Journal of Thrombosis and Thrombolysis

, Volume 48, Issue 3, pp 430–438 | Cite as

Platelet spreading on fibrinogen matrix, a reliable and sensitive marker of platelet functional activity during storage

  • Ehteramolsadat Hosseini
  • Mehran GhasemzadehEmail author
  • Elaheh Azizvakili
  • Pezhman Beshkar


Upon platelet activation, inside-out signals synergistically induced by a variety of agonists and adhesion molecules can enhance the affinity of platelet main integrin, αIIbβ3 to its ligands. Integrin ligation with fibrinogen induces potent signals which develop platelet function including aggregation, release and spreading of which platelet spreading is considered as a major early consequence of αIIbβ3 outside-in signaling. Study presented here evaluated platelet spreading on fibrinogen matrix as a marker of platelet activation during storage. PRP-platelet concentrates were subjected to flowcytometry analysis and the expression levels of P-selectin, CD61, GPIbα and active conformation of the αIIbβ3 (PAC-1 binding) were examined on day 0, 1, 3 and 5 post-storage. Concurrently platelet adhesion and spreading on fibrinogen matrix, glucose concentration and LDH activity were also determined at the same intervals. Results showed significant decreases in platelet spreading on fibrinogen matrix during storage. Spreading was dominant pattern of adhesion till the first day of storage. In 3 day-stored platelets, filopodial or lamellipodial formation was dominant event whereas 5 day-stored platelets simply adhered to fibrinogen with less protrusion formation and partially failed to spread. Compared to simple adhesion, reduction of platelet spreading was also more significantly correlated with the usual markers of platelet storage lesion including P-selectin (r = − 0.88; p < 0.0001) and GPIbα expression (r = 0.76; p = 0.0001), PAC-1 binding (r = 0.66; p = 0.001), glucose concentration and LDH activity. Taken together, we introduced platelet spreading on fibrinogen matrix as a reliable and sensitive marker of platelets functional activity during storage. As a valid marker which is directly and obviously relevant to the platelet functional capacities, the rapid reduction of platelet spreading during storage overshadows other markers of platelet storage lesion while raising serious question about the quality of 5 day-stored platelets.


Adhesion Fibrinogen Integrin alpha IIb beta 3 (αIIbβ3Platelet spreading Platelet storage lesion Platelet transfusion Thrombosis 



This work was part of Dr. Ghasemzadeh and Dr. Hosseini’s approved projects (Nos. 1394-01-33-1861 and 1396-06-33-2036) which were supported by Iranian blood transfusion organization and High Institute for Research and Education in Transfusion Medicine in Iran, also approved by local ethic committee. The authors declare no conflict of interests.

Author contributions

MG supervised and designed the study, did the experiments, analyzed the data and wrote the paper. EH did some experiments, helped with analyzing data and co-wrote the paper. EA did some experiments as a part of her MS project. PB did some experiments.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ehteramolsadat Hosseini
    • 1
  • Mehran Ghasemzadeh
    • 1
    • 2
    • 4
    Email author
  • Elaheh Azizvakili
    • 1
  • Pezhman Beshkar
    • 1
    • 3
  1. 1.Blood Transfusion Research CenterHigh Institute for Research and Education in Transfusion MedicineTehranIran
  2. 2.Australian Centre for Blood DiseasesMonash UniversityMelbourneAustralia
  3. 3.Cellular and Molecular Research Centre, Basic Health Science InstituteShahrekord University of Medical ScienceSharekordIran
  4. 4.Blood Transfusion Research CentreHigh Institute for Research and Education in Transfusion MedicineTehranIran

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