High catalytic activity of gold nanoparticle-templated, tyrosine-rich peptide self-assemblies for 3,3′,5,5′-tetramethylbenzidine oxidation in the absence of hydrogen peroxide
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In this study, gold nanoparticle-templated, tyrosine-rich peptide (YC7@AuNPs) self-assemblies were examined as peroxidase enzyme–mimic catalysts for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) and its activity was monitored by changing the reaction conditions such as pH, temperature, initial TMB concentration, and catalyst (Au) loading amount. The YC7@AuNPs catalysts were active for TMB oxidation even in the absence of hydrogen peroxide. Michaelis–Menten kinetic analysis showed that the YC7@AuNPs catalysts without hydrogen peroxide have activities comparable to other peroxidase–mimic catalysts using hydrogen peroxide in terms of the Michaelis constant.
KeywordsGold nanoparticles YC7 peptide 3,3′,5,5′-tetramethylbenzidine (TMB) Peroxidase Enzyme-mimic catalyst
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science & ICT(MSIT) (NRF-2016R1A5A1009592).
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