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Plant Foods for Human Nutrition

, Volume 74, Issue 4, pp 508–517 | Cite as

Chemical and Nutritional Evaluation of Protein-Rich Ingredients Obtained through a Technological Process from Yellow Lupin Seeds (Lupinus luteus)

  • César Burgos-DíazEmail author
  • Mauricio Opazo-Navarrete
  • Traudy Wandersleben
  • Monserrat Soto-Añual
  • Tamara Barahona
  • Mariela Bustamante
Original Paper

Abstract

In recent years, interest in plant-based proteins has been rising due to ethical and sustainability issues. In this context, the production of protein concentrates and isolates from new plant sources have increased enormously because of their nutritional and techno-functional properties. Therefore, this work describes a pilot process for obtaining protein-rich ingredients from a yellow lupin variety (Lupinus luteus) developed by the Agriaquaculture Nutritional Genomic Center (CGNA). A protein alkaline solubilisation followed by isoelectric precipitation was used as a method for obtaining a protein concentrate (LPC) and isolate (LPI) with 75 and 95% protein, respectively. The changes in the protein quality and chemical composition during the protein concentration process from lupin flour were evaluated. Thus, nutritional parameters such as the amino acids (AAs) profile, essential amino acid index (EAAI), chemical score (CS), the protein efficiency ratio (PER), and digestible indispensable amino acid score (DIAAS) were determined to evaluate the nutritional quality of LPC and LPI. The facile and scalable protein isolation method without a defatting process proposed in this study showed a great protein separation efficiency (PSE), while the amino acids profile was not affected during both the concentration as well as the isolation of proteins. In addition, SDS-PAGE showed that both LPC and LPI mainly contained the high-molecular-weight proteins α and β-conglutins. Both LPC and LPI had a balanced amino acids profile, and arginine was the most predominant amino acid. These results are useful for increasing the use of lupin based-protein ingredients as a potential functional ingredient in the food industry.

Keywords

Lupinus luteus Protein isolate Rich-protein Amino acids Nutritional properties 

Abbreviations

AA

Amino acid

AAs

Amino acids

CP

Crude protein

CS

Chemical score

DIAAS

Digestible indispensable amino acid score

EAA

Essential amino acid

EAAs

Essential amino acids

EAAI

Essential amino acid index

LF

Lupin flour

LPC

Lupin protein concentrate

LPI

Lupin protein isolate

NFE

Nitrogen-free extract

PER

Protein efficiency ratio

PSE

Protein separation efficiency

SDS-PAGE

Sodium dodecyl sulphate polyacrylamide gel electrophoresis

SID

Standardised ileal digestibility

Notes

Acknowledgements

This research was supported by CONICYT through project N°R16F1002 (Programa de Fortalecimiento de Centros Regionales) and CONICYT-FONDEF/ ID18I10093. We acknowledge the Regional Government from La Araucanía Region (Chile).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • César Burgos-Díaz
    • 1
    Email author
  • Mauricio Opazo-Navarrete
    • 1
  • Traudy Wandersleben
    • 2
  • Monserrat Soto-Añual
    • 1
  • Tamara Barahona
    • 1
  • Mariela Bustamante
    • 3
  1. 1.Agriaquaculture Nutritional Genomic CenterCGNATemucoChile
  2. 2.Department of Chemical Sciences and Natural ResourcesUniversidad de La FronteraTemucoChile
  3. 3.Center of Food Biotechnology and Bioseparations, Scientific and Technological Bioresource Nucleus, BIOREN, and Department of Chemical EngineeringUniversidad de La FronteraTemucoChile

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