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Reaction Kinetics, Mechanisms and Catalysis

, Volume 128, Issue 1, pp 349–359 | Cite as

High catalytic activity of gold nanoparticle-templated, tyrosine-rich peptide self-assemblies for 3,3′,5,5′-tetramethylbenzidine oxidation in the absence of hydrogen peroxide

  • Namhun Lee
  • Sang-Myung Lee
  • Dae-Won LeeEmail author
Article
  • 33 Downloads

Abstract

In this study, gold nanoparticle-templated, tyrosine-rich peptide (YC7@AuNPs) self-assemblies were examined as peroxidase enzyme–mimic catalysts for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) and its activity was monitored by changing the reaction conditions such as pH, temperature, initial TMB concentration, and catalyst (Au) loading amount. The YC7@AuNPs catalysts were active for TMB oxidation even in the absence of hydrogen peroxide. Michaelis–Menten kinetic analysis showed that the YC7@AuNPs catalysts without hydrogen peroxide have activities comparable to other peroxidase–mimic catalysts using hydrogen peroxide in terms of the Michaelis constant.

Keywords

Gold nanoparticles YC7 peptide 3,3′,5,5′-tetramethylbenzidine (TMB) Peroxidase Enzyme-mimic catalyst 

Notes

Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science & ICT(MSIT) (NRF-2016R1A5A1009592).

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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2019

Authors and Affiliations

  1. 1.Department of Chemical Engineering, Division of Chemical and Biological EngineeringKangwon National UniversityChuncheonRepublic of Korea
  2. 2.Division of Applied RIKorea Institute of Radiological and Medical SciencesSeoulRepublic of Korea

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