Research on Chemical Intermediates

, Volume 45, Issue 1, pp 33–49 | Cite as

Antioxidant and cytotoxic activity of lichens collected from Bidoup Nui Ba National Park, Vietnam

  • Thi Thu Hoai Nguyen
  • Minh Hiep Dinh
  • Hoang Thanh Chi
  • San-Lang WangEmail author
  • QuangVinh Nguyen
  • Trung Dzung Tran
  • Anh Dzung NguyenEmail author


Lichens were some of the earliest colonizers of terrestrial habitats on Earth. They represent a unique symbiont between fungi (mycobionts) and algae and/or cyanobacteria (photobionts). Lichens have been used as a cure for diabetes, coughs, pulmonary tuberculosis, wound healing, and dermatological diseases. The aim of this study is to investigate the in vitro antioxidant and cytotoxic activities of methanol lichen extracts. Fourteen lichen species from Bidoup Nui Ba National Park were identified according to their morphology and anatomical and chemical characteristics. The antioxidant activity of the methanol lichen extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay. The results showed that, of the lichens tested, L5 had the highest free radical activity with 50% inhibitory concentration (IC50) of 59.9 ± 4.65 mg mL−1. The methanol extract of L5 also showed the highest total flavonoid and polyphenol contents. In a cytotoxic assay, it was observed that the methanol extract of U38.1 exhibited high cytotoxic effect against MCF-7 cells, with IC50 of 34.27 ± 1.25 mg mL−1. The tested lichen extracts were also found to have slight cytotoxic effect on fibroblasts at screening concentration of 100 µg mL−1. All of the extracts were found to possess different cytotoxic activities against MO-91 cells, with IC50 values ranging from 10.50 ± 1.56 to over 50 mg mL−1. All of the extracts except U38.1 induced normal peripheral blood mononuclear cell (PBMC) proliferation, especially after 48 h of treatment at 25 µg/mL.


Lichen Methanol extracts Cytotoxicity MCF-7 MO-91 Fibroblast Peripheral blood mononuclear cells 



We would like to express our thanks to the Institute of Biotechnology and Environment, Tay Nguyen University; Department of Genetics, Faculty of Biology and Biotechnology, University of Science, Vietnam National University, Ho Chi Minh City; and Korean Lichen Research Institute, Sunchon National University, Korea. This work was supported in part by a grant from the Ministry of Science and Technology, Taiwan (MOST 102-2313-B-032-001-MY3).


  1. 1.
    J. Kumar, P. Dhar, A.B. Tayade, D. Gupta, O.P. Chaurasia, D.K. Upreti, R. Arora, R.B. Srivastava, PLoS ONE 9, 1 (2014)Google Scholar
  2. 2.
    M. Kosanić, B. Ranković, J. Sci. 32, 65–72 (2010)Google Scholar
  3. 3.
    B.C. Behera, N. Verma, A. Sonone, U. Makhija, J Biotechnol. Lett. 27, 991 (2005)CrossRefGoogle Scholar
  4. 4.
    J. Boustie, M. Grube, Plant Genet. Resour. 3, 273 (2005)CrossRefGoogle Scholar
  5. 5.
    Y.Q. Liu, X.Y. Hu, T. Lu, Y.N. Cheng, C.Y.F. Young, H.Q. Yuan, H.X. Lou, PLoS ONE 7, 1 (2012)Google Scholar
  6. 6.
    K. Müller, Appl. Microbiol. Biot. 56, 9 (2001)CrossRefGoogle Scholar
  7. 7.
    N.T. Manojlovic, P.J. Vasiljevic, P.Z. Maskovic, M. Juskovic, G.B. Dusanovic, Hindawi Publ. Corp. 2012, 1 (2012)Google Scholar
  8. 8.
    G. Shrestha, L.L.S. Clair, Phytochem. Rev. 12, 229 (2013)CrossRefGoogle Scholar
  9. 9.
    F. Brisdelli, M. Perilli, D. Sellitri, M. Piovano, J.A. Garbarino, M. Nicoletti, A. Bozzi, G. Amicosante, G. Celenza, Phytother Res. 27, 431 (2013)CrossRefGoogle Scholar
  10. 10.
    B. Ranković, M. M. Kosanić, T. P Stanojković, BMC Complem. Altern. Med. 11, 1 (2011)Google Scholar
  11. 11.
    N. Verma, B.C. Behera, U. Makhija, Appl. Biochem. Biotechnol. 151, 167 (2008)CrossRefGoogle Scholar
  12. 12.
    U. Jayalal, S. Joshi, S.O. Oh, J.A. Kim, Y.J. Koh, F. Crian, J.S. Hur, Mycobiology 42, 6 (2013)CrossRefGoogle Scholar
  13. 13.
    D. Triggiani, D. Ceccarelli, A. Tiezzi, T. Pisani, S. Munzi, C. Gaggi, S. Loppi, Biologia 64, 59 (2009)CrossRefGoogle Scholar
  14. 14.
    U. Jayalal, N.T. Thuy, N.A. Dzung, J.S. Hur, Mycotaxon 124, 51 (2013)CrossRefGoogle Scholar
  15. 15.
    S. Joshi, U. Jayalal, N.T. Thuy, N.A. Dzung, J.S. Hur, The Lichenologist 45, 1 (2013)CrossRefGoogle Scholar
  16. 16.
    S. Joshi, U. Jayalal, N.T. Thuy, N.A. Dzung, J.S. Hur, Mycotaxon 123, 479 (2013)CrossRefGoogle Scholar
  17. 17.
    S. Joshi, N.T. Thuy, N.A. Dzung, J.S. Hur, Mycotaxon 124, 309 (2013)CrossRefGoogle Scholar
  18. 18.
    S. Joshi, N.T. Thuy, N.A. Dzung, J.S. Hur, Mycotaxon 125, 69–80 (2013)CrossRefGoogle Scholar
  19. 19.
    S. Joshi, N.T. Thuy, N.A. Dzung, J.S. Hur, Mycobiology 42, 17 (2014)CrossRefGoogle Scholar
  20. 20.
    S. Joshi, D. K. Upreti, S.O. Oh, · N. T. Thuy, N. A. Dzung, J. S. Hur, Mycotaxon. 130, 329 (2015)Google Scholar
  21. 21.
    N.T. Thuy, N.A. Dzung, J.S. Hur, Taiwania 55, 402 (2010)Google Scholar
  22. 22.
    N.T. Thuy, N.A. Dzung, J.S. Hur, Mycotaxon 117, 93 (2011)CrossRefGoogle Scholar
  23. 23.
    J.A. Elix, K.D. Ernst-Russell, A Catalogue of Standardized Thin Layer Chromatographic Data and Biosynthetic Relationships for Lichen Substances, 2nd edn. (Australian National University, Canberra, 1993)Google Scholar
  24. 24.
    A. Orange, P.W. James, F.J. White, Microchemical Methods for the Identification of Lichens. British Lichen Society, London, UK, 101 (2001)Google Scholar
  25. 25.
    U. Jayalal, P.K. Divakar, S. Joshi, S.O. Oh, Y.J. Koh, J.S. Hur, Mycobiology 41, 25 (2013)CrossRefGoogle Scholar
  26. 26.
    C.K. Lin, Coll. Res. 26, 37 (2013)Google Scholar
  27. 27.
    Y. Ohmura, Mem. Natl. Mus. Nat. Sci. 48, 91 (2012)Google Scholar
  28. 28.
    T. Randlane, T. TõRRa, A. Saag, L. Saag, Bibliotheca Lichenologica 100, 419 (2009)Google Scholar
  29. 29.
    H. Sipman, Mycotaxon 1, 235 (1986)Google Scholar
  30. 30.
    J. Zhishen, T. Mengcheng, W. Jianming, J. Food Chem. 64, 555 (1999)CrossRefGoogle Scholar
  31. 31.
    B.C. Sharma, S. Kalikotay, IOSR-PHR. 2, 54 (2012)Google Scholar
  32. 32.
    C.M. Liyana-Pathirana, F. Shahidi, J. Agric. Food Chem. 53, 2433 (2005)CrossRefGoogle Scholar
  33. 33.
    B. Ranković, M. Kosanić, T. Stanojković, P. Vasiljević, N. Manojlović, Int. J. Mol. Sci. 13, 11 (2011)Google Scholar
  34. 34.
    S.Ç. Kasimoğullari, S. Oran, F. Ari, E. Ulukaya, N. Aztopal, M. Sarimahmut, Ş. Öztürk, Turk. J. Biol. 38, 940 (2014)CrossRefGoogle Scholar
  35. 35.
    N. Manojlovic, B. Rankovi, M. Kosani, P. Vasiljevi, T. Stanojkovi, Phytomedicine 19, 1166 (2012)CrossRefGoogle Scholar
  36. 36.
    N. T.Manojlovic, P. J. Vasiljevic, P. Z. Maskovic, M. Juskovic, G. B. Dusanovic, eCAM. 2012, 1 (2012)Google Scholar
  37. 37.
    M. Bacˇkorová, R. Jendz ˇelovsky´, M. Kello, M. Bac ˇkor, J. Mikeš, P. Fedorocˇko, Toxicol. In Vitro 26, 462 (2012)Google Scholar
  38. 38.
    A. Itharat, P.J. Houghton, E. Eno-Amooquaye, P.J Burke, J.H. Sampson, A. Raman, J. Ethnopharmacology 90, 33 (2004)Google Scholar
  39. 39.
    S.M.K. Swamy, B.K.H. Tan, J. Ethnopharm. 70, 1 (2000)CrossRefGoogle Scholar
  40. 40.
    B. Ranković, M. Kosanić, T. Stanojković, P. Vasiljević, N. Manojlović, Int. J. Mol. Sci. 13, 14707 (2012)CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Thi Thu Hoai Nguyen
    • 1
  • Minh Hiep Dinh
    • 2
  • Hoang Thanh Chi
    • 3
  • San-Lang Wang
    • 4
    Email author
  • QuangVinh Nguyen
    • 5
  • Trung Dzung Tran
    • 5
  • Anh Dzung Nguyen
    • 5
    Email author
  1. 1.Department of Experimental Biology, Faculty of Natural Science and TechnologyTay Nguyen UniversityBuon Ma Thuot CityVietnam
  2. 2.Hi-tech Agricultural ParkHo Chi Minh CityVietnam
  3. 3.Biotechnology Center of Ho Chi Minh CityHo Chi Minh CityVietnam
  4. 4.Life Science Center/Department of ChemistryTamkang UniversityNew Taipei CityTaiwan
  5. 5.Institute of Biotechnology and EnvironmentTay Nguyen UniversityBuon Ma Thuot CityVietnam

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