Russian Chemical Bulletin

, Volume 67, Issue 4, pp 762–767 | Cite as

Detection of natural antibodies to endogenous bioregulators for the diagnostics of the functional state of the body

  • M. A. Myagkova
  • S. N. Petrochenko
  • V. S. MorozovaEmail author
Full Article


An immunological method for the detection of natural antibodies (NAb) to endogenous bioregulators (β-endorphin, orphanin, angiotensin, serotonin, dopamine, histamine, γ-aminobutyric acid (GABA), and glutamate) has been developed for the evaluation of the functional state of the human body. The antibody level was determined at various stages of exercises during the period of sport training. A relationship was established between the change in the NAb level and the increased training load or the duration of exercises. The difference in the NAb levels for athletes specializing in Greco-Roman wrestling, soccer, and hockey was established. The initially high values for orphanin, GABA, and glutamate were found in hockey players, while these indicators in wrestlers were dynamically increased during the training process. Immunological indicators for soccer players were close to the physiologically normal ones and were similar to the parameters of people engaged in sports and recreational gymnastics. It was demonstrated that the diagnostic tests for the detection of natural antibodies to the listed biomolecules could have a practical application to evaluate the effectiveness of the functional state of the human body.

Key words

adaptive capacity γ-aminobutyric acid glutamic acid β-endorphin orphanin dopamine enzyme-linked immunosorbent assay (ELISA) training load 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • M. A. Myagkova
    • 1
  • S. N. Petrochenko
    • 1
  • V. S. Morozova
    • 1
    Email author
  1. 1.Institute of Physiologically Active Compounds of the Russian Academy of SciencesChernogolovka, Moscow Region, Russian FederationRussia

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