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Photosynthesis Research

, Volume 139, Issue 1–3, pp 359–365 | Cite as

Significance of PGR5-dependent cyclic electron flow for optimizing the rate of ATP synthesis and consumption in Arabidopsis chloroplasts

  • Ryoichi Sato
  • Rinya Kawashima
  • Mai Duy Luu Trinh
  • Masahiro Nakano
  • Takeharu Nagai
  • Shinji MasudaEmail author
Original Article

Abstract

The proton motive force (PMF) across the chloroplast thylakoid membrane that is generated by electron transport during photosynthesis is the driving force for ATP synthesis in plants. The PMF mainly arises from the oxidation of water in photosystem II and from electron transfer within the cytochrome b6f complex. There are two electron transfer pathways related to PMF formation: linear electron flow and cyclic electron flow. Proton gradient regulation 5 (PGR5) is a major component of the cyclic electron flow pathway, and the Arabidopsis pgr5 mutant shows a substantial reduction in the PMF. How the PGR5-dependent cyclic electron flow contributes to ATP synthesis has not, however, been fully delineated. In this study, we monitored in vivo ATP levels in Arabidopsis chloroplasts in real time using a genetically encoded bioluminescence-based ATP indicator, Nano-lantern(ATP1). The increase in ATP in the chloroplast stroma of pgr5 leaves upon illumination with actinic light was significantly slower than in wild type, and the decrease in ATP levels when this illumination stopped was significantly faster in pgr5 leaves than in wild type. These results indicated that PGR5-dependent cyclic electron flow around photosystem I helps to sustain the rate of ATP synthesis, which is important for growth under fluctuating light conditions.

Keywords

Photosynthesis PGR5 ATP indicator Nano-lantern Cyclic electron flow Chloroplast 

Supplementary material

11120_2018_533_MOESM1_ESM.pdf (1.2 mb)
Supplementary Figures (PDF 1250 KB)
11120_2018_533_MOESM2_ESM.mov (2.1 mb)
Supplementary movie (MOV 2157 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.School of Life Science and TechnologyTokyo Institute of TechnologyYokohamaJapan
  2. 2.The Institute of Scientific and Industrial ResearchOsaka UniversityOsakaJapan
  3. 3.Center for Biological Resources & InformaticsTokyo Institute of TechnologyYokohamaJapan
  4. 4.Division of Environmental PhotobiologyNational Institute for Basic BiologyOkazakiJapan

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