Plant Foods for Human Nutrition

, Volume 74, Issue 4, pp 508–517 | Cite as

Chemical and Nutritional Evaluation of Protein-Rich Ingredients Obtained through a Technological Process from Yellow Lupin Seeds (Lupinus luteus)

  • César Burgos-DíazEmail author
  • Mauricio Opazo-Navarrete
  • Traudy Wandersleben
  • Monserrat Soto-Añual
  • Tamara Barahona
  • Mariela Bustamante
Original Paper


In recent years, interest in plant-based proteins has been rising due to ethical and sustainability issues. In this context, the production of protein concentrates and isolates from new plant sources have increased enormously because of their nutritional and techno-functional properties. Therefore, this work describes a pilot process for obtaining protein-rich ingredients from a yellow lupin variety (Lupinus luteus) developed by the Agriaquaculture Nutritional Genomic Center (CGNA). A protein alkaline solubilisation followed by isoelectric precipitation was used as a method for obtaining a protein concentrate (LPC) and isolate (LPI) with 75 and 95% protein, respectively. The changes in the protein quality and chemical composition during the protein concentration process from lupin flour were evaluated. Thus, nutritional parameters such as the amino acids (AAs) profile, essential amino acid index (EAAI), chemical score (CS), the protein efficiency ratio (PER), and digestible indispensable amino acid score (DIAAS) were determined to evaluate the nutritional quality of LPC and LPI. The facile and scalable protein isolation method without a defatting process proposed in this study showed a great protein separation efficiency (PSE), while the amino acids profile was not affected during both the concentration as well as the isolation of proteins. In addition, SDS-PAGE showed that both LPC and LPI mainly contained the high-molecular-weight proteins α and β-conglutins. Both LPC and LPI had a balanced amino acids profile, and arginine was the most predominant amino acid. These results are useful for increasing the use of lupin based-protein ingredients as a potential functional ingredient in the food industry.


Lupinus luteus Protein isolate Rich-protein Amino acids Nutritional properties 



Amino acid


Amino acids


Crude protein


Chemical score


Digestible indispensable amino acid score


Essential amino acid


Essential amino acids


Essential amino acid index


Lupin flour


Lupin protein concentrate


Lupin protein isolate


Nitrogen-free extract


Protein efficiency ratio


Protein separation efficiency


Sodium dodecyl sulphate polyacrylamide gel electrophoresis


Standardised ileal digestibility



This research was supported by CONICYT through project N°R16F1002 (Programa de Fortalecimiento de Centros Regionales) and CONICYT-FONDEF/ ID18I10093. We acknowledge the Regional Government from La Araucanía Region (Chile).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.


  1. 1.
    Lee V (2019) Food Ten Trends for 2019. Accessed 5 August 2019
  2. 2.
    Gollnow F, Lakes T (2014) Policy change, land use, and agriculture: the case of soy production and cattle ranching in Brazil, 2001-2002. Appl Geogr 55:203–211CrossRefGoogle Scholar
  3. 3.
    Schindler S, Wittig M, Zelena K, Krings U, Bez J, Eisner P, Berger RG (2011) Lactic fermentation to improve the aroma of protein extracts of sweet lupin (Lupinus angustifolius). Food Chem 128:330–337CrossRefGoogle Scholar
  4. 4.
    Mattila P, Mäkinen S, Eurola M, Jalava T, Pihlava JM, Hellström J, Pihlanto A (2018) Nutritional value of commercial protein-rich plant products. Plant Foods Hum Nutr 73:108–115CrossRefGoogle Scholar
  5. 5.
    Thambiraj SR, Phillips M, Koyyalamudi SR, Reddy N (2018) Yellow lupin (Lupinus luteus L.) polysaccharides: antioxidant, immunomodulatory and prebiotic activities and their structural characterization. Food Chem 267:319–328CrossRefGoogle Scholar
  6. 6.
    Foley RC, Gao L-L, Spriggs A, Soo LYC, Goggin DE, Smith PMC, Craig AA, Singh KB (2011) Identification and characterization of seed storage protein transcripts from Lupinus angustifolius. BMC Plant Biol 15(106):1–12Google Scholar
  7. 7.
    Lovati MR, Manzoni C, Castiglioni S, Parolari A, Magni C, Duranti M (2012) Lupin seed γ-conglutin lowers blood glucose in hyperglycaemic rats and increases glucose consumption of HepG2 cell. Br J Nutr 107(1):67–73CrossRefGoogle Scholar
  8. 8.
    D’agostina A, Antonioni C, Resta D, Arnoldi A, Bez J, Knauf U, Wäsche A (2006) Optimization of a pilot-scale process for producing lupin protein isolates with valuable technological properties and minimum thermal damage. J Agric Food Chem 54:92–98CrossRefGoogle Scholar
  9. 9.
    Piornos JA, Burgos-Díaz C, Ogura T, Morales E, Rubilar M, Maureira-Butler I, Salvo-Garrido H (2015) Functional and physicochemical properties of a protein isolate from AluProt-CGNA: a novel protein-rich lupin variety. Food Res Int 76:719–724CrossRefGoogle Scholar
  10. 10.
    Alamanou S, Bloukas JG, Paneras ED, Doxastakis G (1996) Influence of protein isolate from lupin seeds (Lupinus albus ssp. Graecus) on processing and quality characteristics of frankfurters. Meat Sci 42(1):79–93CrossRefGoogle Scholar
  11. 11.
    Burgos-Díaz C, Piornos JA, Wandersleben T, Ogura T, Hernández X, Rubilar M (2016) Emulsifying and foaming properties of different protein fractions obtained from a novel lupin variety AluProt-CGNA® (Lupinus luteus). J Food Sci 81:1699–1706CrossRefGoogle Scholar
  12. 12.
    Jezierny D, Mosenthin R, Sauer N, Roth S, Piepho H-P, Rademacher M, Eklund M (2011) Chemical composition and standardised ileal digestibilities of crude protein and amino acids in grain legumes for growing pigs. Livest Sci 138:229–243CrossRefGoogle Scholar
  13. 13.
    Oser BL (1959) An integrated essential amino acid index for predicting biological value of proteins. In: Albanese AA (ed) Protein and amino acid nutrition. Academic Press, New York, pp 295–311Google Scholar
  14. 14.
    Alsmeyer RH, Cunningham AE, Happich ML (1974) Equations to predict PER from amino acid analysis. Food Technol 28:24–38Google Scholar
  15. 15.
    Sathe SK, Deshpande SS, Salunkhe DK (1982) Functional properties of lupin seed (Lupinus mutabilis) proteins and protein concentrates. J Food Sci 47(2):491–497CrossRefGoogle Scholar
  16. 16.
    Berghout JAM, Boom RM, van der Goot AJ (2014) The potential of aqueous fractionation of lupin seeds for high-protein foods. Food Chem 159:64–70CrossRefGoogle Scholar
  17. 17.
    Hickisch A, Bindl K, Vogel RF, Toelstede S (2016) Thermal treatment of lupin-based milk alternatives – impact on lupin proteins and the network of respective lupin-based yogurt alternatives. Food Res Int 89:850–859CrossRefGoogle Scholar
  18. 18.
    Chew PG, Casey AJ, Johnson SK (2003) Protein quality and physio-functionality of Australian sweet lupin (Lupinus angustifolius cv. Gungurru) protein concentrates prepared by isoelectric precipitation or ultrafiltration. Food Chem 83:575–583CrossRefGoogle Scholar
  19. 19.
    Boye J, Wijesinha-Bettoni R, Burlingame B (2012) Protein quality evaluation twenty years after the introduction of the protein digestibility corrected amino acid score method. Br J Nutr 108:S183–S211CrossRefGoogle Scholar
  20. 20.
    Abdul-Hamid A, Bakar J, Hock-Bee G (2002) Nutritional quality of spray dried protein hydrolysate from black Tilapia (Oreochromis mossambicus). Food Chem 78(1):69–74CrossRefGoogle Scholar
  21. 21.
    WHO/FAO/UNU (2007) Protein and amino acid requirements in human nutrition: Report of a joint WHO/FAO/UNU. Expert consultation. WHO technical report series No. 935. GenevaGoogle Scholar
  22. 22.
    Sujak A, Kotlarz A, Strobel W (2006) Compositional and nutritional evaluation of several lupin seeds. Food Chem 98:711–719CrossRefGoogle Scholar
  23. 23.
    Shaheen N, Islam S, Munmun S, Mohiduzzaman M, Longvah T (2016) Amino acid profile and digestibility indispensable amino acid scores of proteins from the prioritized key foods in Bangladesh. Food Chem 213:83–89CrossRefGoogle Scholar
  24. 24.
    Morris CR, Hamilton-Reeves J, Martindale RG, Sarav M, Gautier JB (2017) Acquired amino acid deficiencies: a focus on arginine and glutamine. Nutr Clin Pract 32(1):30S–47SCrossRefGoogle Scholar
  25. 25.
    Tripathi P, Misra MK, Pandey S (2012) Role of L-arginine on dyslipidemic conditions of acute myocardial infarction patients. Ind J Clin Biochem 27(3):296–299CrossRefGoogle Scholar
  26. 26.
    Fontanari GG, Batistuti JP, da Cruz RJ, Nascimento PH, Gomes JA (2012) Cholesterol-lowering effect of whole lupin (Lupinus albus) seed and its protein isolate. Food Chem 132(3):1521–1526CrossRefGoogle Scholar
  27. 27.
    Udenigwe CC, Aluko RE (2012) Food protein-derived bioactive peptides: production, processing, and potential health benefits. J Food Sci 77:R11–R24CrossRefGoogle Scholar
  28. 28.
    Astorga-España MS, Rodríguez-Galdón B, Rodríguez-Rodríguez EM, Díaz-Romero C (2016) Amino acid content in seaweeds from the Magellan Straits (Chile). J Food Compos Anal 53:77–84CrossRefGoogle Scholar
  29. 29.
    Young VR, Pellett PL (1994) Plant proteins in relation to human protein and amino acid nutrition. Am J Clin Nutr 59:1203S–1212SCrossRefGoogle Scholar
  30. 30.
    Hoffman JR, Falvo MJ (2004) Protein—Which is best? J Sports Sci Med 3(3):118–130PubMedPubMedCentralGoogle Scholar
  31. 31.
    El-Adawy TA, Rahma EH, El-Bedawey AA, Gafar AF (2001) Nutritional potential and functional properties of sweet and bitter lupin seed protein isolates. Food Chem 74:455–462CrossRefGoogle Scholar
  32. 32.
    FAO (2013) Dietary protein quality evaluation in human nutrition. Report of an FAO expert consultation. Rome, ItalyGoogle Scholar
  33. 33.
    Mathai JK, Liu Y, Stein HH (2017) Values for digestible amino acid scores (DIASS) for some dairy and plant proteins may better describe protein quality than values calculated using the concept for protein digestible-corrected amino acid scores (PDCASS). Br J Nutr 117:490–499CrossRefGoogle Scholar
  34. 34.
    Lizarazo CI (2017). Diversifying boreal – nemoral cropping systems for sustainable protein product. PhD Thesis, University of HelsinkyGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • César Burgos-Díaz
    • 1
    Email author
  • Mauricio Opazo-Navarrete
    • 1
  • Traudy Wandersleben
    • 2
  • Monserrat Soto-Añual
    • 1
  • Tamara Barahona
    • 1
  • Mariela Bustamante
    • 3
  1. 1.Agriaquaculture Nutritional Genomic CenterCGNATemucoChile
  2. 2.Department of Chemical Sciences and Natural ResourcesUniversidad de La FronteraTemucoChile
  3. 3.Center of Food Biotechnology and Bioseparations, Scientific and Technological Bioresource Nucleus, BIOREN, and Department of Chemical EngineeringUniversidad de La FronteraTemucoChile

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