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Russian Chemical Bulletin

, Volume 68, Issue 1, pp 163–167 | Cite as

The K5 capsular polysaccharide of the bacterium Acinetobacter baumannii SDF with the same K unit containing Leg5Ac7Ac as the K7 capsular polysaccharide but a different linkage between the K units

  • N. P. Arbatsky
  • J. J. Kenyon
  • A. S. Shashkov
  • M. M. Shneider
  • A. V. Popova
  • N. A. Kalinchuk
  • R. M. Hall
  • Yu. A. KnirelEmail author
Full Articles
  • 7 Downloads

Abstract

The K5 capsular polysaccharide (CPS) was isolated from the bacterium Acinetobacter baumannii (A. baumannii) SDF and studied by 1D and 2D 1H and 13C NMR spectroscopy before and after O-deacetylation and partial acid hydrolysis. The CPS was found to be composed of branched tetrasaccharide repeats (K units) containing 5,7-diacetamido-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic acid (Leg5Ac7Ac). The KL5 capsule biosynthesis gene cluster at the K locus is consistent with the composition and structure of the CPS. KL5 is almost identical to the KL7 gene ulosonic acid (Leg5Ac7Ac A. baumannii LUH5533 that has been characterized earlier, and differs only in the gene for Wzy polymerase that is responsible for the formation of the linkage between the K units. The K5 CPS from strain SDF and the K7 CPS from strain LUH5533 have the same K-unit structure but a different linkage between the K units, which is formed by distinct Wzy polymerases. As opposite to Leg5Ac7Ac in the K7 CPS, this monosaccharide is O-acetylated at position 4 in the K5 CPS. No acetyltransferase for this modification of the K5 CPS is present in the KL5 gene cluster, and hence it is encoded by a gene located elsewhere in the genome.

Key words

Acinetobacter baumannii capsular polysaccharide legionaminic acid KL gene locus Wzy polymerase gene polymorphism 

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Copyright information

© Springer Science+Business Media, Inc. 2019

Authors and Affiliations

  • N. P. Arbatsky
    • 1
  • J. J. Kenyon
    • 2
  • A. S. Shashkov
    • 1
  • M. M. Shneider
    • 3
  • A. V. Popova
    • 4
    • 5
  • N. A. Kalinchuk
    • 1
  • R. M. Hall
    • 6
  • Yu. A. Knirel
    • 1
    Email author
  1. 1.N. D. Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussian Federation
  2. 2.Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of HealthQueensland University of TechnologyBrisbaneAustralia
  3. 3.M. M. Shemyakin–Yu. A. Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussian Federation
  4. 4.Moscow Institute of Physics and Technology9 Institutskii per.Russian Federation
  5. 5.State Research Center for Applied Microbiology and BiotechnologyObolenskRussian Federation
  6. 6.School of Life and Environmental SciencesThe University of SydneySydneyAustralia

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