Colorimetric Analysis of Hibiscus Beverages and their Potential Antioxidant Properties
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In food industry, roselle beverages and their subproducts could be functional ingredients since they are an excellent source of bioactive compounds with improved performance due to their important anthocyanins content. The aim of this study was to analyze anthocyanin content and antioxidant properties of aqueous infusions elaborated with color contrasting Hibiscus materials and design a mathematical model in order to predict color-composition relationship. Color measurements of beverages from roselle (Negra, Sudan and Rosa) were made by transmission spectrophotometry, anthocyanins quantification was determined by HPLC, and antioxidant potential was evaluated by in vitro methods (ABTS and FRAP assays). Beverages prepared with particle size minor of 250 μm presented until 4- and 2- times more anthocyanins content and antioxidant capacity respectively, in comparison to beverages prepared with powders with particle size major of 750 μm. Positive correlations among pigments composition and color parameters were found (p < 0.05), showing that anthocyanins content, antioxidant capacity, C*ab and hab values increased in relation with the smallest particle size of flours. Also, mathematical models were stablished to predict anthocyanin content (r ≥ 0.97) and antioxidant capacity (r ≥ 0.89) from color data; we propose equations for quick estimation of the antioxidant capacity in the Hibiscus beverages with high anthocyanin content. The obtained models could be an important tool to be used in food industry for pigment characterization or functional compounds with potential health benefits.
KeywordsHibiscus beverages Anthocyanins Antioxidant capacity Tristimulus colorimetry
The authors thank to Colegio Superior de Agricultura del Estado de Guerrero (CSAEGRO) and Quintín Obispo González for biological samples. Also, thank the support from CONACYT, Mexico, SIP-IPN, COFAA-IPN, and EDI-IPN. One of the authors (GACM) also acknowledges the scholarship from CONACYT, Mexico.
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Conflict of Interest
We certify that there is no actual or potential conflict of interest in relation of this article.
- 1.Gomes Maganha E, da Costa Halmenschlager R, Moreira Rosa R, Pegas Henriques JA, Lia de Paula Ramos AL, Saffi J (2010) Pharmacological evidences for the extracts and secondary metabolites from plants of the genus Hibiscus. Food Chem 118:1–10. https://doi.org/10.1016/j.foodchem.2009.04.005 CrossRefGoogle Scholar
- 2.Camelo-Méndez GA, Jara-Palacios MJ, Escudero-Gilete ML, Gordillo B, Hernanz D, Paredes-López O, Vanegas-Espinoza PE, Del Villar-Martínez AA, Heredia FJ (2016) Comparative study of phenolic profile, antioxidant capacity, and color-composition relation of Roselle cultivars with contrasting pigmentation. Plant Foods Hum Nutr 71:109–114. https://doi.org/10.1007/s11130-015-0522-5 CrossRefPubMedGoogle Scholar
- 4.Ramírez-Rodrigues MM, Plaza ML, Azeredo A, Balaban MO, Marshall MR (2012) Phytochemical, sensory attributes and aroma stability of dense phase carbon dioxide processed Hibiscus sabdariffa beverage during storage. Food Chem 134:1425–1431. https://doi.org/10.1016/j.foodchem.2012.03.042 CrossRefPubMedGoogle Scholar
- 5.Camelo-Méndez GA, Ragazzo-Sánchez JA, Jiménez-Aparicio AR, Vanegas-Espinoza PE, Paredes-López O, Del Villar-Martínez AA (2013) Comparative study of anthocyanin and volatile compounds content of four varieties of Mexican roselle (Hibiscus sabdariffa L.) by multivariable analysis. Plant Foods Hum Nutr 68:229–234. https://doi.org/10.1007/s11130-013-0360-2 CrossRefPubMedGoogle Scholar
- 6.Maga JA, Kim CH (1990) Stability of natural colorants (annatto, beet, paprika, turmeric) during extrusion cooking. Lebensm Wiss Technol 23:427–432Google Scholar
- 8.Hernanz D, Recamales ÁF, Meléndez-Martínez AJ, González-Miret ML, Heredia FJ (2008) Multivariate statistical analysis of the color - anthocyanin relationships in different soilless-grown strawberry genotypes. J Agric Food Chem 56:2735–2741. https://doi.org/10.1021/jf073389j CrossRefPubMedGoogle Scholar
- 10.Jiménez AM, Sierra CA, Rodríguez-Pulido FJ, González-Miret ML, Heredia FJ, Osorio C (2011) Physicochemical characterisation of gulupa (Passiflora edulis Sims. Fo edulis) fruit from Colombia during the ripening. Food Res Int 44:1912–1918. https://doi.org/10.1016/j.foodres.2010.11.007 CrossRefGoogle Scholar
- 13.Heredia FJ, Álvarez C, GonzÁlez-Miret ML, Ramírez A (2004) CromaLab, anÁlisis de color. Registro General de la Propiedad IntelectualGoogle Scholar
- 14.CIE (2004) Technical report colorimetry. Commission Internationale de l’Eclairage Central Bureau, Vienna, AustriaGoogle Scholar
- 15.Wyszecki G, Stiles WS (1982) Color science. Concepts and methods. Quantitative data and formulae, 2nd edn. John Wiley and Sons, New YorkGoogle Scholar