Hevea brasiliensis)

  • Rujira Tisarum
  • Thapanee Samphumphung
  • Cattarin Theerawitaya
  • Wittaya Prommee
  • Suriyan Cha-um
Original Article
  • 144 Downloads

Abstract

We investigated the effect of carbon dioxide (CO2)-ambient (350 µmol CO2 mol−1) and CO2-enriched (1500 µmol CO2 mol−1) conditions of in vitro photoautotrophic system on two cultivars, ‘RRIM600’ and ‘RRIT413’ of rubber tree (Hevea brasiliensis) in an acclimatization process of 45 days. Survival percentage of in vitro rubber tree plantlets derived from somatic embryos under ambient CO2 was better than those under CO2-enriched conditions, especially in cv. ‘RRIT413’. Subsequently, the survival rate of ex vitro transplanted plantlets was similar to the in vitro plantlets and abnormal morphological characters such as light-green leaves (SPAD), small leaves in cv. ‘RRIT413’ acclimatized under CO2-enriched conditions were demonstrated 30 days after the plantlets were transferred into the soil. Maximum quantum yield of PSII, photon yield of PSII, stomatal conductance and transpiration rate in cv. ‘RRIT413’ acclimatized under CO2-enriched conditions were sharply declined by 39.0, 50.6, 47.1 and 45.8%, respectively as compared to those acclimatized under ambient CO2 conditions. In contrast, the in vitro acclimatized plantlets of cv. ‘RRIM600’ were un-responsive under both ambient- and enriched-CO2 conditions. In conclusion, genotypic dependent in response to CO2 enriched conditions in in-vitro acclimatization of rubber tree plantlets was evidently demonstrated as a key result to regulate plant growth and development in ex vitro environments. Interestingly, soluble sugar contents (sucrose, glucose and fructose) were increased after transplanting the plantlets of cv. ‘RRIM600’ acclimatized under CO2-enriched condition into the soil and thus, can be considered as an adaptive indicator of ex vitro adaptation.

Keywords

Chlorophyll fluorescence CO2 enrichment Net photosynthetic rate SPAD Survival percentage 

Notes

Acknowledgements

The authors wish to thank Rubber Research Institute of Thailand, Department of Agricultural, Ministry of Agricultural and Cooperative, as funding source and partially support by National Science and Technology Development Agency.

Author Contributions

The experiment design, statistical analysis and manuscript preparation were prepared by SC, somatic embryogenesis of rubber tree was provided by WP, in vitro acclimatization and transplantation was processed by TS, soluble sugar assay was done by CT and physiological and morphological data was recorded by RT. All authors were involved with editors of all versions, and agreed to the final version for publication, assuming public responsibility for the results.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Beruto M, Debergh P (2004) Micropropagation of Ranunculus asiaticus: a review and perspectives. Plant Cell Tiss Org Cult 77:221‒230CrossRefGoogle Scholar
  2. Carron MP, Enjalric F, Lardet L, Deschamps A (1989) Rubber (Hevea brasiliensis Müll. Arg.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 5, Trees II. Springer, Berlin, pp 222–245Google Scholar
  3. Carvalho L, Amâncio S (2002a) Effect of ex vitro conditions on growth and acquisition of autotrophic behaviour during the acclimatisation of chestnut regenerated in vitro. Sci Hortic 95:151‒164CrossRefGoogle Scholar
  4. Carvalho L, Amâncio S (2002b) Antioxidant defense system in plantlets transferred from in vitro to ex vitro: effects of increasing light intensity and CO2 concentration. Plant Sci 162:33‒40CrossRefGoogle Scholar
  5. Carvalho L, Osório ML, Chaves MM, Amâncio S (2001) Chlorophyll fluorescence as an indicator of photosynthetic functioning of in vitro grapevine and chestnut plantlets under ex vitro acclimatization. Plant Cell Tiss Org Cult 67:271‒280CrossRefGoogle Scholar
  6. Carvalho L, Santos P, Amâncio S (2002) Effect of light intensity and CO2 concentration on growth and the acquisition of in vivo characteristics during acclimatization of grapevine regenerated in vitro. Vitis 41:1–6Google Scholar
  7. Chandra S, Bandopadhyay R, Kumar V, Chandra R (2010) Acclimatization of tissue cultured plantlets: from laboratory to land. Biotechnol Lett 32:1199‒1205CrossRefGoogle Scholar
  8. Cha-um S, Supaibulwatana K, Kirdmanee C (2007) Glycinebetaine accumulation, physiological characterizations and growth efficiency in salt-tolerant and salt-sensitive lines of indica rice (Oryza sativa L. ssp. indica) in response to salt stress. J Agron Crop Sci 193:157‒166CrossRefGoogle Scholar
  9. Cha-um S, Chanseetis C, Chintakovid W, Pichakum A, Supaibulwatana K (2011) Promoting root induction and growth of in vitro macadamia (Macadamia tetraphylla L. ‘Keaau’) plantlets using CO2-enriched photoautotrophic conditions. Plant Cell Tiss Org Cult 106:435‒444CrossRefGoogle Scholar
  10. Gupta AK, Kaur N (2005) Sugar signalling and gene expression in relation to carbohydrate metabolism under abiotic stress in plants. J Biosci 30:761‒776CrossRefGoogle Scholar
  11. Haque SM, Ghosh B (2013a) Field evaluation and genetic stability assessment of regenerated plants produced via direct shoot organogenesis from leaf explant of an endengered ‘Asthma Plant’ (Tylophora indica) along with their in vitro conservation. Nat Acad Sci Lett 36:551‒562CrossRefGoogle Scholar
  12. Haque SM, Ghosh B (2013b) High frequency microcloning of Aloe vera and their true-to-type conformity by molecular cytogenetic assessment of two years old field growing regenerated plants. Bot Stud 54:46CrossRefPubMedPubMedCentralGoogle Scholar
  13. Haque SM, Ghosh B (2016) High-frequency somatic embryogenesis and artificial seeds for mass production of true-to-type plants in Ledebouria revoluta: an important cardioprotective plant. Plant Cell Tiss Org Cult 127:71‒83Google Scholar
  14. Hazarika BN (2006) Morpho-physiological disorders in in vitro culture plants. Sci Hortic 108:105‒120CrossRefGoogle Scholar
  15. Hoang NN, Kitaya Y, Morishita T, Endo R, Shibuya T (2017) A comparative study on growth and morphology of wasabi plantlets under the influence of the micro-environment in shoot and root zones during photoautotrophic and photomixotrophic micriopropagation. Plant Cell Tiss Org Cult 130:255‒263CrossRefGoogle Scholar
  16. Kadleček P, Tichá I, Haisel D, Čapková V, Schäfer C (2001) Importance of in vitro acclimatization and growth. Plant Sci 161:695‒701Google Scholar
  17. Karkacier M, Ebras M, Uslu MK, Aksu M (2003) Comparison of different extraction and detection methods for sugars using amino-bonded phase HPLC. J Chromatog Sci 41:331‒333CrossRefGoogle Scholar
  18. Karumamkandathil R, Uthum TK, Sankaran S, Unnikrishnan D, Saha T, Nair SS (2015) Genetic and epigenetic uniformity of polyembryony derived multiple seedlings of Hevea brasiliensis. Protoplasma 252:783–796CrossRefPubMedGoogle Scholar
  19. Kositsup B, Kasemsap P, Thanisawanyangkura S, Chairungsee N, Satakhun D, Teerawatanasuk K, Ameglio T, Thaler P (2010) Effect of leaf age and position on light-saturated CO2 assimilation rate, photosynthetic capacity, and stomatal conductance in rubber trees. Photosynthetica 48:67–78CrossRefGoogle Scholar
  20. Kumar K, Rao IU (2012) Morphophysiologicals problems in acclimatization of micropropagated plants in-ex vitro conditions: a reviews. J Orn Hortic Plant 2:271‒283Google Scholar
  21. Li RY, Murthy HN, Kim SK, Paek KY (2001) CO2-enrichment and photosynthetic photon flux affect the growth of in vitro-cultured apple plantlets. J Plant Biol 44:87‒91CrossRefGoogle Scholar
  22. Liu X, Pijut PM (2008) Plant regeneration from in vitro leaves of mature black cherry (Prunus serotina). Plant Cell Tiss Org Cult 94:113‒123CrossRefGoogle Scholar
  23. Loggini B, Scartazza A, Brugnoli E, Navari-Izzo F (1999) Antioxidant defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiol 119:1091‒1099CrossRefGoogle Scholar
  24. Martin KP (2003) Rapid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant. Plant Cell Rep 21:415‒420CrossRefGoogle Scholar
  25. Maxwell K, Johnson GN (2000) Chlorophyll fluorescence-a practical guide. J Exp Bot 51:659‒668CrossRefGoogle Scholar
  26. Mereti M, Grigoriadou K, Nanos GD (2002) Micropropagation of the strawberry tree, Arbutus unedo L. Sci Hortic 93:143‒148CrossRefGoogle Scholar
  27. Morini S, Melai M (2003) CO2 dynamics and growth in photoautotrophic and photomixotrophic apple cultures. Biol Plant 47:167‒172Google Scholar
  28. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  29. Nataraja KN, Jacob J (1999) Clonal differences in photosynthesis in Hevea brasiliensis Müll. Arg Photosyth 36:89‒98Google Scholar
  30. Nor Mayati CH (2015) Effects of zeatin and kinetin on in vitro regeneration of Hevea brasiliensis RRIM 2025. J Rubber Res 18:127‒138Google Scholar
  31. Osório ML, Gonçalves S, Osório J, Romano A (2005) Effects of CO2 concentration on acclimatization and physiological responses of two cultivars of carob tree. Biol Plant 49:161‒167CrossRefGoogle Scholar
  32. Pence VC (2011) Evaluating cost for the in vitro propagation and preservation of endangered plants. In Vitro Cell Dev Biol-Plant 47:176‒187CrossRefGoogle Scholar
  33. Pérez-Jiménez M, López-Pérez AJ, Otálora-Alcón G, Marín-Nicolás D, Piñero MC, del Amor FM (2015) A regime of high CO2 concentration improves the acclimatization process and increases plant quality and survival. Plant Cell Tiss Org Cult 121:547–557CrossRefGoogle Scholar
  34. Pospíšilová J, Haisel D, Synková H, Čatský J, Wilhelmová N, Plzáková Š, Procházková D, Šrámek F (2000) Photosynthetic pigments and gas exchange during ex vitro acclimation of tobacco plants as affected by CO2 supply and abscisic acid. Plant Cell Tiss Org Cult 61:125‒133Google Scholar
  35. Prommee W, Sreenakkiang W, Te-chato S (2014) Somatic embryogenesis and plant regeneration from inner integument of Hevea brasiliensis. In: International conference on rubber (2014ICR), Thaksin University, Phatthalung CampusGoogle Scholar
  36. Rani V, Raina N (2000) Genetic fidelity of organized meristem-derived micropropagated plants: a critical reappraisal. In Vitro Cell Dev Biol‒Plant 36:319‒330CrossRefGoogle Scholar
  37. Reuveni J, Bugbee B (1997) Very high CO2 reduces photosynthesis, dark respiration and yield in wheat. Ann Bot 80:539‒546CrossRefGoogle Scholar
  38. Rolland F, Moore B, Sheen J (2002) Sugar sensing and signaling in plants. Plant Cell 14:S185–S205CrossRefPubMedPubMedCentralGoogle Scholar
  39. Sanguansermsri M, Khamthup P, Meechana K, Wongsawad M, Buddharaksa P (2015) In vitro culture of Hevea brasiliensis (rubber tree) embryo. Naresuan Phayao J 8:155–158Google Scholar
  40. Seon JH, Cui YY, Kozai T, Paek KY (2000) Influence of in vitro growth conditions on photosynthetic competence and survival rate of Rehmannia glutinosa plantlets during acclimatization period. Plant Cell Tiss Org Cult 61:135‒142CrossRefGoogle Scholar
  41. Shin KS, Park SY, Paek KY (2014) Physiological and biochemical changes during acclimatization in a Dorita enopsis hybrid cultivated in different microenvironments in vitro. Environ Exp Bot 100:26‒33CrossRefGoogle Scholar
  42. Smeekens S (2000) Sugar-induced signal transduction in plants. Ann Rev Plant Biol 51:49‒81Google Scholar
  43. Smeekens S, Ma J, Hanson J, Rolland F (2009) Sugar signals and molecular networks controlling plant growth. Curr Opin Plant Biol 13:1‒6Google Scholar
  44. Toler JE, Adelberg JW, Bishop D (2003) Growth and net photosynthetic rates of Hosta ‘Blue Vision’ during acclimatization in bright, natural light with CO2 enrichment. In Vitro Cell Dev Biol-Plant 39:338–342CrossRefGoogle Scholar
  45. Vyas S, Purohit SD (2003) In vitro growth and shoot multiplication of Wrightia tomentosa Roem et Schult in a controlled carbon dioxide environment. Plant Cell Tiss Org Cult 75:283‒286CrossRefGoogle Scholar
  46. Xiao Y, Niu G, Kozai T (2011) Development and application of photoautotrophic micropropagation plant system. Plant Cell Tiss Org Cult 105:149‒158CrossRefGoogle Scholar
  47. Zhou YH, Guo DP, Zhu ZJ, Qian QQ (2005) Effects of in vitro rooting environments and irradiance on growth and photosynthesis of strawberry plantlets during acclimatization. Plant Cell Tiss Org Cult 81:105‒108CrossRefGoogle Scholar
  48. Zhou QN, Jiang ZH, Huang TD, Li WG, Sun AH, Dai XM, Li Z (2010) Plant regeneration via somatic embryogenesis from root explants of Hevea brasiliensis. Afri J Biotechnol 9:8168–8173CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  1. 1.National Center for Genetic Engineering and Biotechnology (BIOTEC)National Science and Technology Development Agency (NSTDA)Pathum ThaniThailand
  2. 2.Chachoengsao Rubber Research CenterRubber Research InstituteChachoengsaoThailand
  3. 3.National Center for Genetic Engineering and Biotechnology (BIOTEC)National Science and Technology Development Agency (NSTDA)Pathum ThaniThailand

Personalised recommendations