Plant Molecular Biology Reporter

, Volume 37, Issue 1–2, pp 41–49 | Cite as

OsSGT1 Is a Glucosyltransferase Gene Involved in the Glucose Conjugation of Phenolics in Rice

  • Qian Liu
  • Yu-ying Zhang
  • Lu Chen
  • Ting-ting Chen
  • Yan-jie Li
  • Bing-kai HouEmail author
Original Paper


Phenolics are a class of plant secondary metabolites that play important roles in plant growth and environmental adaptation. Glucosylation of phenolics is one of the molecular mechanisms controlling phenolics homeostasis. However, the relevant glucosyltransferases are largely unknown. In this study, a putative family 1 glucosyltransferase gene OsSGT1 was cloned from rice due to its close homology with the previously reported phenolics-related glucosyltransferases UGT84A1-A4, and the phylogenetic relationship of OsSGT1 with homologs from other species was investigated. Recombinant OsSGT1 protein showed strong activity towards phenolics to form their glucose conjugates. This is the first identified natural phenolics-related glucosyltransferase in rice. In addition, the expression patterns of OsSGT1 in different tissues of rice indicated that OsSGT1 was predominantly expressed in the old leaves and dough grains, suggesting that OsSGT1 might be involved in the maturation process of rice by regulating phenolic metabolism, and thus deepened our understanding on the roles of phenolics in rice growth and environmental adaptation.


Oriza stiva Glucosyltransferase Glycosylation Phenolics Enzyme activity Expression pattern 



BKH and QL conceived and designed the research. QL, YYZ, and LC conducted the experiments. TTC and YJL contributed analytical tools and analyzed data. QL, TTC, and BKH wrote the manuscript. All authors read and approved the manuscript.

Funding Information

This study was financially supported by key R & D project of Shandong Province of China (no. 2018GNC110019).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

11105_2019_1134_MOESM1_ESM.docx (157 kb)
Fig S1 HPLC analysis of reaction mixtures of different phenolics with heat-inactivated OsSGT1 as negative control. (a-e) denote sinapic acid, ferulic acid, caffeic acid, cinnamic acid and coumaric acid, respectively; 1 denotes the reactions with inactivated GST-OsSGT1. 2 denotes the authentic standards of phenolics. (DOCX 157 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Qian Liu
    • 1
  • Yu-ying Zhang
    • 1
  • Lu Chen
    • 1
  • Ting-ting Chen
    • 1
  • Yan-jie Li
    • 1
  • Bing-kai Hou
    • 1
    Email author
  1. 1.The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education; School of Life ScienceShandong UniversityQingdaoChina

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