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t-Resveratrol Protects against Acute High Glucose Damage in Endothelial Cells

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Abstract

Postprandial hyperglycemia in diabetic and nondiabetic subjects is associated with endothelial dysfunction. Evidence shows that high glucose generates oxidative stress and a pro-inflammatory state promoting the development of cardiovascular diseases. trans-Resveratrol (t-RV) has been shown to reduce cardiovascular risk. To determine whether t-RV acts as a protector against acute high glucose (AHG)-induced damage, two in vitro models, rat aortic rings (RAR) and human umbilical vein endothelial cells (HUVEC) were used. RAR pretreated with AHG (25 mM D-glucose) for 3 h dramatically decreased the endothelium-dependent relaxation (EDR) induced by acetylcholine in phenylephrine (PE)-precontracted vessels. However, coincubation with t-RV significantly mitigated the damage induced by AHG on EDR. Pretreatment with AHG did not affect the vasodilation induced by sodium nitroprusside. HUVEC treated with t-RV decreased cytotoxicity and reduced radical oxygen species production induced by AHG. Taken together, these results suggest that t-RV can mitigate the AHG-induced EDR damage through a mechanism involving ROS scavenging and probably an increase in the bioavailability of NO.

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Abbreviations

ACh:

Acetylcholine

AHG:

Acute high glucose

EC50 :

The concentration of the compound that gives half-maximal response

EDR:

Endothelium-dependent relaxation

Emax:

Emax maximal effect (relaxation)

HUVEC:

Human umbilical vein endothelial cells

KHB:

Krebs-Henseleit buffer

MTS:

3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium

NO:

Nitric oxide

PE:

Phenylephrine

RAR:

Rat aortic rings

ROS:

Reactive oxygen species

SNP:

Sodium nitroprusside

t-RV:

trans-Resveratrol

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Acknowledgements

This work was supported by Grants DIUV No. 57/2011 from Universidad de Valparaíso, Chile; Grant CREAS No. R12C1001 from CONICYT-REGIONAL, GORE Región de Valparaíso, Chile; Grant No. 037-274 from Pontificia Universidad Católica de Valparaíso, Chile and Grant DICYT-USACH No. 021643MS from JLM.

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Authors and Affiliations

  1. Laboratory of Biological Chemistry, Institute of Chemistry, Faculty of Sciences, Pontificia Universidad Católica de Valparaíso, 2373223, Valparaíso, Chile

    Leda Guzmán & Cristóbal Balada

  2. Laboratory of Pharmacology, Faculty of Pharmacy, Universidad de Valparaíso, 2360102, Valparaíso, Chile

    Guillermo Flores, Rocío Álvarez, Marcela Knox & Raúl Vinet

  3. Centro de Investigación Farmacopea Chilena (CIFAR), 2360134, Valparaíso, Chile

    Rocío Álvarez & Raúl Vinet

  4. Regional Center for the Study in Foods and Health (CREAS), 2362696, Valparaíso, Chile

    Raúl Vinet

  5. Vice Presidency of Research, Development and Innovation, Universidad de Santiago de Chile, Estación Central, 9160000, Santiago, Chile

    José L. Martínez

Authors
  1. Leda Guzmán
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  2. Cristóbal Balada
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  3. Guillermo Flores
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  4. Rocío Álvarez
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  5. Marcela Knox
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  7. José L. Martínez
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Correspondence to José L. Martínez.

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Guzmán, L., Balada, C., Flores, G. et al. t-Resveratrol Protects against Acute High Glucose Damage in Endothelial Cells. Plant Foods Hum Nutr 73, 235–240 (2018). https://doi.org/10.1007/s11130-018-0683-0

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