Differences in possible TCA cycle replenishing pathways in purple non-sulfur bacteria possessing glyoxylate pathway
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Pathways replenishing tricarboxylic acid cycle were divided into four major groups based on metabolite serving as source for oxaloacetic acid or other tricarboxylic acid cycle component synthesis. Using this metabolic map, the analysis of genetic potential for functioning of tricarboxylic acid cycle replenishment pathways was carried out for seven strains of purple non-sulfur bacterium Rhodopseudomonas palustris. The results varied from strain to strain. Published microarray data for phototrophic acetate cultures of Rps. palustris CGA009 were analyzed to validate activity of the putative pathways. All the results were compared with the results for another purple non-sulfur bacterium, Rhodobacter capsulatus SB1003 and species-specific differences were clarified.
KeywordsRhodopseudomonas palustris Purple bacteria Acetate assimilation Tricarboxylic acid cycle replenishing pathways Anaplerotic pathway Glyoxylate cycle
- TCA cycle
Tricarboxylic acid cycle
The authors are grateful to Dr. Azat V. Abdullatypov for consulting, productive discussion, and careful revision of the manuscript. This work was conducted in the frame of project “Photosynthetic organisms as light energy transformers and valuable products producers” number АААА-А17-117030110141-2.
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Conflict of interest
The authors declare that they have no conflicts of interest.
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