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Russian Chemical Bulletin

, Volume 68, Issue 5, pp 1088–1095 | Cite as

Immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nanoparticles

  • A. M. DeminEmail author
  • M. S. Valova
  • A. G. Pershina
  • V. P. Krasnov
Full Article

Abstract

The immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nano particles (MNPs), preliminarily functionalized with 3-aminopropylsilane and N-(phosphonomethyl) iminodiacetic acid (PMIDA), was studied. Protein binding to MNPs depending on the nature of the surface functional groups, the medium, and the presence of a coupling agent, namely, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), was studied using fluorimetry, UV and IR spectroscopy. It was shown that protein immobilization can occur by both non-covalent and covalent binding with surface groups of MNPs. The best results (up to 150 μg of TagGFP2 per 1 mg of MNPs) were obtained for MNPs preliminarily functionalized with PMIDA and using EDC. The results of this study can be used for the design of imaging agents having both magnetic and optical properties to be applied in biomedicine.

Key words

magnetic nanoparticles TagGFP2 fluorimetry UV spectroscopy 

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Copyright information

© Springer Science+Business Media, Inc. 2019

Authors and Affiliations

  • A. M. Demin
    • 1
    Email author
  • M. S. Valova
    • 1
  • A. G. Pershina
    • 2
    • 3
  • V. P. Krasnov
    • 1
  1. 1.I. Ya. Postovsky Institute of Organic SynthesisUral Branch of the Russian Academy of SciencesEkaterinburgRussian Federation
  2. 2.Siberian State Medical UniversityTomskRussian Federation
  3. 3.National Research Tomsk Polytechnic UniversityTomskRussian Federation

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