Quantitative Analysis of the Biologically Active Compounds Present in Leaves of Mexican Sweet Potato Accessions: Phenols, Flavonoids, Anthocyanins, 3,4,5-Tri-Caffeoylquinic Acid and 4-Feruloyl-5-Caffeoylquinic Acid Original Paper First Online: 11 November 2019 Abstract
Sweet potato is one of the oldest crops cultivated in Mexico, and Mesoamerica is considered as a region with the greatest diversity of this species. Therefore, the present study focused on the evaluation of biologically active compounds, such as caffeoylquinic acid derivatives and flavonoid compounds, in sweet potato leaves of 200 accessions of the main producing regions of Mexico. The analysis of total phenol content (TPC) showed a great variability of concentrations among the examined accessions (54.41 to 284.64 mgTPC/g DW). Likewise, total flavonoid content (TFC) was determined and ranged from 10.01 to 40.17 mgTFC /g DW. Finally, total anthocyanin content (TAC) was evaluated and concentrations obtained varied from 0.05 to 0.98 mgTAC/g DW. Additionally, HPLC analysis of all 200 accessions demonstrated the presence of caffeic acid (CA), 5-caffeoylquinic acid (5-CQA), three isomers of di-caffeoylquinic acid (di-CCA) and 4-feruloyl-5-caffeoylquinic acid (4F-5CQA) in all test samples. Only 21 accessions tested showed the quantitative amount of 3,4,5-tri-caffeoylquinic acid (3,4,5-tri-CQA) with concentrations ranging from 44.73 to 193.22 mg/100 g DW and high content of 4F-5CQA (139.46 to 419.99 mg/100 g DW). The gathered data indicate that leaves of Mexican sweet potatoes are a promising source of phenolic compounds with remarkable nutraceutical potential.
Keywords Sweet potato leaves Phenolic compounds 3,4,5-tri-caffeoylquinic acid 4-feruloyl-5-caffeoylquinic acid Abbreviations CA
Ipomoea batatas 5-CQA
Total anthocyanin content
Total flavonoid content
Total phenol content
Electronic supplementary material
The online version of this article (
) contains supplementary material, which is available to authorized users. https://doi.org/10.1007/s11130-019-00774-2 Notes Acknowledgments
The CONACYT doctoral scholarship of Andrea Torres (scholarship number: 308249); the technical support of Margarita Guzman; project PAPIIT-UNAM-IT202318.
Compliance with Ethical Standards Conflict of Interest
The authors declare no conflict of interest.
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Mohanraj R, Sivasankar S (2014) Sweet potato (
[L.] Lam) - a valuable medicinal food: a review. J Med Food 17:733–741.
https://doi.org/10.1089/jmf.2013.2818 CrossRef PubMed Google Scholar
Mu T, Sun H, Zhang M, Wang C (2017) Chlorogenic acids from sweet potato. Sweet Potato Processing Technology. Elsevier, pp 357–403
Islam I, Shaikh AU, Shahidul IM (2009) Antioxidative and antimutagenic potentials of phytochemicals from
(L.) Lam. Int J Cancer Res 5:83–94.
https://doi.org/10.3923/ijcr.2009.83.94 CrossRef Google Scholar
Zhang L, Tu Z, Wang H et al (2015) Comparison of different methods for extracting polyphenols from I
leaves, and identification of antioxidant constituents by HPLC-QTOF-MS2. Food Res Int 70:101–109.
https://doi.org/10.1016/j.foodres.2015.01.012 CrossRef Google Scholar
Zheng W, Clifford MN (2008) Profiling the chlorogenic acids of sweet potato (I
) from China. Food Chem 106:147–152.
https://doi.org/10.1016/j.foodchem.2007.05.053 CrossRef Google Scholar
Pereira A dos Santos, Pereira AF de M, Trugo L, Neto FR de A (2003) Distribution of quinic acid derivatives and other phenolic compounds in Brazilian propolis. Zeitschrift fur Naturforsch C58:590–593.
https://doi.org/10.1515/znc-2003-7-824 CrossRef Google Scholar
Mahmood N, Moore PS, De Tommasi N et al (1993) Inhibition of HIV infection by caffeoylquinic acid derivatives. Antivir Chem Chemother 4:235–240.
https://doi.org/10.1177/095632029300400406 CrossRef Google Scholar
Heyman HM, Senejoux F, Seibert I, Klimkait T, Maharaj VJ, Meyer JJ (2015) Identification of anti-HIV active dicaffeoylquinic- and tricaffeoylquinic acids in
H elichrysum populifolium
by NMR-based metabolomic guided fractionation. Fitoterapia 103:155–164.
https://doi.org/10.1016/j.fitote.2015.03.024 CrossRef PubMed Google Scholar
Garg SK (2016) Green coffee bean. Nutraceuticals Effic Saf Toxic:653–667.
https://doi.org/10.1016/B978-0-12-802147-7.00047-4 CrossRef Google Scholar
Yoshimoto M, Yahara S, Okuno S et al (2002) Antimutagenicity of mono-, di-, and tricaffeoylquinic acid derivatives isolated from sweetpotato (
L.) leaf. Biosci Biotechnol Biochem 66:2336–2341.
https://doi.org/10.1271/bbb.66.2336 CrossRef PubMed Google Scholar
Kurata R, Yahara S, Yamakawa O, Yoshimoto M (2011) Simple high-yield purification of 3,4,5-tri-O-caffeoylquinic acid from sweetpotato (
s L.) leaf and its inhibitory effects on aldose reductase. Food Sci Technol Res 17:87–92.
https://doi.org/10.3136/fstr.17.87 CrossRef Google Scholar
Miyamae Y, Kurisu M, Han J, Isoda H, Shigemori H (2011) Structure–activity relationship of caffeoylquinic acids on the accelerating activity on ATP production. Chem Pharm Bull 59:502–507.
https://doi.org/10.1248/cpb.59.502 CrossRef PubMed Google Scholar
Lee CS, Lee SA, Kim YJ et al (2011) 3,4,5-Tticaffeoylquinic acid inhibits tumor necrosis factor-α- stimulated production of inflammatory mediators in keratinocytes via suppression of Akt- and NF-κB-pathways. Int Immunopharmacol 11:1715–1723.
https://doi.org/10.1016/j.intimp.2011.06.003 CrossRef PubMed Google Scholar
Jeng TL, Lai CC, Liao TC et al (2015) Effects of drying on caffeoylquinic acid derivative content and antioxidant capacity of sweet potato leaves. J Food Drug Anal 23:701–708.
https://doi.org/10.1016/j.jfda.2014.07.002 CrossRef PubMed Google Scholar
Islam MS, Yoshimoto M, Terahara N, Yamakawa O (2002) Anthocyanin compositions in sweetpotato (
L.) leaves. Biosci Biotechnol Biochem 66:2483–2486.
https://doi.org/10.1271/bbb.66.2483 CrossRef PubMed Google Scholar
Kano M, Takayanagi T, Harada K et al (2005) Antioxidative activity of anthocyanins from purple sweet potato,
cultivar Ayamurasaki. Biosci Biotechnol Biochem 69:979–988.
https://doi.org/10.1271/bbb.69.979 CrossRef PubMed Google Scholar
Vishnu VR, Renjith RS, Mukherjee A, et al (2019) Comparative study on the chemical structure and
antiproliferative activity of anthocyanins in purple root tubers and leaves of sweet potato (
). J Agric Food Chem 67:2467-2475.
https://doi.org/10.1021/acs.jafc.8b05473 CrossRef Google Scholar
Zhang ZC, Su GH, Luo CL et al (2015) Effects of anthocyanins from purple sweet potato (
L. cultivar Eshu no. 8) on the serum uric acid level and xanthine oxidase activity in hyperuricemic mice. Food Funct 6:3045–3055.
https://doi.org/10.1039/c5fo00499c CrossRef PubMed Google Scholar
Taga MS, Miller EE, Pratt DE (1984) Chia seeds as a source of natural lipid antioxidants. J Am Oil Chem Soc 61:928–931.https://doi.org//10.1007/BF02542169
CrossRef Google Scholar
Chang C-C, Yang M-H, Wen H-M, Chern J-C (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178–182
Giusti MM, Wrolstad RE (2001) Characterization and measurement of anthocyanins by UV-visible spectroscopy. Curr Protoc Food Anal Chem 00:F1.2.1–F1.2.13.
https://doi.org/10.1002/0471142913.faf0102s00 CrossRef Google Scholar
Wianowska D, Gil M (2019) Recent advances in extraction and analysis procedures of natural chlorogenic acids. Phytochem Rev 18:273–302.
https://doi.org/10.1007/s11101-018-9592-y CrossRef Google Scholar
Song J, Li D, Liu C, Zhang Y (2011) Optimized microwave-assisted extraction of total phenolics (TP) from
leaves and its antioxidant activity. Innov Food Sci Emerg Technol 12:282–287.
https://doi.org/10.1016/j.ifset.2011.03.001 CrossRef Google Scholar
Frati A, Antonini E, Ninfali P (2016) Industrial freezing, cooking, and storage differently affect antioxidant nutrients in vegetables. Fruits, Vegetables, and Herbs. Elsevier, pp 23–39
Salgado P, Favarin J, Leandro R, Filho O (2008) Total phenol concentrations in coffee tree. Sci Agric (Piracicaba, Braz) 65:354–359.
https://doi.org/10.1590/S0103-90162008000400005 CrossRef Google Scholar
Zamora-Ros R, Knaze V, Rothwell JA, et al (2016) Dietary polyphenol intake in europe: the European prospective investigation into cancer and nutrition (EPIC) study. Eur J Nutr 55:1359–1375.
https://doi.org/10.1007/s00394-015-0950-x CrossRef PubMed Google Scholar
Su X, Griffin J, Xu J, Ouyang P, Zhao Z, Wang W (2019) Identification and quantification of anthocyanins in purple-fleshed sweet potato leaves. Heliyon 5:e01964.
https://doi.org/10.1016/j.heliyon.2019.e01964 CrossRef PubMed PubMedCentral Google Scholar
Jung JK, Lee SU, Kozukue N et al (2011) Distribution of phenolic compounds and antioxidative activities in parts of sweet potato (
L.) plants and in home processed roots. J Food Compos Anal 24:29–37.
https://doi.org/10.1016/j.jfca.2010.03.025 CrossRef Google Scholar
Islam S, Yoshimoto M, Yahara S et al (2002) Identification and characterization of foliar polyphenolic composition in sweetpotato (
L.) genotypes. J Agric Food Chem 50:3718–3722.
https://doi.org/10.1021/jf020120l CrossRef PubMed Google Scholar Copyright information
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