OsSGT1 Is a Glucosyltransferase Gene Involved in the Glucose Conjugation of Phenolics in Rice
- 532 Downloads
Phenolics are a class of plant secondary metabolites that play important roles in plant growth and environmental adaptation. Glucosylation of phenolics is one of the molecular mechanisms controlling phenolics homeostasis. However, the relevant glucosyltransferases are largely unknown. In this study, a putative family 1 glucosyltransferase gene OsSGT1 was cloned from rice due to its close homology with the previously reported phenolics-related glucosyltransferases UGT84A1-A4, and the phylogenetic relationship of OsSGT1 with homologs from other species was investigated. Recombinant OsSGT1 protein showed strong activity towards phenolics to form their glucose conjugates. This is the first identified natural phenolics-related glucosyltransferase in rice. In addition, the expression patterns of OsSGT1 in different tissues of rice indicated that OsSGT1 was predominantly expressed in the old leaves and dough grains, suggesting that OsSGT1 might be involved in the maturation process of rice by regulating phenolic metabolism, and thus deepened our understanding on the roles of phenolics in rice growth and environmental adaptation.
KeywordsOriza stiva Glucosyltransferase Glycosylation Phenolics Enzyme activity Expression pattern
BKH and QL conceived and designed the research. QL, YYZ, and LC conducted the experiments. TTC and YJL contributed analytical tools and analyzed data. QL, TTC, and BKH wrote the manuscript. All authors read and approved the manuscript.
This study was financially supported by key R & D project of Shandong Province of China (no. 2018GNC110019).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- Blomstedt C-K, O'Donnell N-H, Bjarnholt N, Neale A-D, Hamill J-D, Møller B-L, Gleadow R-M (2016) Metabolic consequences of knocking out UGT85B1, the gene encoding the glucosyltransferase required for synthesis of dhurrin in Sorghum bicolor (L. Moench). Plant Cell Physiol 57:373–386CrossRefPubMedGoogle Scholar
- Ross J, Li Y, Lim E, Bowles D-J (2001) Higher plant glycosyltransferases. Genome Biol 2(3004):1–6Google Scholar
- Yonekura-Sakakibara K, Fukushima A, Nakabayashi R, Hanada K, Matsuda F, Sugawara S, Inoue E, Kuromori T, Ito T, Shinozaki K, Wangwattana B, Yamazaki M, Saito K (2012) Two glycosyltransferases involved in anthocyanin modification delineated by transcriptome independent component analysis in Arabidopsis thaliana. Plant J 69:154–167CrossRefPubMedGoogle Scholar