Comparative Analysis of Native Crocus Taxa as a Great Source of Flavonoids with High Antioxidant Activity
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Native Crocus taxa are abundant in regions with moderate and dry climate; however, their potential in the food industry has thus far been neglected. The objective of this study was to compare the quantitative and qualitative flavonoid content and antioxidant and cytotoxic activity in organs of several populations of Croatian native taxa and assess their potential for application in the food industry. The dominant flavonoids in the native Crocus taxa were kaempferol and quercetin. Tepals contained similar or higher concentrations of flavonoid-glycosides than other organs. Tepals from Cvv1 and Cvv2 populations contained more flavonoid-glycosides than the commonly used spice saffron (C. sativus stigmas). The FRAP antioxidant activity of Cvv1 and Cvv5 tepals was similar to that of standard Trolox. DPPH inhibition of Cvv1 and Cvv3 tepals was within the range of that recorded for saffron. Cvv1 tepals significantly reduced reactive oxygen species in the broadest concentration range (50–1000 μg/ml), and showed considerable antioxidant activity in the ABTS assay, equal to 82% of standard Trolox antioxidant activity. A significantly higher concentration of kaempferol-rutinoside was recorded in this than in other taxa. The flavonoid showed a very strong or strong correlation with antioxidant assays results, and a negative correlation with cellular reactive oxygen species concentration. We therefore presumed that kaempferol-rutinoside is one of the main antioxidant phenolics in Crocus tepals. None of the tested extracts showed cytotoxicity toward Caco-2 cells. The results revealed that Cvv tepals have potential as a food supplement and are a promising material for further food safety tests.
KeywordsCrocus malyi Crocus vernus ssp. albiflorus Crocus vernus ssp. vernus Food potential Kaempferol-rutinoside RP-HPLC
Crocus malyi (location North Velebit)
Crocus vernus ssp. albiflorus (location Jelenje, Delnice)
Crocus vernus ssp. vernus (location Botanical Garden, Zagreb)
Crocus vernus ssp. vernus (location Čulinec, Zagreb)
Crocus vernus ssp. vernus (location Grad mladih, Zagreb)
Crocus vernus ssp. vernus (location Čazma)
Crocus vernus ssp. vernus (location North Velebit)
ferric reducing antioxidant power
reactive oxygen species
- tot. aglyc.
- tot. glyc.
The authors thank Ksenija Durgo and Dario Hruševar for help with cytotoxic test and collecting part of the plant material in this study. The study was financially supported by the University of Zagreb (Grants No. 20281217 and 000070002401), Croatia.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 1.Moratalla-López N, Lorenzo C, Alonso GL, Sánchez AM (2016) Kaempferol glycosides in Crocus: sources, biosynthesis, and uses. In: Garde-Cerdán T, Gonzalo-Diago A (eds) Biosynthesis, food sources and therapeutic uses, 3rd edn. Nova Science Publishers, New York, pp 151–196Google Scholar
- 2.Petersen G, Seberg O, Thorsøe S, Tina Jørgensen T, Mathew B (2008) A phylogeny of the genus Crocus (Iridaceae) based on sequence data from five plastid regions. Taxon 57:487–499Google Scholar
- 8.Goli SAH, Mokhtari F, Rahimmalek M (2012) Phenolic compounds and antioxidant activity from saffron (Crocus sativus L.) petal. J Agric Sci 4:175–181Google Scholar
- 10.Ordoudi SA, Tsimidou MZ (2004) Saffron quality: Effect of agricultural practices, processing and storage. In: Dris R, Mohan Jain S (eds) Production practices and quality assessment of food crops. Volume 1 – Preharvest practice. Kluwer Academic Publishers, Dordrecht, pp 209–260Google Scholar
- 18.Ahmadian-Kouchaksaraie Z, Niazmand R, Najafi MN (2016) Optimization of the subcritical water extraction of phenolic antioxidants from Crocus sativus petals of saffron industry residues: Box-Behnken design and principal component analysis. Innov Food Sci Emerg Technol 36:234–244CrossRefGoogle Scholar
- 28.Evans JD (1996) Straightforward statistics for the behavioral sciences. Brooks/Cole Publishing, Pacific GroveGoogle Scholar