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Russian Chemical Bulletin

, Volume 67, Issue 4, pp 747–756 | Cite as

Development of a polymer system for the delivery of daunorubicin to tumor cells to overcome drug resistance

  • E. D. Nikolskaya
  • M. R. Faustova
  • M. D. Mollaev
  • O. A. Zhunina
  • M. B. Sokol
  • N. G. Yabbarov
  • N. V. Gukasova
  • A. V. Lobanov
  • V. I. Shvets
  • E. S. Severin
Full Article
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Abstract

Method for the synthesis of polymeric nanoparticles (NP) with encapsulated daunorubicin (DNR) was developed on the basis of double emulsion solvent evaporation technique using biodegradable poly(lactide-co-glycolide) (PLGA), which is aimed at customization of pharmacokinetic properties of the preparation, enhanced accumulation of DNR in tumor cells and prolongation of its action. The obtained polymer nanoparticles (DNR-PLGA) had average size ranging around 138±36 nm, with zeta-potential of –25.3 mV and the polydispersity index (PDI) of 0.072. The release kinetics of DNR from polymer nanoparticles at pH 7.4 and 5.0 has been studied. In vitro studies showed similar specific activity of DNR- PLGA in K562 and MCF-7 cancer cell lines together with an increase in activity in K562 Adr and MCF-7 Adr cell lines, which are anthracycline resistant, by 1.6 and 3.4 times. The study demonstrated the efficacy of the developed PLGA-based DNR delivery system in the improvement of antitumor effect of DNR, overcoming multidrug resistance in cancer cells, and also in the decrease in nonspecific toxicity of the preparation.

Key words

daunorubicin (DNR) poly(lactic-co-glycolic acid) (PLGA) polymeric nanoparticles (NP) release kinetics multidrug resistance (MDR) antitumor activity 

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • E. D. Nikolskaya
    • 1
  • M. R. Faustova
    • 1
  • M. D. Mollaev
    • 1
  • O. A. Zhunina
    • 2
  • M. B. Sokol
    • 2
  • N. G. Yabbarov
    • 2
  • N. V. Gukasova
    • 3
  • A. V. Lobanov
    • 4
  • V. I. Shvets
    • 1
  • E. S. Severin
    • 2
  1. 1.Moscow Technological UniversityM. V. Lomonosov Institute for Fine Chemical TechnologiesMoscow, Russian FederationRussia
  2. 2.Russian Research Center for Molecular Diagnostics and TherapyMoscow, Russian FederationRussia
  3. 3.National Research Center Kurchatov InstituteMoscow, Russian FederationRussia
  4. 4.N. N. Semenov Institute of Chemical PhysicsRussian Academy of SciencesMoscow, Russian FederationRussia

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